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Lymphoma

Consequences of heat shock protein 72 (Hsp72) expression and activity on stress-induced apoptosis in CD30+ NPM–ALK+ anaplastic large-cell lymphomas

Leukemia volume 26, pages 1375–1382 (2012)Cite this article

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Abstract

Understanding the mechanisms that control stress-induced apoptosis is critical to explain how tumours respond to treatment, as cancer cells frequently escape drug toxicity by regulating stress response through heat shock protein (HSP) expression. The overexpression of Hsp72, in particular, results in increased incidence of cell transformation, and correlates with poor prognosis in a wide range of cancers. We have shown that Hsp72 assists folding of oncogenic NPM–ALK kinase in anaplastic large-cell lymphomas (ALCLs), but its role in the maintenance of the malignant phenotype remains uncertain. Therefore, we assessed Hsp72 expression in ALCLs, investigating more in detail the mechanisms that regulate its status and activity. We found that Hsp72 is unique among the HSPs involved in tumourigenesis to be overexpressed in ALK+ tumours and cell lines and to be induced by stress. Different from other HSPs, Hsp72 prevents cell injury, Bax activation and death by apoptosis in ALK+ cells, acting both upstream and downstream of mitochondria. Conversely, Hsp72 is underexpressed in ALK− ALCL cells, and it is unable to protect cells from apoptosis under stress. Moreover, when Hsp72 expression is reduced following NPM–ALK inhibition, lymphoma cells undergo apoptosis, demonstrating the importance of Hsp72 in regulating ALCL stress response and drug sensitivity.

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References

  1. Arya R, Mallik M, Lakhotia SC . Heat shock genes - integrating cell survival and death. J Biosci 2007; 32: 595–610.

    Article  CAS  PubMed  Google Scholar 

  2. Galluzzi L, Giordanetto F, Kroemer G . Targeting HSP70 for cancer therapy. Mol Cell 2009; 36: 176–177.

    Article  CAS  PubMed  Google Scholar 

  3. Awasthi N, Wagner BJ . Upregulation of heat shock protein expression by proteasome inhibition: an antiapoptotic mechanism in the lens. Invest Ophthalmol Vis Sci 2005; 46: 2082–2091.

    Article  PubMed  Google Scholar 

  4. Kampinga HH, Craig EA . The HSP70 chaperone machinery: J proteins as drivers of functional specificity. Nat Rev Mol Cell Biol 2010; 11: 579–592.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Abravaya K, Myers MP, Murphy SP, Morimoto RI . The human heat shock protein hsp70 interacts with HSF, the transcription factor that regulates heat shock gene expression. Genes Dev 1992; 6: 1153–1164.

    Article  CAS  PubMed  Google Scholar 

  6. Bonvini P, Gastaldi T, Falini B, Rosolen A . Nucleophosmin-anaplastic lymphoma kinase (NPM-ALK), a novel Hsp90-client tyrosine kinase: down-regulation of NPM-ALK expression and tyrosine phosphorylation in ALK(+) CD30(+) lymphoma cells by the Hsp90 antagonist 17-allylamino,17-demethoxygeldanamycin. Cancer Res 2002; 62: 1559–1566.

    CAS  PubMed  Google Scholar 

  7. Lagarrigue F, Dupuis-Coronas S, Ramel D, Delsol G, Tronchere H, Payrastre B et al. Matrix metalloproteinase-9 is upregulated in nucleophosmin-anaplastic lymphoma kinase-positive anaplastic lymphomas and activated at the cell surface by the chaperone heat shock protein 90 to promote cell invasion. Cancer Res 2010; 70: 6978–6987.

    Article  CAS  PubMed  Google Scholar 

  8. Kinney MC, Higgins RA, Medina EA . Anaplastic large cell lymphoma: twenty-five years of discovery. Arch Pathol Lab Med 2011; 135: 19–43.

    PubMed  Google Scholar 

  9. Bonvini P, Zorzi E, Basso G, Rosolen A . Bortezomib-mediated 26S proteasome inhibition causes cell-cycle arrest and induces apoptosis in CD-30+ anaplastic large cell lymphoma. Leukemia 2007; 21: 838–842.

    Article  CAS  PubMed  Google Scholar 

  10. Bonvini P, Zorzi E, Mussolin L, Monaco G, Pigazzi M, Basso G et al. The effect of the cyclin-dependent kinase inhibitor flavopiridol on anaplastic large cell lymphoma cells and relationship with NPM-ALK kinase expression and activity. Haematologica 2009; 94: 944–955.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Mussolin L, Pillon M, d’Amore ES, Santoro N, Lombardi A, Fagioli F et al. Prevalence and clinical implications of bone marrow involvement in pediatric anaplastic large cell lymphoma. Leukemia 2005; 19: 1643–1647.

