Mucosal surfaces are under constant bombardment from potentially antigenic particles and so must maintain a balance between homeostasis and inappropriate immune activation and consequent pathology. Epithelial cells have a vital role orchestrating pulmonary homeostasis and defense against pathogens. TGF-β regulates an array of immune responses—both inflammatory and regulatory—however, its function is highly location- and context-dependent. We demonstrate that epithelial-derived TGF-β acts as a pro-viral factor suppressing early immune responses during influenza A infection. Mice specifically lacking bronchial epithelial TGF-β1 (epTGFβKO) displayed marked protection from influenza-induced weight loss, airway inflammation, and pathology. However, protection from influenza-induced pathology was not associated with a heightened lymphocytic immune response. In contrast, the kinetics of interferon beta (IFNβ) release into the airways was significantly enhanced in epTGFβKO mice compared with control mice, with elevated IFNβ on day 1 in epTGFβKO compared with control mice. This induced a heighted antiviral state resulting in impaired viral replication in epTGFβKO mice. Thus, epithelial-derived TGF-β acts to suppress early IFNβ responses leading to increased viral burden and pathology. This study demonstrates the importance of the local epithelial microenvironmental niche in shaping initial immune responses to viral infection and controlling host disease.
Pulmonary epithelial cells are continually exposed to an inhaled environment comprising billions of innocuous particles as well as potential pathogens. Interactions between epithelial cells of the conducting airways and environmental stimuli are important in the development of pulmonary pathology, and perturbations in the level of expression of the pleiotropic cytokine tumor growth factor (TGF)-β and related signaling molecules in these cells appear to be pivotal. Altered expression of the TGF-β signaling molecule smad 2 at the mucosal surface has previously been shown to drive airway remodeling in response to allergen via modulation of innate epithelial-derived mediators.1, 2 Epithelial cell-derived TGF-β has also been shown to be a critical cofactor for enhanced innate lymphoid cell function and generation of allergen-mediated allergic airway diseases.3 However, the precise role of TGF-β of epithelial origin in shaping the immune response to viruses within the respiratory tract is less clear. Seasonal infections with the respiratory virus influenza A result in significant mortality and morbidity in vulnerable groups as well as substantial healthcare and economic costs in the healthy population.4 Epithelial cells sense infection by pathogens via recognition of pathogen-associated molecular patterns and damage-associated molecular pattern molecules. Activation of these pathways induces rapid release of epithelial-derived type I (interferon alpha and beta (IFNα and IFNβ, respectively)) and type III IFNs (IFNλ), which are vital for antiviral immunity.
IFN signaling in adjacent epithelial cells activates a cascade of IFN-stimulated genes (ISGs) resulting in neighboring epithelial cells adopting an “antiviral state”, reducing initial viral replication and subsequent viral spread.5 Rapid induction of the antiviral state is crucial as it reduces viral burden allowing time for development of adaptive immune responses.
Expression of TGF-β is increased in the pulmonary epithelium after infection with influenza;6, 7 however, our understanding of the function of this pool of bioactive TGF-β is limited. However, work utilizing rhinovirus infection of human epithelial in vitro culture systems indicates an immune-suppressive function for TGF-β.8
In contrast, we show that mice specifically lacking epithelial TGF-β (epTGFβKO) exhibited marked protection from influenza-induced weight loss and airway inflammation concomitant with significantly reduced pathology and viral burden. We describe the very early events at the initial site of infection (the bronchiolar epithelium) that shape and direct the subsequent immune response and place the relationship between TGF-β and interferon as a key determinant of host outcome.
Epithelial TGF-β promotes influenza-induced pathology
Mice specifically lacking expression of TGF-β1 in club cells that comprise 80% of bronchiolar epithelial cells were generated by crossing TGF-β1fl/fl mice with CCSP Tet-on CRE mice (Figure 1a).3 After the mice reached adulthood, they were administered a single dose of doxycycline (DOX) inducing Cre recombinase expression in epithelial club cells and subsequent excision of TGF-β, generating epTGFβKO. A detailed description of the generation of these mice and the methods used to establish ablation of epithelial TGF-β was included in Denney et al.3 Littermates that lack one of the genes essential for excision of TGF-β were utilized as control mice. As previously reported, at steady-state epTGFβKO mice displayed no inflammatory pathology3 in stark contrast to mice lacking global or T-cell-specific TGF-β or TGF-β signaling.9
To investigate the role of local epithelial TGF-β secretion during the pulmonary response to virus, epTGFβKO mice were infected with (1.2 × 104 TCID50 per 50 μl) influenza A (subtype X31 H3N2). Although control mice showed characteristic weight loss associated with influenza infection, we found that epTGFβKO mice were strongly protected from weight loss (Figure 1b), and showed only a minimal weight change from mock-infected mice on day 3 post infection. epTGFβKO mice were also protected from pathological changes within lung tissue induced by influenza infection (Figure 1c,d). Indeed, this protection was apparent very early during the disease course. The bronchiolar epithelium of control mice on day 1 post infection displayed more areas of damaged epithelial cells with sections of ruffling and disruption to normal columnar organization than mice lacking epithelial TGF-β. Associated airway inflammation was also markedly reduced in epTGFβKO mice with fewer infiltrating cells on day 3 and 6 post infection compared with infected control mice (Figure 1e).
