CDX2 binds to sequences upstream of the human ST6GalNAc-I gene. (a) Analysis of a 1774-bp fragment upstream the ST6GalNAc-I start codon (+201). Putative CDX-binding regions (BR) are identified with numbers. Each site was arbitrarily identified as BR1–BR9. (b) CDX2 binding to ST6GalNAc-I promoter in Caco-2 cells at Day +6 analysed by chromatin immunoprecipitation. Purified DNA was analysed by qPCR using specific primers covering six regions of the ST6GalNAc-I promoter. Fold enrichments are expressed as ratios of the IP:CDX2 signal to that of the IP:IgG signal and calculated by extrapolation from a standard curve of input DNA dilutions. The results are expressed as mean±s.d. of the triplicates from a representative experiment (*P<0.05 and **P<0.01).