Skip to main content

Thank you for visiting You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Lentiviral marking of patient-derived acute lymphoblastic leukaemic cells allows in vivo tracking of disease progression

A Corrigendum to this article was published on 07 August 2013

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Rent or buy this article

Get just this article for as long as you need it


Prices may be subject to local taxes which are calculated during checkout

Figure 1
Figure 2


  1. Meacham CE, Ho EE, Dubrovsky E, Gertler FB, Hemann MT . In vivo RNAi screening identifies regulators of actin dynamics as key determinants of lymphoma progression. Nat Genet 2009; 41: 1133–1137.

    Article  CAS  Google Scholar 

  2. Shultz LD, Lyons BL, Burzenski LM, Gott B, Chen X, Chaleff S et al. Human lymphoid and myeloid cell development in NOD/LtSz-scid IL2R gamma null mice engrafted with mobilized human hemopoietic stem cells. J Immunol 2005; 174: 6477–6489.

    Article  CAS  Google Scholar 

  3. Hong D, Gupta R, Ancliff P, Atzberger A, Brown J, Soneji S et al. Initiating and cancer-propagating cells in TEL-AML1-associated childhood leukemia. Science 2008; 319: 336–339.

    Article  CAS  Google Scholar 

  4. Kong Y, Yoshida S, Saito Y, Doi T, Nagatoshi Y, Fukata M et al. CD34+CD38+CD19+ as well as CD34+CD38-CD19+ cells are leukemia-initiating cells with self-renewal capacity in human B-precursor ALL. Leukemia 2008; 22: 1207–1213.

    Article  CAS  Google Scholar 

  5. le Viseur C, Hotfilder M, Bomken S, Wilson K, Rottgers S, Schrauder A et al. In childhood acute lymphoblastic leukemia, blasts at different stages of immunophenotypic maturation have stem cell properties. Cancer Cell 2008; 14: 47–58.

    Article  CAS  Google Scholar 

  6. Martinez Soria N, Tussiwand R, Ziegler P, Manz MG, Heidenreich O . Transient depletion of RUNX1/RUNX1T1 by RNA interference delays tumour formation in vivo. Leukemia 2009; 23: 188–190.

    Article  CAS  Google Scholar 

  7. Greil J, Gramatzki M, Burger R, Marschalek R, Peltner M, Trautmann U et al. The acute lymphoblastic leukaemia cell line SEM with t(4;11) chromosomal rearrangement is biphenotypic and responsive to interleukin-7. Br J Haematol 1994; 86: 275–283.

    Article  CAS  Google Scholar 

  8. Kustikova OS, Modlich U, Fehse B . Retroviral insertion site analysis in dominant haematopoietic clones. Methods Mol Biol 2009; 506: 373–390.

    Article  CAS  Google Scholar 

  9. Schmidt M, Hoffmann G, Wissler M, Lemke N, Mussig A, Glimm H et al. Detection and direct genomic sequencing of multiple rare unknown flanking DNA in highly complex samples. Hum Gene Ther 2001; 12: 743–749.

    Article  CAS  Google Scholar 

  10. Bonnet D, Dick JE . Human acute myeloid leukemia is organized as a hierarchy that originates from a primitive hematopoietic cell. Nat Med 1997; 3: 730–737.

    Article  CAS  Google Scholar 

  11. Hope KJ, Jin L, Dick JE . Acute myeloid leukemia originates from a hierarchy of leukemic stem cell classes that differ in self-renewal capacity. Nat Immunol 2004; 5: 738–743.

    Article  CAS  Google Scholar 

  12. Lapidot T, Sirard C, Vormoor J, Murdoch B, Hoang T, Caceres-Cortes J et al. A cell initiating human acute myeloid leukaemia after transplantation into SCID mice. Nature 1994; 367: 645–648.

    Article  CAS  Google Scholar 

  13. Rehe K, Wilson K, Bomken S, McNeill H, Stanulla M, Den Boer ML et al. In acute lymphoblastic leukaemia, stemness is frequent and ubiquitous. Blood 2010; 116, : Abstract 92.

  14. Morisot S, Wayne AS, Bohana-Kashtan O, Kaplan IM, Gocke CD, Hildreth R et al. High frequencies of leukemia stem cells in poor-outcome childhood precursor-B acute lymphoblastic leukemias. Leukemia 2010; 24: 1859–1866.

    Article  CAS  Google Scholar 

Download references


We thank Christopher Baum and Olga Kustikova for their help with the ligation-mediated PCR and Martin Stanulla and Martin Schrappe for the provision of sample L4951. SB is funded by a Medical Research Council (MRC) Clinical Research Training Fellowship. The IVIS Spectrum was funded by a grant from the Wellcome Trust, grant 087961. Additional funding came from Leukaemia Lymphoma Research (LLR) and core infrastructure support from the North of England Children′s Cancer Research Fund (NECCR).

Author information

Authors and Affiliations


Corresponding author

Correspondence to O Heidenreich.

Additional information

Supplementary Information accompanies the paper on the Leukemia website

Supplementary information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Bomken, S., Buechler, L., Rehe, K. et al. Lentiviral marking of patient-derived acute lymphoblastic leukaemic cells allows in vivo tracking of disease progression. Leukemia 27, 718–721 (2013).

Download citation

  • Published:

  • Issue Date:

  • DOI:

This article is cited by


Quick links