Influenza infection is known to increase levels of bioactive TGF-β and, as mice lacking epithelial TGF-β were protected from early pathological changes, we investigated the primary cellular sources of TGF-β in the lung. In control mice, increased levels of bioactive TGF-β were detected in the airway lumen from day 1 post infection (Figure 1f and Supplementary Figure 1a online). In contrast, this early increase in bioavailable TGF-β was not seen in mice lacking epithelial TGF-β, suggesting that during the initial immune response to influenza TGF-β is derived from bronchiolar epithelial cells rather than from immune or other stromal cells.
Next, in order to further investigate the potential sources of TGF-β after infection, we determined Tgfb1 mRNA transcript levels on day 1 post infection in both epithelial cells and cells from the airway lumen (Figure 1g,h). Epithelial cells were obtained from tracheal brushings and, as expected, showed high relative expression of CCSP (Scgb1a1) transcript, strongly suggesting a club cell-enriched fraction (Supplementary Figure 1b). Airway lumen cells comprising resident and recruited leukocytes were obtained by via bronchoalveolar lavage (BAL). At 12 h post infection airway leukocytes comprise ∼95% airway macrophages (Supplementary Figure 1c). By 24 h post infection these cells included roughly equal proportions of (CD11c+SiglecF+) airway macrophages and neutrophils with a minority (<3%) lymphocytes and other cells as determined using flow cytometry (Supplementary Figure 1c).
At 12 h post infection Tgfb1 mRNA levels were not altered from homeostasis in either the epithelial cell or the airway leukocyte fraction (Supplementary Figure 1d,e). However, the epithelial cell fraction exhibited a significant increase in Tgfb1 mRNA expression from 24 h after infection compared with mock-infected mice, although this surge in transcript abundance was not observed in epTGFβKO mice (Figure 1g). In contrast, airway leukocyte Tgfb1 mRNA transcript levels were not modulated by either infection or ablation of epithelial TGF-β at 24 h post infection (Figure 1h).
We note that in control mice relative basal Tgfb1 transcript expression was much higher in the airway leukocytes (which comprise mostly airway macrophages) than epithelial cells (Supplementary Figure 1f). However, only epithelial cells displayed increased Tgfb1 mRNA expression in response to infection, which was attenuated in epTGFβKO mice suggesting de novo Tgfb1 transcription in bronchial club cells upon infection.
Muted cellular immune response in influenza-infected mice lacking epithelial TGF-β
Activation of resident airway macrophages as well as the early and efficient recruitment of other immune cells (neutrophils, infiltrating monocytes/macrophages, NK cells, T cell, and ILC1s) to the lung is vital for the generation of an effective antiviral inflammatory response and ultimately viral clearance. These cell types express TGF-βRII, and TGF-β has been shown to variously activate, recruit, maintain, and regulate these cell populations.9
There was a significant reduction in peak neutrophilia (day 3 post infection) in epTGFβKO mice (Figure 2a). The number of CD11c+ Siglec F+ airway macrophages increased in response to influenza infection within 24 h both in control and epTGFβKO mice (Figure 2b). However, while the magnitude of this response was further elevated at days 3 and 6 post infection in control mice, there was no additional increase in macrophage numbers in epTGFβKO mice. There was also no differential expression of activation markers (CD11b/MHCII) on airway macrophages between control and epTGFβKO mice at either homeostasis or after infection (Supplementary Figure 3A,B). Infiltrating monocytes and macrophages (SiglecFnegCD11cneg) are potent producers of IFNs and TNF-α and induce inflammation that promotes viral clearance; however, they can also cause severe immune pathology.10 Again, we observed a reduction in peak numbers of infiltrating monocyte and macrophages 6 days post-influenza challenge in epTGFβKO mice (Figure 2c).
Numbers of IFNγ+ NK cells and IFNγ+ ILC1s increased in response to influenza infection but were not affected by epithelial TGF-β (Supplementary Figure 2c,d. As expected, substantial numbers of IFNγ+ T cells were only induced on day 6 after infection with a reduction in both IFNγ+ CD8 and CD4 T cells seen in epTGFβKO mice, consistent with the diminished immune response to influenza infection observed in these mice (Supplementary Figure 2e,f). Interleukin (IL)-17+ CD4 T cells follow similar kinetics as seen with IFN-γ+ CD4 and CD8 T cells with a trend to reduced numbers on day 6 post infection (Supplementary Figure 2g). Similarly, on day 6 post infection, epTGFβKO mice showed a trend toward reduced numbers of Foxp3+ Tregs in the airways but not in the lung tissue (Supplementary Figure 3h,i).
