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Functional and genetic aberrations of in vitro-cultured marrow-derived mesenchymal stromal cells of patients with classical Philadelphia-negative myeloproliferative neoplasms

Leukemia volume 28, pages 1742–1745 (2014)Cite this article

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Mesenchymal stromal cells (MSCs) are multipotent progenitor cells that contribute to the structure and function of the bone marrow (BM) niche, controlling homing, self-renewal, differentiation and proliferation of hematopoietic stem/progenitor cells (HSPCs).1 Recently, genetic and functional alterations of MSCs have been reported to occur in patients affected by myelodysplastic syndromes (MDS) and acute leukemias,2, 3, 4 in keeping with the experimental notion that MSC dysfunction can affect relevant functions of leukemic blasts and induce MDS and leukemia in murine models.5

Philadelphia-negative myeloproliferative disorders (Ph-neg MPNs) encompass polycythemia vera (PV), essential thrombocythemia (ET), primary myelofibrosis (PMF) and post-PV or -ET myelofibrosis. Very little is known about BM-derived MSCs of patients with MPNs apart from the absence of the V617FJAK2 mutation,6 the molecular marker of hematopoietic cells in a great proportion of MPN patients.

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Authors and Affiliations

  1. Immunology and Transplantation Laboratory/Cell Factory/Pediatric Hematology/Oncology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy

    M A Avanzini, M Mantelli, E Lenta, D M Ingo, V Achille, A Moretta, R Maccario, G Acquafredda & M Zecca

  2. IRCCS Ospedale Pediatrico Bambino Gesu’, University of Pavia, Roma, Italy

    M E Bernardo & F Locatelli

  3. Department of Molecular Medicine, University of Pavia, Pavia, Italy

    F Novara & O Zuffardi

  4. Center for the Study and Treatment of Myelofibrosis, Research Laboratories of Biotechnology, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy

    V Poletto, L Villani, E Bonetti, R Campanelli, G Fois, P Catarsi, A Aronica, G Barosi & V Rosti

  5. Research Laboratories of Biotechnology, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy

    M Massa

  6. Division of Experimental Medicine, Department of Medicine, Section of Haematology, Imperial College, London, UK

    R P Gale

  7. Cell Therapy Production Unit, Fondazione IRCCS Istituto Neurologico C. Besta, Milano, Italy

    D Lisini

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  1. M A Avanzini
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Avanzini, M., Bernardo, M., Novara, F. et al. Functional and genetic aberrations of in vitro-cultured marrow-derived mesenchymal stromal cells of patients with classical Philadelphia-negative myeloproliferative neoplasms. Leukemia 28, 1742–1745 (2014). https://doi.org/10.1038/leu.2014.97

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