ACTA2, ACTB, and ACTG1 expression in the remodeling phase of repair. (a) Representative human unwounded skin. (b) Scar specimens investigated by IHC staining are shown to reveal the expression pattern of ACTA2, ACTB, and ACTG1. Scale bars, 400 μm for × 4 (upper panel of (a) and (b)) and 100 μm for × 40 (lower panel of (a) and (b)) images; e, epidermis; d, dermis. (c) The staining intensity of human unwounded skin tissue and contraction scar over time was quantified. Data are expressed as mean±s.e.m. *P<0.05, tested by one-way ANOVA with Bonferroni’s post hoc test, performed on 6- to 10-month vs >10-month scar for ACTB expression. ANOVA, analysis of variance; IHC, immunohistochemistry.