Next, we examined the levels of key immune mediators (CCL2/MCP-1, CXCL1/KC, and IL-6) that could explain the differential cell numbers of inflammatory cells in the airways of infected epTGFβKO and control mice. CCL2/MCP-1 attracts monocytes, dendritic cells, and memory T cells; CXCL1/KC has neutrophil chemoattractant activity; and IL-6 is essential for neutrophil recruitment and function.11 In control mice these mediators all followed a similar pattern: peaking on day 3 and remaining elevated on day 6 (Figure 2d–f). There was a comparable induction of these mediators on day 1 in both epTGFβKO and control mice. However, on days 3 and 6 there was no further increase in the levels of CCL2 and KC in epTGFβKO mice and concentrations were significantly reduced on day 3 compared with control mice. Secretion of IL-6 was also significantly reduced in epTGFβKO mice on day 3, although by day 6 levels were comparable to control mice. The peak in IL-6 levels in the epTGFβKO mice on day 6 post infection is likely derived from a combination of recruited immune cell populations as well as structural cells (including epithelial cells).11, 12 IL-1β levels followed a similar pattern to IL-6 (Supplementary Figure 3j). In contrast to pro-inflammatory cytokines, IL-10 was not detected in the airways and in the lung tissue IL-10 levels were similar in both epTGFβKO and control mice (Supplementary Figure 3k).
Together, these data suggest a generally muted inflammatory response to influenza infection in epTGFβKO mice rather than reduced recruitment or activation of specific leukocyte populations in the absence of epithelial cell-derived TGF-β. It is therefore likely that these immune changes are a consequence of earlier events rather than the initial drivers of a protective mechanism.
Ablating epithelial TGF-β expression does not protect mice from respiratory syncytial virus-driven pathology
Next, we questioned whether this protection from pathology associated with the loss of epithelial TGF-β was restricted to influenza A infection, which is known to cleave inactive TGF-β and display distinct tropism for bronchial epithelium or was indicative of a more general association with viral immunity. Therefore, we infected mice with respiratory syncytial virus (RSV), which preferably infects alveolar epithelial cells and lacks the neuraminidase protein that mediates TGF-β activation by influenza virus.13 In stark contrast to influenza infection, epTGFβKO and control mice showed indistinguishable weight loss and airway inflammation following RSV infection (Figure 3a,b). In the airways, frequency of RSV tetramer+ CD8+T cells, NK cells, neutrophils, airway macrophages, and inflammatory monocyte/macrophages were equivalent in epTGFβKO mice and control (Figure 3c–g). Unlike influenza, RSV infection did not lead to increased levels of bioactive TGF-β in the airways on day 1 post infection and at all time points levels were similar in epTGFβKO and control mice (Figure 3h). In keeping with these data, Tgfb1 mRNA levels were not altered after RSV infection in either epithelial cells or airway leukocytes (Figure 3i,j). These data indicate the key importance to host outcome of both the site of viral infection as well as the capacity of the pathogen to alter the immediate immune environment by promoting the generation of bioactive TGF-β.
Ablation of epithelial TGF-β reduced live viral titer
As epTGFβKO mice exhibited a strong, early protection from the pathogenic effects of influenza infection, we examined whether the virus was able to infect bronchial epithelial cells in these mice. Influenza A initially infects club cells in the epithelium before subsequent infection of both structural and immune cells (including macrophages and DCs).14 We surveyed the location of viral particles within the lung at early time points after infection to determine primary infection sites. In keeping with published studies, infected cells were initially restricted to the bronchial epithelium and viral protein staining was observed in both control mice and those lacking epithelial TGF-β (Figure 4a), consistent with both control and TGF-β-deficient epithelium being permissive to the initial entry of influenza A virus (IAV) particles. Quantification of nucleoprotein (NP)-stained airways also showed reduced NP staining on day 3 post infection in epTGFβKO mice compared with control mice (Figure 4b). In order to further examine viral tropism, we determined the levels of viral RNA in epithelial cells and airway leukocytes finding that at 12 h and 1 day post infection X31 RNA levels are highest in epithelial cells (Supplementary Figure 4a). Although staining for NP protein and qPCR for viral RNA allows identification of infected cells and viral tropism, neither technique can quantitatively determine the live viral burden.
