CDX2 binds to sequences upstream of the ST6GalNAc-I start codon in human intestinal metaplasia. ChIP was performed in vivo in a fragment containing IM foci (H&E image at 40x magnification) and in intestinal mucosa and normal stomach as controls for CDX2 binding. Purified DNA was analysed by qPCR using specific primers covering six regions of the ST6GalNAc-I promoter and another set of primers encompassing the Cdx2-binding site on the sucrase-isomaltase (SI) promoter as a positive control, except for normal gastric mucosa, where SI is absent. Fold enrichments are expressed as ratios of the IP:CDX2 signal to that of the IP:IgG signal and calculated by extrapolation from a standard curve of input DNA dilutions. The results are expressed as mean±s.d. of the triplicates from a representative experiment (*P<0.05 and **P<0.01).