Genetic loss of osteopontin (OPN) and tumor necrosis factor-α receptor-1 (TNFR1) has no effect on the composition and density of the inflammatory infiltrate in response to DDC feeding. Immunohistochemistry for F4/80 (a–d) and CD11b (e–h) in chow-fed wild type (WT/chow), WT 4 weeks DDC-fed (WT/DDC), OPN knock-out 4 weeks DDC-fed (OPN/DDC) and TNFR1 knock-out 4 weeks DDC-fed (TNFR/DDC) mice. (b–d) Please note that in spite of the OPN or TNFR1 loss 4 weeks DDC-fed knock-out mice show a pronounced hepatic inflammation with no differences in regard to density and lobular distribution of the immunoreactivity. (e–h) In contrast to the observed F4/80 staining pattern the CD11b signal is concentrated and accentuated to portal fields and bile ducts again showing no reduction of the inflammatory response in 4 weeks DDC-fed knock-out mice indicating a comparable inflammatory response compared with WT controls. pv, portal vein; bd, bile duct. Original magnification × 20.