Therefore, we determined viral titer in whole-lung homogenate by plaque-forming unit assay.15 As expected, virus was detectable on day 1 post infection and plaque-forming unit peaked on day 3 in control mice (Figure 4c). In contrast, there was a significant reduction in plaque-forming unit in epTGFβKO mice on both day 1 and day 3 compared with control mice (Figure 4c). Therefore, mice lacking epithelial TGF-β had a substantially diminished early viral burden as well as a reduced peak viral load. These differences in viral titer on day 1 post infection suggest an altered early antiviral response in mice lacking epithelial TGF-β and likely accounts for the reduced immunopathology seen from day 3 onward.
Differential interferon responses mediate immune protection in mice lacking epithelial TGF-β
Detection of viral infection, induction of a rapid immune response, and the appropriate curtailment of that response to prevent immune pathology are key steps in effective host protective immunity. Mice lacking epithelial TGF-β have reduced viral burden and are protected from influenza-induced weight loss, inflammation, and pathological changes. However, contrary to expectation, the key mediators and effector cell populations involved in viral clearance/limiting viral infection were either equivalent or reduced in these mice. Therefore, we explored more closely the mechanisms that are associated with the initial epithelial response to viral infection, namely, the type I and III interferon response.
Firstly, we examined expression of IFNλ protein, finding no significant induction of IFNλ above homeostatic levels in the airways at day 1 post infection in either control or epTGFβKO mice that would reflect the difference in early epithelial damage or viral titer (Figure 5a). In accordance with published work, control-infected mice displayed substantially increased IFNλ levels on day 3 post infection, which by day 6 had reduced to levels similar to baseline.16 However, in epTGFβKO mice, contrary to expectation, the magnitude of peak day 3 response was greatly reduced. We found that IFNλ was the predominant IFN released after infection and was present at much higher levels than type I IFNs (Figure 5a–c), in keeping with published work.16 However, the kinetics of its release were too slow to drive the early immune protection seen in epTGFβKO mice and thus it is unlikely to mediate the early epithelial antiviral response and subsequent protection from influenza-induced pathology observed in mice lacking epithelial TGF-β.
In contrast, IFNβ was substantially increased on day 1 post infection in the airways of epTGFβKO mice compared with both mock-infected and day 1 post infection control mice, with levels remaining elevated on day 3 post infection (Figure 5b). Indeed, in control mice IFNβ levels were not detected until day 3 post infection. We also found that expression of IFNβ in the airways inversely correlated with viral titer on day 1 post infection (P=0.0018, Spearman correlation, r-value −0.6032 and Supplementary Figure 4b). Hence, in mice lacking epithelial TGF-β, IFNβ kinetics in the airways were substantially altered, with a more rapid induction of the peak response and sustained elevation.
However, IFNα, the other key type I interferon family member, did not exhibit the pattern of expression seen with IFNβ (Figure 5c). There was no significant induction of IFNα levels on day 1 post infection between epTGFβKO and control mice. By day 3, IFNα levels were substantially higher in control mice than the epTGFβKO mice, a comparable trend to that seen with IFNλ. These data suggest that IFNβ expression is differentially regulated compared with IFNλ and IFNα. Thereafter, we investigated the cellular source of IFNβ after infection by examining expression patterns of Ifnb1 in both epithelial cells and airway leukocytes. As early as 12 h post infection, Ifnb1 gene expression was increased in epithelial cells from epTGFβKO mice (Figure 5d). This observation correlates with a significant induction of the ISG Oas2 (which activates latent RNAses and aids viral degradation/inhibits viral replication), implying the rapid implementation of an antiviral state in epithelial cells lacking TGF-β expression (Figure 5e). We questioned whether this adoption of an antiviral state was restricted to epithelial cells or whether airway leukocytes (which comprise ∼95% airway macrophages at 12 h post infection, Supplementary Figure 1c) also showed a heighted antiviral state. Expression of Ifnb1 was also increased in airway leukocytes from epTGFβKO mice, although the fold induction relative to infected control was less than that observed in epithelial cells (Figure 5f). Oas2 expression in airway leukocytes also followed a similar pattern (Figure 5g and Supplementary Figure 4c).
On 1 day post infection, both control and epTGFβKO epithelial cells exhibited a marked increase in expression levels of Ifnb1 and Oas2 compared with uninfected mice (Figure 5h,i). However, by this time point there was no difference in Ifnb1 and Oas2 transcript levels between infected control and epTGFβKO epithelial cells; consistent with a rapid process in which the differential Ifnb1 and Oas2 gene expression at 12 h post infection precedes the elevated airway IFNβ protein and reduced pulmonary viral titer observed at 1 day post infection. In contrast, on day 1 post infection, there was a significant increase in Ifnb1 and Oas2 transcript-level epTGFβKO in airway leukocytes compared with control (Figure 5j,k).
