Figure 8 | Mucosal Immunology

Figure 8

From: Neutrophil pyroptosis mediates pathology of P. aeruginosa lung infection in the absence of the NADPH oxidase NOX2

Figure 8

Neutrophil pyroptosis induced by PAO1 infection is mediated by the Nod-like receptor family, CARD domain-containing 4 (NLRC4) inflammasome. (ac) Bone marrow-derived neutrophils (BMDNs) from Nlrc4−/− and Nlrc4+/+ mice infected with PAO1-GFP (green fluorescent protein). (a) The percentages of pyroptotic BMDNs, including PAO1-GFP, from Nlrc4−/− and Nlrc4+/+ mice were determined 1 h after infection with PAO1-GFP (multiplicity of infection (MOI) 20, 50). (b) The percentages of BMDN uptake from Nlrc4−/− and Nlrc4+/+ mice were determined at 10 and 60 min after infection with PAO1-GFP (MOI 20). (c) Interleukin (IL)-1β levels were detected in the supernatants of BMDNs from Nlrc4−/− and Nlrc4+/+ mice after infection with PAO1 (MOI 20, 50). (df) BMDNs from Nlrp3−/− and Nlrp3+/+ mice infected with PAO1-GFP. (d) The percentages of pyroptotic BMDNs, including PAO1-GFP, from Nlrp3−/− and Nlrp3+/+ mice were determined 1 h after infection with PAO1-GFP (MOI 20, 50). (e) The percentages of BMDN uptake from Nlrp3−/− and Nlrp3+/+ mice were determined at 10 and 60 min postinfection with PAO1-GFP (MOI 20). (f) IL-1β levels were detected in the supernatants of BMDNs from Nlrp3−/− and Nlrp3+/+ mice 1 h after infection with PAO1 (MOI 20, 50). *P<0.05; **P<0.01;***P<0.001.

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