    Article  CAS  PubMed  Google Scholar 

  12. Ghisi M, Corradin A, Basso K, Frasson C, Serafin V, Mukherjee S et al. Modulation of microRNA expression in human T-cell development: targeting of Notch3 by miR-150. Blood 2011; 117: 7053–7062.

    Article  CAS  PubMed  Google Scholar 

  13. Joly AL, Wettstein G, Mignot G, Ghiringhelli F, Garrido C . Dual role of heat shock proteins as regulators of apoptosis and innate immunity. J Innate Immun 2010; 2: 238–247.

    Article  CAS  PubMed  Google Scholar 

  14. Zou J, Guo Y, Guettouche T, Smith DF, Voellmy R . Repression of heat shock transcription factor HSF1 activation by HSP90 (HSP90 complex) that forms a stress-sensitive complex with HSF1. Cell 1998; 94: 471–480.

    Article  CAS  PubMed  Google Scholar 

  15. Didelot C, Schmitt E, Brunet M, Maingret L, Parcellier A, Garrido C . Heat shock proteins: endogenous modulators of apoptotic cell death. Handb Exp Pharmacol 2006; 172: 171–198.

    Article  CAS  Google Scholar 

  16. Jego G, Hazoume A, Seigneuric R, Garrido C . Targeting heat shock proteins in cancer. Cancer Lett 2010; e-pub ahead of print 13 November 2010; doi:10.1016/j.canlet.2010.10.014.

    Article  CAS  PubMed  Google Scholar 

  17. Pirkkala L, Nykanen P, Sistonen L . Roles of the heat shock transcription factors in regulation of the heat shock response and beyond. FASEB J 2001; 15: 1118–1131.

    Article  CAS  PubMed  Google Scholar 

  18. Dudeja V, Mujumdar N, Phillips P, Chugh R, Borja-Cacho D, Dawra RK et al. Heat shock protein 70 inhibits apoptosis in cancer cells through simultaneous and independent mechanisms. Gastroenterology 2009; 136: 1772–1782.

    Article  CAS  PubMed  Google Scholar 

  19. Bai RY, Ouyang T, Miething C, Morris SW, Peschel C, Duyster J . Nucleophosmin-anaplastic lymphoma kinase associated with anaplastic large-cell lymphoma activates the phosphatidylinositol 3-kinase/Akt antiapoptotic signaling pathway. Blood 2000; 96: 4319–4327.

    CAS  PubMed  Google Scholar 

  20. Chiarle R, Gong JZ, Guasparri I, Pesci A, Cai J, Liu J et al. NPM-ALK transgenic mice spontaneously develop T-cell lymphomas and plasma cell tumors. Blood 2003; 101: 1919–1927.

    Article  CAS  PubMed  Google Scholar 

  21. Chiarle R, Simmons WJ, Cai H, Dhall G, Zamo A, Raz R et al. Stat3 is required for ALK-mediated lymphomagenesis and provides a possible therapeutic target. Nat Med 2005; 11: 623–629.

    Article  CAS  PubMed  Google Scholar 

  22. Ladanyi M . The NPM/ALK gene fusion in the pathogenesis of anaplastic large cell lymphoma. Cancer Surv 1997; 30: 59–75.

    CAS  PubMed  Google Scholar 

  23. Mosse YP, Wood A, Maris JM . Inhibition of ALK signaling for cancer therapy. Clin Cancer Res 2009; 15: 5609–5614.

    Article  CAS  PubMed  Google Scholar 

  24. Palmer RH, Vernersson E, Grabbe C, Hallberg B . Anaplastic lymphoma kinase: signalling in development and disease. Biochem J 2009; 420: 345–361.

    Article  CAS  PubMed  Google Scholar 

  25. Piva R, Chiarle R, Manazza AD, Taulli R, Simmons W, Ambrogio C et al. Ablation of oncogenic ALK is a viable therapeutic approach for anaplastic large-cell lymphomas. Blood 2006; 107: 689–697.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  26. Solimini NL, Luo J, Elledge SJ . Non-oncogene addiction and the stress phenotype of cancer cells. Cell 2007; 130: 986–988.

    Article  CAS  PubMed  Google Scholar 

  27. Tyedmers J, Mogk A, Bukau B . Cellular strategies for controlling protein aggregation. Nat Rev Mol Cell Biol 2010; 11: 777–788.

    Article  CAS  PubMed  Google Scholar 

  28. Young JC . Mechanisms of the Hsp70 chaperone system. Biochem Cell Biol 2010; 88: 291–300.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  29. Morimoto RI, Santoro MG . Stress-inducible responses and heat shock proteins: new pharmacologic targets for cytoprotection. Nat Biotechnol 1998; 16: 833–838.

