Depletion of adult hippocampal neurogenesis increases anxiety-related behaviors in the elevated plus maze (EPM) and in the light/dark emergence test. (a) Illustrations of the 3D spatial distribution of the exploratory path in bigenic-Dox mice, in which neurogenesis has been inhibited, and in control bigenic-vehicle mice. During the EPM test, bigenic-Dox mice (in comparison to control bigenic-vehicle mice): (b) spent less time in the open arms (t16=5.554, P=0.00004); (c) covered less distance in the open arms (t16=2.219, P=0.041); (d) visited the extremities of the open arms fewer times (t16=2.162, P=0.046). In the light/dark emergence test, bigenic-Dox mice (in comparison to control bigenic-vehicle mice): (e) showed a higher initial latency to emerge from the protective cylinder (t16=−2.93, P=0.009); (f) spent more time in the protective cylinder (t16=−2.16, P<0.05); (g) had a higher mean time per entry into the cylinder (t16=−3.67, P<0.01). *P<0.05; **P<0.01; ***P<0.001 in comparison to the bigenic vehicle controls. White bar=bigenic-vehicle mice. Black bar=bigenic-Dox mice.