Decrease in neurogenesis in the adult dentate gyrus (DG) of bigenic animals treated with Dox. (a) Number of HH3-IR cells (t16=1.8, P=0.04). Insert: illustration of a HH3-IR cells. Bar scale=10 μm. (b) Number of Dcx-IR (t15=2.86, P=0.01). Illustration of Dcx-IR immature neurons. Bar scale=50 μm. (c) Illustration of Dcx-IR immature neurons at a higher magnification. Bar scale=10 μm. Sholl analysis of Big-Veh and Big-Dox neurons (group effect: F1,6=0.07, P=0.79; group x level interaction: F25,150=0.45, P=0.99). *P<0.05; **P<0.01 in comparison to the control group. White bar=bigenic-vehicle mice. Black bar=bigenic-Dox mice.