Erratum: Regulation of IL-2 expression by transcription factor BACH2 in umbilical cord blood CD4+T cells

Correction to: Leukemia (2008) 22, –; 4 September 2008; doi:10.1038/leu.2008.234

Since the publication of the above article, the authors have identified errors in Figures 1 and 2.

Figure 1
figure1

(a) Relative Broad-complex-Tramtrack-Bric-a-Brac and Cap‘n’collar homology 1 bZip transcription factor 2 (BACH2) and nuclear factor of activated T cells (NFAT1) mRNA expression in umbilical cord blood (UCB) and adult peripheral blood (PB) CD4+ T cells. qRT-PCR of resting and CD3/CD28-stimulated UCB and adult CD4+ T cells was performed. Data are presented as the relative mRNA expression in three different UCB CD4+ T-cell preparations and at least three different adult PB CD4+ T-cell preparations. BACH2 mRNA expression was up to fourfold higher in UCB CD4+ compared with adult controls. Error bars represent s.e.m. (**P<0.05). (b) BACH2 protein expression in adult PB and UCB CD4+ T cells. Total resting UCB and adult PB CD4+ T cells were analyzed by western blot for BACH2. BACH2 protein in total UCB CD4+ T cells, compared with total adult PB CD4+ T cells, was 3.9 (±0.072)-fold higher (P=0.0001). BACH2 was normalized against the control β-actin. (c) Protein expression of BACH2 in naive adult PB and UCB CD4+CD45RA+ T cells. Resting naive UCB and adult CD4+ T cells were analyzed by western blot for BACH2. BACH2 protein in UCB naive CD4+ T cells compared with adult naive CD4+ T cells was 1.63 (±0.545)-fold higher (P=0.044). BACH2 was normalized against the control β-actin. Images are representative of multiple western blots from at least three different UCB units and adult samples.

Figure 2
figure2

Broad-complex-Tramtrack-Bric-a-Brac and Cap‘n’collar homology 1 bZip transcription factor 2 (BACH2) and interleukin (IL)-2 effectively inhibited by BACH2 siRNA in UCB CD4+ T cells. Umbilical cord blood (UCB) CD4+ T cells were transfected with BACH2 siRNA or control siRNA. At 24 h after transfection, an aliquot of cells was harvested (resting), and the remaining cells were stimulated with anti-CD3/CD28 antibodies for 6 h. (a) qRT-PCR analysis of nuclear factor of activated T cells (NFAT1)- and NFAT1-dependent genes in UCB CD4+ T cells transfected with BACH2 siRNA. Relative mRNA expression of BACH2, NFAT1, and IL-2 was compared between BACH2 siRNA-treated and control siRNA-treated UCB CD4+ T cells. Results are the average of three separate knockdown transfections with three different UCB units. Error bars represent s.e.m. (**P<0.002). (b) BACH2 expression in UCB CD4+ T cells transfected with BACH2 siRNA. CD4+ T cells from cord units (n>3) were treated with BACH2 siRNA (+) or control siRNA (−). Whole cell lysates from BACH2 siRNA-treated and control siRNA-treated UCB CD4+ T cells were analyzed for BACH2 by western blot. β-Actin was used as a loading control for all blots. BACH2 protein expression was undetectable in BACH2 siRNA treated UCB CD4+ T cells. (c) IL-2 expression in UCB CD4+ T cells transfected with BACH2 siRNA. Whole cell lysates from BACH2 siRNA-treated and control siRNA-treated UCB CD4+ T cells were analyzed for IL-2 by western blot. IL-2 protein was not detectable in BACH2 siRNA-treated UCB CD4+ T cells. (d) BACH2 and IL-2 protein expression in BACH2 siRNA-treated UCB CD4+ T cells. Results are the average of different cord blood units (n>3) treated with BACH2 siRNA or with control siRNA analyzed from at least three different western blots. The image intensity was normalized against β-Actin. Error bars represent the s.e.m. BACH2 protein decreased by 51.7±7.2% (P=0.001) of control UCB CD4+ T cells, whereas the IL-2 protein decreased by 78.9±12% (P=0.0004) of control UCB CD4+ T cells.

The correct figures are shown here.

The authors apologize for any inconvenience caused.

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The online version of the original article can be found at 10.1038/leu.2008.234

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Lesniewski, M., Haviernik, P., Weitzel, R. et al. Erratum: Regulation of IL-2 expression by transcription factor BACH2 in umbilical cord blood CD4+T cells. Leukemia 22, 2293–2294 (2008). https://doi.org/10.1038/leu.2008.292

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