Figure 5 | Leukemia

Figure 5

From: The DNA methyltransferase inhibitors azacitidine, decitabine and zebularine exert differential effects on cancer gene expression in acute myeloid leukemia cells

Figure 5

(a) Verification of drug-specific effects on expression and DNA methylation of the CDKN1C, HOXA9 and HGF genes. The top panel illustrates the expression level of each gene in Kasumi-1 cells as measured by U133A oligonucleotide array. The middle panel depicts relative expression levels as determined by quantitative real-time PCR, using RNA from untreated cells as calibrator to generate standard curves. ACTB was used as endogenous control gene to standardize RNA input. The bottom panel shows changes in DNA methylation at the CDKN1C promoter (HOXA9 or HGF, not done). Error bars represent one standard deviation. (b) Expression and DNA methylation of the ID1, ID2, ID3, RAB13, DLK1 and LIMS1 genes in untreated Kasumi-1 cells and after treatment with AraC, 5AC, DAC or Zeb, as measured by U133A oligonucleotide array (probe sets 208937_s_at, 213931_at, 207826_s_at, 202252_at, 209560_s_at and 212687_at). The LIMS1 gene, which has no cytosine-phosphate-guanine (CpG) island, was not tested for methylation. Error bars represent one standard deviation.

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