This pattern of increased ISG transcript expression in airway leukocytes from mice lacking epithelial TGF-β was observed in other key ISGs- Irf7 (an interferon regulatory transcription factor that positively regulates the type 1 interferon response) and Ifitm3 (inhibits viral entry to host cytoplasm via altered cholesterol homeostasis; Supplementary Figure 4e,f). These early changes in Ifnb1 and ISG gene expression patterns between control and epTGFβKO are consistent with differences in both IFNβ protein in the airways and viral titer at 1 day post infection.
We also examined the early IFN response in RSV-infected mice. In keeping with published studies,17, 18 we find RSV-induced IFNβ peaked on day 1 post infection; there was, however, no difference in IFNβ levels between TGFβKO and control mice (Supplementary Figure 5). Unlike influenza, Ifnb1 mRNA was not induced in epithelial cells on day 1 post RSV infection (Supplementary Figure 5b). Airway leukocytes did exhibit increased Ifnb1 expression on day 1 post RSV infection; however, there was no difference in Ifnb1 levels between TGFβKO and control mice (Supplementary Figure 5c). OAS2 was induced in both epithelial cells and airway leukocytes on day 1 post infection but was not differentially expressed in TGFβKO and control mice (Supplementary Figure 5d,e). Therefore, the kinetics of IFNβ induction in RSV are distinct from influenza. In this context, ablating epithelial TGF-β in RSV infection has little effect on the IFNβ pathways and ultimately on disease pathology.
In order to establish the central role of early induction of IFNβ in protection of TGFβKO mice from influenza infection, we examined the effect of blocking IFNβ during the initial stages of infection. Mice were treated with anti-IFNβ (or isotype control) intranasally 1 day before infection and culled at 1 day post infection (Figure 5l). On day 1 post infection, epTGFβKO mice treated with isotype control had a significant induction of airway IFNβ and this was abrogated in epTGFβKO mice treated with anti-IFNβ (Figure 5m). Concomitant with reduced IFNβ levels, viral titer in epTGFβKO mice treated with anti-IFN-β was significantly increased compared with influenza-infected isotype control-treated mice (Figure 5n). Therefore, the early induction of IFNβ in the airways and generation of a heighted antiviral state is critical to the protection from disease pathology observed in mice lacking epithelial TGF-β.
The pulmonary epithelium has a pivotal role in both the maintenance of immune homeostasis and defense against pathogens in the lung. The detection of viral infection, induction of a rapid immune response, and the appropriate curtailment of that response to prevent immune pathology are key steps in effective host protection, allowing effective viral clearance while minimizing immunopathology.19 We have determined that influenza infection rapidly triggers production and activation of TGF-β from CCSP+ epithelial cells, which acts to constrain early IFNβ production and impair adoption of a pulmonary antiviral state, leading to unrestrained viral replication in the host and increased immunopathology (described schematically Figure 6). Importantly, local antibody blockade of IFNβ reversed the protective effect of epithelial TGF-β ablation on pulmonary viral load, supporting the conclusion that the pro-viral effect of epithelial TGF-β is reliant on negative regulation of IFNβ. We describe this novel function for epithelial TGF-β in vivo, identifying a link between two cytokines with highly location- and context-dependent functions. In this setting, epithelial TGF-β functions as a pro-viral factor by restraining the local antiviral IFNβ response enabling influenza A to temporarily evade host defenses and favor its own replication and survival.
This early TGFβ–IFNβ axis, beginning at the initial site of infection, the bronchial epithelium, critically shapes the outcome of influenza infection. Whereas others have previously reported the activation of TGF-β by influenza,6, 20, 21 we describe for the first time a disease-modifying role for a specific cellular source of TGF-β1 in influenza infection. This relationship between epithelial-derived TGF-β and IFNβ underscores the importance of local and appropriate immune responses in determining the outcomes of infection for the host.
We describe the potential for differential patterns of expression of IFNβ compared with both IFNα and IFNλ, consistent with a report that type I and III IFNs are independently controlled during influenza infection.16 In addition, our data reinforce the concept that, despite overlapping activation pathways, there is a hierarchal relationship between type I and III IFNs, with IFNβ acting before IFNα and IFNλ induction.22, 23 Accordingly, we detected evidence of enhanced epithelial IFNβ activity in epTGFβKO mice as early as 12 h post infection, which quickly spread to an enhanced pulmonary antiviral state detectable in airway leukocytes at 24 h, coincident with decreased lung viral load. In contrast, no enhancement of IFNα levels was detected in BAL of epTGFβKO mice at this time point and IFNλ was undetectable in BAL before 72 h post infection.
The preference for type I vs. type III IFN production by epithelial cells appears organ-specific. Gut epithelial cells exclusively produce type III IFNs,24 whereas pulmonary epithelial cells secrete both type I and III IFNs upon infection.25 Our study supports the theory that, although abundant, both IFNα and IFNλ have a subordinate role in influenza infection, with IFNβ acting as the pivotal inducer/mediator of ISG activation and the antiviral response at the earliest stages of infection.26 Indeed, Wack et al. show that small differences in early interferon induction can have marked influences on host survival.27 Notably, the levels of IFNs we have observed in the current study are comparable to those reported to lead to differential survival outcomes.
