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Acute myeloid leukemia

NUP98 is rearranged in 3.8% of pediatric AML forming a clinical and molecular homogenous group with a poor prognosis

Leukemia volume 31, pages 565–572 (2017)Cite this article

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Abstract

Pediatric acute myeloid leukemia (AML) is a rare disease whose prognosis is highly variable according to factors such as chromosomal abnormalities. Recurrent genomic rearrangements are detected in half of pediatric AML by karyotype. NUcleoPorin 98 (NUP98) gene is rearranged with 31 different fusion partner genes. These rearrangements are frequently undetected by conventional cytogenetics, as the NUP98 gene is located at the end of the chromosome 11 short arm (11p15). By screening a series of 574 pediatric AML, we detected a NUP98 rearrangement in 22 cases (3.8%), a frequency similar to CBFB-MYH11 fusion gene (4.0%). The most frequent NUP98 fusion gene partner is NSD1. These cases are homogeneous regarding their biological and clinical characteristics, and associated with bad prognosis only improved by bone marrow transplantation. We detailed the biological characteristics of these AML by exome sequencing which demonstrated few recurrent mutations (FLT3 ITD, WT1, CEBPA, NBPF14, BCR and ODF1). The analysis of the clonal structure in these cases suggests that the mutation order in the NUP98-rearranged pediatric AML begins with the NUP98 rearrangement leading to epigenetic dysregulations then followed by mutations of critical hematopoietic transcription factors and finally, activation of the FLT3 signaling pathway.

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Acknowledgements

The study was initiated by a GFCH collaborative study regarding NUP98 rearrangement in pediatric AML. DE thanks the support of the research in his laboratory: 111 des Arts, Association Laurette Fugain, Société Française des Cancers des Enfants, Ligue Régionale contre le Cancer. Patients samples from the HIMIP collection were obtained after informed consent in accordance with the Declaration of Helsinki. According to the French law, HIMIP collection has been declared to the Ministry of Higher Education and Research (DC 2008-307 collection 1) and obtained a transfer agreement (AC 2008-129) after approbation by ethical committees (Comité de Protection des Personnes Sud-Ouest et Outremer II and APHP ethical committee). Clinical and biological annotations of the samples have been declared to the CNIL (Comité National Informatique et Libertés).

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Author notes

  1. D Penther

    Present address: 20Current address: Department of Haematology, University Hospital of Dijon, France.,

  2. S Lagarde, P Bories and C Puiseux: These two authors contributed equally to this work.

Authors and Affiliations

  1. Department of Haematology, University Hospital of Toulouse, University of Toulouse, Centre of Research on Cancer of Toulouse (CRCT), Toulouse, France

    S Struski, S Lagarde, P Bories, N Prade, E Bart-Delabesse, C Broccardo, I Luquet, M Pasquet & E Delabesse

  2. Groupe Francophone de Cytogénétique Hématologique (GFCH), Paris, France,

    S Struski, W Cuccuini, M-P Pages, A Bidet, C Gervais, M Lafage-Pochitaloff, C Roche-Lestienne, C Barin, D Penther, N Nadal, I Radford-Weiss, M-A Collonge-Rame, B Gaillard, F Mugneret, C Lefebvre & I Luquet

  3. Department of Pediatric Oncology, University Hospital of Toulouse, Toulouse, France

    C Puiseux & M Pasquet

  4. Department of Haematology, University Hospital of Saint-Louis, Paris, France

    W Cuccuini

  5. Department of Haematology, Hospices Civils de Lyon, Lyon, France

    M-P Pages

  6. Department of Haematology, University Hospital of Haut-Leveque, Bordeaux, France

    A Bidet

  7. Department of Haematology, University Hospital of Hautepierre, Strasbourg, France

    C Gervais

  8. Department of Medical Genetic, University Hospital of La Timone, Marseille, France

    M Lafage-Pochitaloff

  9. Department of Medical Genetic, University Hospital Jeanne de Flandre, University of Lille 2, Lille, France

    C Roche-Lestienne

  10. Department of Genetic, University Hospital Bretonneau, Tours, France

    C Barin

  11. Department of Oncology Genetic, Cancer Institute Henri Becquerel, Rouen, France

    D Penther

  12. Department of Haematology, University Hospital of Saint-Étienne, Saint-Etienne, France

    N Nadal

  13. Department of Genetic, University Hospital Necker, Paris, France

    I Radford-Weiss

  14. Department of Genetic, University Hospital Saint-Jacques, Besancon, France

    M-A Collonge-Rame

  15. Department of Haematology, University Hospital Robert Debré, Reims, France

    B Gaillard

  16. Department of Cytogenetic, University Hospital of Dijon, Dijon, France

    F Mugneret

  17. Department of Haematology, Oncology and Immunology, University Hospital of Grenoble, Grenoble, France

    C Lefebvre

  18. Department of Pediatric Oncology, University Hospital of Trousseau, Paris, France

    A Petit & G Leverger

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Correspondence to E Bart-Delabesse.

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Struski, S., Lagarde, S., Bories, P. et al. NUP98 is rearranged in 3.8% of pediatric AML forming a clinical and molecular homogenous group with a poor prognosis. Leukemia 31, 565–572 (2017). https://doi.org/10.1038/leu.2016.267

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