Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter to the Editor
  • Published:

CXCR4 expression in CD34+ cells and unit predominance after double umbilical cord blood transplantation

Leukemia volume 27, pages 1181–1183 (2013)Cite this article

Subjects

Hematopoietic stem cell (HSC) biology studies have shown that several adhesion molecules, such as CXCR4, VLA4, VLA5, CD44, Cd11a and L-selectin, are involved in self-renewal, trafficking, survival and adhesion of HSC to their niches.1 Similarly to adult HSC, umbilical cord blood (UCB) HSC have been shown to highly express CXCR4 and other adhesion molecules involved in engraftment and trafficking.2, 3

The applicability of UCB transplantation had been historically limited by the small cell number available from a single unit.4 Over the past decade, the utilization of double UCB (dUCB) unit grafts was shown to, at least in part, overcome the cell dose limitation and has been adopted by several institutions.5, 6 Our group7 and others5, 8 have shown that despite the infusion of two UCB units, in the vast majority of cases only a single unit will predominate and provide long-term lympho-hematopoietic recovery. Available data shows that UCB unit predominance is influenced by the T-cell content an interaction between T cells from both donor units.5, 7 Identifying additional factors that determine the long-term predominant unit after dUCB transplantation will allow us to further refine UCB graft selection, and potentially improve outcomes. Thus, we studied whether the expression of CXCR4 on UCB CD34+ cells and different CD34+ cells subsets were also involved in unit predominance after dUCB transplantation.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1

References

  1. Lapidot T, Dar A, Kollet O . How do stem cells find their way home? Blood 2005; 106: 1901–1910.

    Article  CAS  Google Scholar 

  2. Ohno N, Kajiume T, Sera Y, Sato T, Kobayashi M . Short-term culture of umbilical cord blood-derived CD34 cells enhances engraftment into NOD/SCID mice through increased CXCR4 expression. Stem Cells Dev 2009; 18: 1221–1226.

    Article  CAS  Google Scholar 

  3. Timeus F, Crescenzio N, Basso G, Ramenghi U, Saracco P, Gabutti V . Cell adhesion molecule expression in cord blood CD34+ cells. Stem Cells 1998; 16: 120–126.

    Article  CAS  Google Scholar 

  4. Brunstein CG, Barker JN, Weisdorf DJ, DeFor TE, Miller JS, Blazar BR et al. Umbilical cord blood transplantation after nonmyeloablative conditioning: impact on transplantation outcomes in 110 adults with hematologic disease. Blood 2007; 110: 3064–3070.

    Article  CAS  Google Scholar 

  5. Gutman JA, Turtle CJ, Manley TJ, Heimfeld S, Bernstein ID, Riddell SR et al. Single-unit dominance after double-unit umbilical cord blood transplantation coincides with a specific CD8+ T-cell response against the nonengrafted unit. Blood 2010; 115: 757–765.

    Article  CAS  Google Scholar 

  6. Scaradavou A, Smith KM, Hawke R, Schaible A, Abboud M, Kernan NA et al. Cord blood units with low CD34+ cell viability have a low probability of engraftment after double unit transplantation. Biol Blood Marrow Transplant 2010; 16: 500–508.

    Article  Google Scholar 

  7. Ramirez P, Wagner JE, Defor TE, Blazar BR, Verneris MR, Miller JS et al. Factors predicting single-unit predominance after double umbilical cord blood transplantation. Bone Marrow Transplant 2012; 47: 799–803.

    Article  CAS  Google Scholar 

  8. Kanda J, Rizzieri DA, Gasparetto C, Long GD, Chute JP, Sullivan KM et al. Adult dual umbilical cord blood transplantation using myeloablative total body irradiation (1350 cGy) and fludarabine conditioning. Biol Blood Marrow Transplant 2011; 17: 867–874.

    Article  Google Scholar 

  9. Barker JN, Weisdorf DJ, DeFor TE, Blazar BR, McGlave PB, Miller JS et al. Transplantation of 2 partially HLA-matched umbilical cord blood units to enhance engraftment in adults with hematologic malignancy. Blood 2005; 105: 1343–1347.

    Article  CAS  Google Scholar 

  10. Timeus F, Crescenzio N, Saracco P, Doria A, Fazio L, Albiani R et al. Recovery of cord blood hematopoietic progenitors after successive freezing and thawing procedures. Haematologica 2003; 88: 74–79.

    PubMed  Google Scholar 

  11. Broxmeyer HE . Chemokines in hematopoiesis. Curr Opin Hematol 2008; 15: 49–58.

    Article  CAS  Google Scholar 

  12. Ratajczak J, Reca R, Kucia M, Majka M, Allendorf DJ, Baran JT et al. Mobilization studies in mice deficient in either C3 or C3a receptor (C3aR) reveal a novel role for complement in retention of hematopoietic stem/progenitor cells in bone marrow. Blood 2004; 103: 2071–2078.

    Article  CAS  Google Scholar 

  13. Hoggatt J, Singh P, Sampath J, Pelus LM . Prostaglandin E2 enhances hematopoietic stem cell homing, survival, and proliferation. Blood 2009; 113: 5444–5455.

    Article  CAS  Google Scholar 

  14. Cutler CS, Shoemaker D, Ballen KK, Robbins D, Desponts C, Kao GS et al. FT1050 (16,16-dimethyl Prostaglandin E2)-enhanced umbilical cord blood accelerates hematopoietic engraftment after reduced intensity conditioning and double umbilical cord blood transplantation. ASH Annu Meet Abstr 2011; 118: 653.

    Google Scholar 

  15. Brunstein CG, McKenna DH, DeFor TE, Sumstad D, Ratajczak M, Laughlin MJ et al. Priming of hematopoietic progenitor cells (HPC) with complement fragment 3A (C3A) to promote homing of umbilical cord blood (UCB): safety profile. Biol Blood Marrow Transplant 2012; 18: S210.

    Article  Google Scholar 

Download references

Acknowledgements

This work was supported in part by grants from the National Cancer Institute CA65493 (JEW, JSM, CGB), the Children’s Cancer Research Fund (JEW, TED), the American Cancer Society Audrey Meyer Mars International Fellowship in Clinical Oncology (PR) and Pontificia Universidad Católica de Chile (PR), American Society of Blood and Marrow Transplantation Robert A Good New Investigator Award (CGB), and Leukemia and Lymphoma Society Scholar in Clinical Research Award (CGB).

Author information

Authors and Affiliations

  1. Blood and Marrow Transplantation Program, University of Minnesota Medical Center, Minneapolis, MN, USA

    P Ramirez, J E Wagner, T E DeFor, C R Eide, J S Miller, D J Weisdorf & C G Brunstein

Authors
  1. P Ramirez
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J E Wagner
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. T E DeFor
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. C R Eide
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. J S Miller
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. D J Weisdorf
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. C G Brunstein
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to C G Brunstein.

Ethics declarations

Competing interests

The authors declare no conflict of interest.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ramirez, P., Wagner, J., DeFor, T. et al. CXCR4 expression in CD34+ cells and unit predominance after double umbilical cord blood transplantation. Leukemia 27, 1181–1183 (2013). https://doi.org/10.1038/leu.2012.261

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/leu.2012.261

Search

Advanced search

Quick links