In contrast to the protective effects of enhanced IFNβ production in our model, Wack et al. show a pathogenic role for increased type 1 IFN production in the 129 mouse strain after influenza infection. However, although IFNβ levels on 1 day post infection were enhanced in epTGFβKO mice, peak levels did not exceed those of controls at any of the time points tested, indicating that the timing, as well as context of IFNβ release, is critical to its effects during influenza infection.
TGF-β has previously been suggested, by in vitro studies, to act as a modulator of IFNβ during infection.8 Utilizing human epithelial cells infected with rhinovirus Bedke et al. showed that epithelial TGF-β influences IFNβ production.8 Exogenous TGF-β increased viral replication and decreased IFNβ protein secretion, while conversely, blocking TGF-β had reduced viral titer and increased IFNβ levels. We demonstrate for the first time that epithelial TGF-β suppresses IFNβ production in vivo, revealing interactions between the epithelial cytokines and the antiviral response in airway leukocytes that dictate the consequences of mucosal infection.
A very recent study utilizing mice deficient in β6 integrin (and therefore impaired activation of TGF-β) also showed improved survival following influenza infection.21 This protective phenotype could be ablated via the addition of exogenous TGF-β. In this model lower levels of TGF-β at steady state lead to constitutively activated airway macrophages with increased CD11b expression and IFN responsiveness. In contrast, protection in our model was independent of differential expression of activation markers (CD11b/HMCII) on airway macrophages between control and epTGFβKO mice at either homeostasis or after infection. Cumulatively, this suggests that there are differential outcomes to ablating integrin-mediated activation of TGF-β in the lung vs. specific deletion of TGF-β from the club cells of the conducting airway epithelium, which we show to be the source of bioactive TGF-β released into the airway lumen early in influenza infection.
Several previous studies suggest that the timing of TGF-β release and/or activation is absolutely crucial to host outcome with a variety of regimens and interpretations explored. Conflicting results are reported when TGF-β was blocked using pan TGF-β antibodies. One study showed little effect of TGF-β blockade on key markers of influenza pathology,28 whereas in another substantially increased mortality was observed.20 However, in the latter study, pathogenic effects of pan-TGF-β blockade were only reported from 6 days post infection, strongly suggesting a dependence on the role of TGF-β in regulation of T-cell responses28 in contrast to our rapid effect on viral load. Similarly, Furuya et al. show protection of mice from influenza infection after induction of allergic airway diseases to be at least partially TGF-β-mediated.29 However, this protection was independent of viral load and was likely mediated by the suppressive effects of TGF-β on immune cells.
When viewed in the context of these published findings, our data show that ablation of the cell-specific source of TGF-β1 released rapidly upon infection has drastically different consequences to global blockade throughout infection.
We suggest that, in addition to timing, the cellular source of bioactive TGF-β is critical in determining host outcome. It has been proposed that the increase in bioavailable TGF-β after influenza infection arises via direct enzymatic activation of the pool of latent TGF-β6, 20. However, we note a substantial increase in mRNA levels of Tgfb1 in epithelial cells from infected control mice, which is absent in epTGFβKO mice, suggesting that de novo protein synthesis from CCSP+ epithelial cells also occurs. It is pertinent that the induction of Tgfb1 mRNA is restricted to bronchial epithelial cells, which are the early sites of viral attachment and infection. This location-specific effect of TGF-β is underscored by the lack of protection of epTGFβKO from RSV, in which no induction of TGF-β mRNA or bioactive protein was observed rapidly after infection and no enhancement of the early antiviral IFNβ response was observed. This clear distinction between the effects of club cell TGF-β ablation on different respiratory viral infections may be due to tropism of RSV for alveolar rather than bronchial epithelial cells, in which TGF-β expression is intact in our model,3 or the inability of RSV to activate latent TGF-β, which is a feature of multiple influenza strains.20
The hijacking of TGF-β regulatory pathways either through enzymatic activation of host latent TGF-β or via pathogen-derived molecules that mimic TGF-β and induce receptor signaling is an evolutionary adaption utilized by a large number of unrelated pathogens both in in vivo models and human disease.30 In humans, understanding precisely how TGF-β functions in the microenvironmental niche at the site of infection is, naturally, fraught with technical difficulties. However, this study clearly demonstrates a role for early epithelial TGF-β as a local immune regulatory factor with capacity for therapeutic modulation and stresses the importance of the airway epithelium in controlling pulmonary immune response.
