Figure 1 | Laboratory Investigation

Figure 1

From: The role of osteopontin and tumor necrosis factor alpha receptor-1 in xenobiotic-induced cholangitis and biliary fibrosis in mice

Figure 1

Genetic loss of osteopontin (OPN) and tumor necrosis factor-α receptor-1 (TNFR1) has no effect on the composition and density of the inflammatory infiltrate in response to DDC feeding. Immunohistochemistry for F4/80 (ad) and CD11b (eh) in chow-fed wild type (WT/chow), WT 4 weeks DDC-fed (WT/DDC), OPN knock-out 4 weeks DDC-fed (OPN/DDC) and TNFR1 knock-out 4 weeks DDC-fed (TNFR/DDC) mice. (bd) Please note that in spite of the OPN or TNFR1 loss 4 weeks DDC-fed knock-out mice show a pronounced hepatic inflammation with no differences in regard to density and lobular distribution of the immunoreactivity. (eh) In contrast to the observed F4/80 staining pattern the CD11b signal is concentrated and accentuated to portal fields and bile ducts again showing no reduction of the inflammatory response in 4 weeks DDC-fed knock-out mice indicating a comparable inflammatory response compared with WT controls. pv, portal vein; bd, bile duct. Original magnification × 20.

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