CD38 localizes in the cell adhesion sites. CLL cells were plated onto either VCAM-1-coated or FN-coated (H89/CS-1 FN fragment) coverslips and allowed to adhere for 30 min at 37 °C. Adherent cells were incubated with the anti-CD49d mAb, next reacted with TRITC-labeled GαMIg (red) and finally stained with CD38-FITC (green). Coverslips were mounted on slides and analyzed. The panels on the right show the merging of the two images and the differential interference contrast field. Images refer to two representative cases (CLL #3, upper panel; CLL#4, lower panel). Original magnification × 60. Scale bar, 5 μm.