Animals, infections, and blocking antibody treatment
Mice on a C57BL/6 background carrying floxed alleles for Tgfb1 (ref. 31) JaTgfb1tm2.1Doe/J, Jackson Laboratory, Bar harbor, ME) were crossed with mice expressing Cre recombinase under the control of the rat CCSP promoter CCSP-rtTA/tetO-Cre.32 Cre expression was induced by a single intraperitoneal injection of 2 mg DOX (Sigma-Aldrich, St Louis, MO) resulting in excision of the Tgfb1 gene (epTGFβKO).3 Littermates carrying two floxed Tgfb1 alleles but lacking either the CCSP-rtTA or tetO-Cre alleles were used as control mice. One week after DOX administration, mice were infected with 1.2 × 104 TICD50 of X31 (a kind gift from Andreas Wack, Crick Institute) in 50 μl phosphate-buffered saline (PBS), or mock-infected with 50 μl PBS. Alternatively, mice were infected with 1 × 106 focus-forming unit RSV in 100 μl (strain A2 (ATCC, Middlesex, UK) was grown in Hep-2 cells as described previously11 or mock-infected with Hep-2 supernatant). In some experiments, mice were administered 100 μg of hamster anti-mouse IFNβ (clone MIB-5E9.1) or isotype control (clone HTK888, both from Biolegend, London, UK) in 100 μl volume intranasally, 24 h before infection. Mice were housed in specific pathogen-free conditions, given food and water ad libitum and used for experiments at 7–12 weeks of age. All procedures were conducted in accordance with the Animals (Scientific Procedures) Act 1986.
Cell and organ processing
BAL was obtained by washing the airways three times with a volume of 400 μl PBS. After centrifugation, the cell pellet was resuspended in 500 μl of complete media (RPMI, 10% fetal calf serum, 2 mM L-glutamine, and 100 U ml−1 penicillin/streptomycin; GIBCO, Life Technologies, Carlsbad, CA) and the supernatant stored at −80 °C for further analysis. The left lobe was finely chopped and digested with 15 mg ml−1 collagenase (Type D; Roche Diagnostics, Burgess Hill, UK) and 25 μg ml−1 DNase (Type 1; Roche Diagnostics) in 4 ml complete media for 1 h at 37 °C with agitation. Lung was then passed through a 70-μm sieve (BD Bioscience), and cells were washed, red blood cells lysed, and the cell pellet resuspended in 1 ml complete media. Airway epithelial cells were obtained by tracheal brushing using 4 mm interdental brushes (TePe, Malmo, Sweden).
Influenza viral titers in the lungs were determined by plaque assay on MDCK cells.33 The inferior lobe was homogenized in 1 ml DMEM containing 100 U ml−1 penicillin/streptomycin (GIBCO, Life Technologies). Briefly, serial dilutions of lung homogenate were incubated with near confluent monolayers of MDCK cells for 1 h at 37 °C; the monolayer was washed and overlaid with plaque overlay media. After 72 h, the overlay media was removed and cells were fixed, stained with crystal violet, and the plaques counted.
Intracellular expression of cytokines was assessed after cells were stimulated with phorbol myristate acetate (PMA) (Sigma-Aldrich)/ionomycin (Emdchemicals) in the presence of Brefeldin A (Sigma-Aldrich) for 3.5 h at 37 °C.
After stimulation, cells were washed and Fc receptors blocked. Extracellular antigens were stained in 5% fetal calf serum/1% bovine serum albumin in PBS for 30 min at 4 °C. Cells were then washed and fixed. Where necessary, cells were permeabilized using Fix/Perm kit (eBioscience, Waltham, MA) and stained for intracellular antigens. All antibodies were obtained from eBioscience except ICOS, CD11c, CD11b, Ly6G, CD64, (Biolegend), and SiglecF (BD Biosciences, Oxford, UK). Biotinylated Db RSV M2187–195 monomers were kindly provided by the NIH Tetramer Core Facility (Atlanta, GA, USA). They were multimerized using PE-conjugated streptavidin (Molecular Probes, Life Technologies) as described on the NIH TCF website (tetramer.yerkes.emory.edu/support). Data were acquired on an LSRII Fortessa (BD Biosciences) and analyzed using FACSDiva (BD Biosciences) and FlowJo (Treestar, La Jolla, CA) software.
The right superior lobe was inflated and fixed in buffered neutral formalin (BNF) before wax imbedding and sectioning. Hematoxylin and eosin-stained lung sections were scored blindly for epithelial damage, inflammation, and lung infiltrates. More than 10 airways and vessels were scored in each lung section. Epithelial damage was scored 0 (normal), 1 (small areas of abnormality), 2 (more than 50% abnormal), 3 (areas of epithelial detachment up to 20%), 4 (areas of epithelial detachment up to 50%), and 5 (more than 50% of the epithelium denuded). Airway and vessel inflammation: 0 (normal), 1 (cell infiltrate/cuffing around the vessel only), 2 (cell infiltrate around the vessel and airways), 3 (double layer of cell infiltrate around the vessel and airways), 4 (multiple layers of cell infiltrate around the vessel and airways), and 5 (large areas of infiltrate).
