Table 1 Serial transplantation of primary human B-ALL CD34+CD38+CD19+ and CD34+CD38-CD19+ cells

From: CD34+CD38+CD19+ as well as CD34+CD38−CD19+ cells are leukemia-initiating cells with self-renewal capacity in human B-precursor ALL

Patient ID Transplant round Graft cell type Graft cell dose Survival post- transplant (weeks) % BM hCD45+CD19+ % spleen hCD45+CD19+ % PB hCD45+CD19+
(a)
 1 Primary 34+38+19+ 6.5E+04 13 63.7 27.8 ND
   34+38+19+ 6.5E+04 13 74.8 33.4 ND
   34+38+19+ 1.0E+05 15 93.5 46.9 78.7
  Secondary 34+38+19+ 8.0E+04 12 97.8 65.3 92.0
   34+38+19+ 3.0E+04 11 ND ND 95.0
   34+ 2.0E+05 8 ND ND 89.9
   34+ 2.0E+05 9 95.7 90.2 42.8
 2 Primary 34+38+19+ 5.0E+03 6 99.9 ND 69.4
   34+38+19+ 5.0E+04 5 ND ND 73.8
   34+38+19+ 5.0E+04 5 99.9 99.4 82.3
   34+38+19+ 8.0E+04 5 54.9 10.8 10.7
  Secondary 34+38+19+ 3.0E+04 7 86.3 87.4 60.3
   34+ 1.0E+04 5 99.6 99.5 92.2
   34+ 1.0E+05 4 98.4 89.1 ND
   34+ 4.0E+06 4 90.3 83.3 ND
  Tertiary 34+ 1.0E+05 5 ND ND 73.4
   34+ 1.0E+05 4 94.9 73.2 75.3
   34+ 1.0E+06 4 93.9 72.2 94.8
   34+ 1.0E+06 4 96.1 87.4 94.0
 3 Primary 34+38+19+ 2.5E+04 10 98.1 87.9 72.3
   34+38+19+ 2.5E+05 7 98.8 52.7 61.2
   34+38+19+ 5.0E+05 11 99.1 77.1 87.7
   34+38+19+ 1.0E+04 13 97.9 44.4 23.3
   34+38−19+ 6.5E+04 10 90.0 ND 70.0
  Secondary 34+38+19+ 1.0E+05 6 91.2 ND ND
   34+38+19+ 1.0E+06 4 ND 94.7 ND
   34+ 1.0E+05 9 72.8 ND 71.5
  Tertiary 34+ 1.0E+05 9 ND ND 98.2
   34+ 1.0E+05 9 ND ND 90.3
   34+ 1.0E+06 7 99.7 96.1 91.5
   34+ 1.0E+06 8 98.7 99.9 56.9
(b)
 1 Primary 34+38−19+ 6.5E+04 12 52.4 37.2 ND
   34+38−19+ 1.0E+05 13 90.3 42.2 76.0
  Secondary 34+ 2.8E+05 4 97.6 99.3 ND
   34+ 2.8E+05 4 95.2 99.9 ND
   34+ 2.8E+05 4 98.8 ND ND
   34+ 2.8E+06 4 98.2 99.3 ND
   34+ 2.8E+06 4 98.5 99.6 ND
   34+ 2.8E+06 4 99.5 99.1 ND
 2 Primary 34+38−19+ 4.0E+04 5 6.0 1.8 1.1
   34+38−19+ 4.0E+04 5 7.9 4.8 1.1
   34+38−19+ 5.0E+03 7 98.7 77.6 85.6
   34+38−19+ 5.0E+03 7 ND ND 85.7
  Secondary 34+ 2.0E+05 4 13.8 ND ND
   34+ 2.0E+05 4 7.6 36.0 ND
   34+ 2.0E+05 4 11.0 41.0 ND
   34+ 2.0E+05 8 87.8 49.2 47.0
 3 Primary 34+38−19+ 6.5E+04 10 90.0 ND 70.0
  1. Abbreviations: ALL, acute lymphocytic leukemia; BM, bone marrow; ND, not determined; PB, peripheral blood.
  2. PB, BM and spleen engraftment levels in primary, secondary and tertiary recipients from: (a) primary recipients of CD34+CD38+CD19+ B-ALL cells and (b) primary recipients of CD34+CD38-CD19+ human B-ALL cells.