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Myelodysplasias

Clonal diversity of recurrently mutated genes in myelodysplastic syndromes

Leukemia volume 27, pages 1275–1282 (2013)Cite this article

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Abstract

Recent studies suggest that most cases of myelodysplastic syndrome (MDS) are clonally heterogeneous, with a founding clone and multiple subclones. It is not known whether specific gene mutations typically occur in founding clones or subclones. We screened a panel of 94 candidate genes in a cohort of 157 patients with MDS or secondary acute myeloid leukemia (sAML). This included 150 cases with samples obtained at MDS diagnosis and 15 cases with samples obtained at sAML transformation (8 were also analyzed at the MDS stage). We performed whole-genome sequencing (WGS) to define the clonal architecture in eight sAML genomes and identified the range of variant allele frequencies (VAFs) for founding clone mutations. At least one mutation or cytogenetic abnormality was detected in 83% of the 150 MDS patients and 17 genes were significantly mutated (false discovery rate ⩽0.05). Individual genes and patient samples displayed a wide range of VAFs for recurrently mutated genes, indicating that no single gene is exclusively mutated in the founding clone. The VAFs of recurrently mutated genes did not fully recapitulate the clonal architecture defined by WGS, suggesting that comprehensive sequencing may be required to accurately assess the clonal status of recurrently mutated genes in MDS.

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Acknowledgements

This work was supported by NIH grants R01HL082973 (Graubert), RC2HL102927 (Graubert), U54HG003079 (Wilson), P01CA101937 (Ley), U01HG006517 (Ding), and a Howard Hughes Medical Institute Physician-Scientist Early Career Award (Walter). Technical assistance was provided by the Alvin J. Siteman Cancer Center Tissue Procurement Core, which is supported by an NCI Cancer Center Support Grant P30CA91842. Variant calls from WGS data have been deposited in dbGAP under accession number phs000159.v5.p2.

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Author notes

  1. M J Walter, D Shen and J Shao: These authors contributed equally to this work.

Authors and Affiliations

  1. Division of Oncology, Department of Internal Medicine, Washington University School of Medicine, St Louis, MO, USA

    M J Walter, J Shao, L Ding, B S White, K Elliot, S Heath, M Grillot, P Westervelt, D C Link, J F DiPersio, T J Ley & T A Graubert

  2. Department of Genetics, Washington University School of Medicine, St Louis, MO, USA

    M J Walter, L Ding, E Mardis, T J Ley & R K Wilson

  3. Siteman Cancer Center, Washington University School of Medicine, St Louis, MO, USA

    M J Walter, L Ding, P Westervelt, D C Link, J F DiPersio, T J Ley, R K Wilson & T A Graubert

  4. The Genome Institute, Washington University School of Medicine, St Louis, MO, USA

    D Shen, L Ding, B S White, C Kandoth, C A Miller, B Niu, M D McLellan, N D Dees, R Fulton, E Mardis, T J Ley & R K Wilson

  5. Department of Molecular Microbiology, Washington University School of Medicine, St Louis, MO, USA

    R K Wilson

  6. Department of Pathology and Immunology, Washington University School of Medicine, St Louis, MO, USA

    T A Graubert

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  1. M J Walter
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  2. D Shen
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Correspondence to T A Graubert.

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Walter, M., Shen, D., Shao, J. et al. Clonal diversity of recurrently mutated genes in myelodysplastic syndromes. Leukemia 27, 1275–1282 (2013). https://doi.org/10.1038/leu.2013.58

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