Immunohistochemistry and scoring of influenza infection
Paraffin-embedded lung sections were subjected to antigen retrieval in boiling citrate buffer and stained with polyclonal goat anti-influenza NP antibody or goat IgG isotype control (Abcam), followed by biotin-conjugated donkey anti-goat secondary antibody (Jackson Immunoresearch). Staining was developed using the Vectastain ABC kit and DAB substrate (Vector Laboratories) as per the manufacturer’s instructions. The extent of bronchiolar NP staining was semiquantitatively analyzed by a blinded investigator, using the following scoring system. The mean of all airways in one section (≥10) was reported for each mouse. 0, no NP staining; 1, 1–25% of epithelial cells in airway stained; 2, 26–50% of epithelial cells in airway stained; 3, 51–75% of epithelial cells in airway stained; 4, 51–75% of epithelial cells in airway stained. An additional +1 score was added where one or more patches of dense staining was observed, giving a maximum score of 5 per bronchiole.
Measurement of cytokines
The right middle lobe was homogenized at 50 mg ml−1 in HBSS (GIBCO) containing one protease inhibitor tablet per 50 ml (Roche Diagnostics) and centrifuged at 800 × g for 20 min at 4 °C. Paired antibodies for murine IFNβ (PBL Assay Science), IFNλ, KC, MCP-1 (R&D Systems, Minneapolis, MN), and IFNα, IL-6, IL-1β (eBioscience) were used in standardized sandwich ELISAs. Bioactive TGF-β in BAL was measured by bioassay as previously described.34
Airway leukocytes and epithelial brushing cells were lysed in RLT buffer and passed through QIAShredder columns, before extracting RNA using the RNeasy Micro Kit (all QIAGEN, Hilden, Germany). Total RNA (≥20 ng) was converted into complementary DNA using the GoSCRIPT Reverse Transcription System (Promega, Madison, WI). Real-time PCRs for mouse genes were performed using Taqman Fast Advanced Master Mix with TaqMan primer/probe sets for murine Oas2, Irf7, Ifitm3, Tgfb1, Scgb1a1 (CCSP), and Ifnb1 plus housekeeping genes Gapdh and Hprt (all Thermo Fisher Scientific, Waltham, MA). Real-time PCR for influenza virus was performed using Fast SYBR Green Master Mix (Thermo Fisher Scientific) with forward and reverse primers for X31 (forward: 5′-TGAGTCTTCTAACCGAGGTC-3′, reverse: 5′-GGTCTTGTCTTTAGCCATTCC-3′) and housekeeping genes Gapdh (forward: 5′-TCCCACTCTTCCACCTTCGA-3′, reverse: 5′-AGTTGGGATAGGGCCTCTCTT-3′) and Actb (forward: 5′-CTAAGGCCAACCGTGAAAAG-3′, reverse: 5′-ACCAGAGGCATACAGGGACA-3′). All real-time PCRs were performed on a Viia7 instrument and threshold cycle (CT) values determined from duplicate reactions using Viia 7 software (Thermo Fisher Scientific). As appropriate, results were expressed as either relative gene expression [1,000/2(CT of target gene−CT of housekeeping genes)] or fold change in relative expression from a control group.
All data were analyzed with Prism 7 (GraphPad). Box and whisker plots depict medians and interquartile ranges. Line graphs show means±s.e.m. Bars on scatter plots show medians and dots indicate data points from individual mice. Non-parametric data were analyzed using Mann–Whitney U-test. Correlations used a Spearman test. Significance was defined as *P<0.05, **P<0.01, and ***P<0.001.
We thank the staff of the Mary Lyon Centre (MLC) at Harwell for performing mouse re-derivation and animal husbandry support, Lorraine Lawrence for histological sectioning, Jessica Rowley and Jane Srivastava of the Imperial College Core Flow Cytometry facility for assistance. We also thank Andreas Wack (The Francis Crick Institute) for his kind gift of influenza virus stocks, and James Harker and Chloe Pyle for guidance with plaque assays and RSV infections. This work was supported by the Welcome Trust (grants 087618/Z/08/Z and 107059/Z/15/Z). WB is supported by a studentship from the MRC & Asthma UK Centre in Allergic Mechanisms of Asthma.
About this article
L.D. wrote the first manuscript draft. L.D. and W.B. performed the majority of the experiments and revised the manuscript. RO performed some of the experiments and assisted in the maintenance of mouse colonies. L.G.G. performed some of the analyses and revised the manuscript. C.M.L. is the principal investigator who conceived the study and edited the manuscript.
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