Transcriptional and kinetic analysis of colonic CX3CR1-defined myeloid cells. (a) Quantitative reverse transcription PCR (qRT-PCR) quantitation of CX3CR1, CD163, CD206, TGFβR2, CCR2, interleukin 6 (IL6), and tumor necrosis factor α (TNFα) messenger RNA by fluorescence-activated cell sorted P1, P2, P3, and P4 from colonic mucosa. Results shown are mean expression relative to cyclophilin A using the 2−ΔC(t) method. Means of three independent experiments using pooled cells from 12 mice per experiment. (b, c) CX3CR1+/gfp mice received 1 mg 5-bromo-2-deoxyuridine (BrdU) intraperitoneally and 3, 12, 24, and 48 h later, BrdU uptake was assessed in Ly6Chi blood and bone marrow monocytes (CD11b+Ly6ChiCX3CR1int) (b) and colonic CX3CR1int (P1–P3) and CX3CR1hi (P4) populations (c). Results are means of four mice per group.