Angiogenesis in mice skin after wounding. (a) Days 7 and 11 Acta2+/+, Acta2+/−, and Acta2−/− mice wound sections were evaluated by staining with anti-CD31 antibody. Representative micrographs are shown and CD31-positive vessels were indicated by black arrows. Scale bars, 100 μm for × 40 images. (b) Levels of vascularization were determined by the number of vessels stained positive for CD31 per high-power field in wound area. Data are represented as means±s.e.m. (n=3 per genotype). *P<0.05 tested by one-way ANOVA with Bonferroni’s post hoc test, performed on Acta2+/+ vs Acta2−/− for day 7 mice tissue, on Acta2+/+ vs Acta2+/−, Acta2+/− vs Acta2−/−, and Acta2+/+ vs Acta2−/− for day 11 mice tissue, respectively. ANOVA, analysis of variance. A full color version of this figure is available at the Laboratory Investigation journal online.