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Alterations of NFIA in chronic malignant myeloid diseases

Leukemia volume 23, pages 583–585 (2009)Cite this article

To determine whether NFIA could have a function in early leukemogenesis, we searched for alterations of the NFIA gene in 89 chronic hematopoietic diseases by using array-comparative genomic hybridization (aCGH) and DNA sequencing. Our series comprised 35 chronic myelomonocytic leukemias and 54 myeloproliferative disorders including 8 cases of polycythemia vera (PV), 42 cases of essential thrombocythemia and 4 cases of idiopathic myelofibrosis. The aCGH experiments used high-density oligonucleotide microarrays (Hu-244A, Agilent Technologies, Massy, France) as previously described.10 aCGH was done on 84 samples. We sequenced exons 2–11 of the gene for 48 samples (all chronic myelomonocytic leukemia, 5 essential thrombocythemia and 8 PV), and only exon 2 for the remaining 41 myeloproliferative disorder samples (primers are available on demand).

We found alterations of the NFIA gene in two patients, mentioned here as patients 1 and 2.

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Acknowledgements

This work was supported by Inserm, Institut Paoli-Calmettes and a grant from Fondation de France to VGB (Comité Leucémie, 2007).

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Author notes

  1. F Bernard and V Gelsi-Boyer: These authors contributed equally to this work.

Authors and Affiliations

  1. Centre de Recherche en Cancérologie de Marseille, Laboratoire d'Oncologie Moléculaire, UMR891 Inserm, Institut Paoli-Calmettes, Marseille, France

    F Bernard, V Gelsi-Boyer, A Murati, V Trouplin, J Adélaïde, S Olschwang, M Chaffanet, M J Mozziconacci & D Birnbaum

  2. Département de BioPathologie, Institut Paoli-Calmettes, Marseille, France

    V Gelsi-Boyer, A Murati, S Olschwang & M J Mozziconacci

  3. Université de la Méditerranée, Marseille, France

    V Gelsi-Boyer & N Vey

  4. U790 Inserm, Université Paris XI, Institut Gustave Roussy, Villejuif, France

    S Giraudier & W Vainchenker

  5. Département d'Hématologie, Institut Paoli-Calmettes, Marseille, France

    J Rey & N Vey

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Correspondence to D Birnbaum.

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Bernard, F., Gelsi-Boyer, V., Murati, A. et al. Alterations of NFIA in chronic malignant myeloid diseases. Leukemia 23, 583–585 (2009). https://doi.org/10.1038/leu.2008.228

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