    Article  CAS  PubMed  Google Scholar 

  30. Garrido C, Schmitt E, Cande C, Vahsen N, Parcellier A, Kroemer G . HSP27 and HSP70: potentially oncogenic apoptosis inhibitors. Cell Cycle 2003; 2: 579–584.

    Article  CAS  PubMed  Google Scholar 

  31. Diakos C, Krapf G, Gerner C, Inthal A, Lemberger C, Ban J et al. RNAi-mediated silencing of TEL/AML1 reveals a heat-shock protein- and survivin-dependent mechanism for survival. Blood 2007; 109: 2607–2610.

    Article  CAS  PubMed  Google Scholar 

  32. Guo F, Sigua C, Bali P, George P, Fiskus W, Scuto A et al. Mechanistic role of heat shock protein 70 in Bcr-Abl-mediated resistance to apoptosis in human acute leukemia cells. Blood 2005; 105: 1246–1255.

    Article  CAS  PubMed  Google Scholar 

  33. Pocaly M, Lagarde V, Etienne G, Ribeil JA, Claverol S, Bonneu M et al. Overexpression of the heat-shock protein 70 is associated to imatinib resistance in chronic myeloid leukemia. Leukemia 2007; 21: 93–101.

    Article  CAS  PubMed  Google Scholar 

  34. Yao Q, Nishiuchi R, Li Q, Kumar AR, Hudson WA, Kersey JH . FLT3 expressing leukemias are selectively sensitive to inhibitors of the molecular chaperone heat shock protein 90 through destabilization of signal transduction-associated kinases. Clin Cancer Res 2003; 9: 4483–4493.

    CAS  PubMed  Google Scholar 

  35. Gotoh T, Terada K, Oyadomari S, Mori M . hsp70-DnaJ chaperone pair prevents nitric oxide- and CHOP-induced apoptosis by inhibiting translocation of Bax to mitochondria. Cell Death Differ 2004; 11: 390–402.

    Article  CAS  PubMed  Google Scholar 

  36. Stankiewicz AR, Lachapelle G, Foo CP, Radicioni SM, Mosser DD . Hsp70 inhibits heat-induced apoptosis upstream of mitochondria by preventing Bax translocation. J Biol Chem 2005; 280: 38729–38739.

    Article  CAS  PubMed  Google Scholar 

  37. Stankiewicz AR, Livingstone AM, Mohseni N, Mosser DD . Regulation of heat-induced apoptosis by Mcl-1 degradation and its inhibition by Hsp70. Cell Death Differ 2009; 16: 638–647.

    Article  CAS  PubMed  Google Scholar 

  38. Beere HM, Wolf BB, Cain K, Mosser DD, Mahboubi A, Kuwana T et al. Heat-shock protein 70 inhibits apoptosis by preventing recruitment of procaspase-9 to the Apaf-1 apoptosome. Nat Cell Biol 2000; 2: 469–475.

    Article  CAS  PubMed  Google Scholar 

  39. Nylandsted J, Brand K, Jaattela M . Heat shock protein 70 is required for the survival of cancer cells. Ann NY Acad Sci 2000; 926: 122–125.

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

We thank Dr G Franceschetto for the statistical analysis; A Cabrelle (Venetian Institute of Molecular Medicine, Padova, Italy) for performing FACS analysis; and E Tosato and A Molin for their technical expertise and support. This work was supported by Fondazione Città della Speranza and MIUR (Ministero Istruzione Università e Ricerca).

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  1. P Bonvini and E Zorzi: These authors contributed equally to this work.

Authors and Affiliations

  1. Pediatric Onco-Hematology Clinic, University Hospital Padova, Padova, Italy

    P Bonvini, E Zorzi, L Mussolin, M Pillon, M Peron & A Rosolen

  2. Fondazione Città della Speranza, Malo, Vicenza, Italy

    P Bonvini & L Mussolin

  3. Department of Biological Sciences, CRIBI Biotechnology Center, University of Padova, Padova, Italy

    C Romualdi

  4. Department of Pathology, San Bortolo Hospital, Vicenza, Italy

    E S G d'Amore

  5. CRCT, INSERM UMR1037, Laboratoire d’Anatomie Pathologique, CHU Purpan Université Paul Sabatier, Toulouse, France

    L Lamant

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Correspondence to P Bonvini.

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Bonvini, P., Zorzi, E., Mussolin, L. et al. Consequences of heat shock protein 72 (Hsp72) expression and activity on stress-induced apoptosis in CD30+ NPM–ALK+ anaplastic large-cell lymphomas. Leukemia 26, 1375–1382 (2012). https://doi.org/10.1038/leu.2011.367

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