Effects of AraC, 5AC, DAC and Zeb on cell cycle, rate of apoptosis and cell cycle of Kasumi-1 cells. (a) Cell-cycle distribution as measured by cellular DNA content based on propidium iodide incorporation. Error bars indicate one standard deviation (three replicate experiments). (b) Induction of apoptosis. Flow cytometry analysis of Annexin V positivity (early apoptosis) and propidium iodide incorporation (late apoptosis) after treatment is shown. (c, d) Induction of differentiation. The shaded histogram shows the level of granulocytic (c) and monocytic (d) antigen expression as measured by CD11b or CD14 positivity in flow cytometry. The black curve represents healthy peripheral blood leukocytes used as positive control.