Abstract
The authors evaluated the efficiency and safety of a technique for drawing blood from the cranial vena cava of the hamster (Mesocricetus auratus). They collected blood from Golden Syrian hamsters (n = 15) and from epileptic hamsters (GASH/Sal strain; n = 10) that were anesthetized with isoflurane. Four epileptic hamsters constituted a control group that underwent anesthesia but not blood collection. For blood sampling, a needle connected to a syringe was inserted at a site between 0.2 and 0.6 cm from the manubrium; the depth of insertion was 0.3–1.0 cm. Blood collection was successful in all hamsters except one, and the volume of collected blood ranged from 0.2 ml to 1.5 ml. All hamsters recovered quickly from the procedure, and none showed obvious signs of pain or stress. At necropsy (24 h after the procedure), six subjects showed small local hematomas, and eight showed vascular lacerations of limited severity. Lungs and hearts did not show any damage related to vein puncture. This relatively simple blood collection technique seems to be efficient and safe in hamsters.
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References
Hem, A., Smith, A.J. & Solberg, P. Saphenous vein puncture for blood sampling of the mouse, rat, hamster, gerbil, guinea pig, ferret and mink. Lab. Anim. 32, 364–368 (1998).
Winsett, O.E., Townsend, C.M. & Thompson, J.C. Rapid and repeated blood sampling in the conscious laboratory rat: a new technique. Am. J. Physiol. 245, 145–146 (1985).
Phillips, W.A., Stafford, W.W. & Stuut, J. Jugular vein technique for serial blood sampling and intravenous injection in the rat. Proc. Soc. Exp. Biol. Med. 143, 733–735 (1973).
Sloop, C. & Krause, B. Portal and aortic blood sampling technique in unrestrained rats. Physiol. Behav. 26, 529–533 (1981).
Fluttert, M., Dalm, S. & Oitzl, M.S. A refined method for sequential blood sampling by tail incision in rats. Lab. Anim. 34, 372–378 (2000).
Zeller, W., Weber, H., Panoussis, B., Bürge, T. & Bergmann, R. Refinement of blood sampling from the sublingual vein of rats. Lab. Anim. 32, 369–376 (1998).
Morton, D.B. et al. Removal of blood from laboratory mammals and birds. First report of the BVA/FRAME/RSPCA/UFAW Joint Working Group on Refinement. Lab. Anim. 27, 1–22 (1993).
Walker, W.F. & Homberger, D.G. Anatomy and Dissection of the Rat 3rd edn. (W.H. Freeman & Company, New York, 1997).
Popesko, P., Rajtové, V. & Horák, J. Colour Atlas of Anatomy of Small Laboratory Animals: Volume 2 2nd edn. (Saunders, London, 2002).
Smith, C.A. & Ficken, M.D. Non-surgical cannulation of the vena cava for chronic blood collection in mature swine. Lab. Anim. Sci. 141, 274–278 (1991).
Abrams-Ogg, A.C. et al. The use of an implantable central venous (Hickman) catheter for long-term venous access in dogs undergoing bone marrow transplantation. Can. J. Vet. Res. 56, 382–386 (1992).
Brown, C. Blood collection from the cranial vena cava of the ferret. Lab Anim. (NY) 35, 23–24 (2006).
Jekl, V., Hauptman, K., Jeklová, E. & Knotek, Z. Blood sampling from the cranial vena cava in the Norway rat (Rattus norvegicus). Lab. Anim. 39, 236–239 (2004).
Salvador-Cabos, N. in Ciencia y Tecnología en Protección y Experimentación Animal 1st edn. (eds. Zúñiga, J., Tur Marí, J.A., Milocco, S.N. & Piñeiro, R.) 26–36 (McGraw-Hill, Madrid, 2001).
Van Herck, H. et al. Blood sampling from the retro-orbital plexus, the saphenous vein and the tail vein in rats: comparative effects on selected behavioural and blood variables. Lab. Anim. 35, 131–139 (2001).
Fitzner Toft, M., Petersen, M.H., Dragsted, N. & Hansen, A.K. The impact of different blood sampling methods on laboratory rats under different types of anaesthesia. Lab. Anim. 40, 261–274 (2005).
Altholtz, L.Y., Fowler, K.A., Badura, L.L. & Kovacs, M.S. Comparison of the stress response in rats to repeated isoflurane or CO2:O2 anaesthesia used for restraint during serial blood collection via the jugular vein. J. Am. Assoc. Lab. Anim. Sci. 45, 17–22 (2006).
Muñoz de la Pascua, L. & López, D.E. Establecimiento y Caracterización de una Línea de Hamsters Sirios Propensos a Padecer Convulsiones Audiógenas. Thesis, Univ. Salamanca (2004).
Bancroft, J.D. & Gamble, M. Theory and Practice of Histological Techniques (Churchill Livingstone, New York, 2002).
Schoemaker, N.J. in BSAVA Manual of Exotics Pets 4th edn. (eds. Meredith, A. & Redrobe, S.) 3–101 (British Small Animal Veterinary Association, Quedgeley, UK, 2002).
Goodman, G. in BSAVA Manual of Exotic Pets 4th edn. (eds. Meredith, A. & Redrobe, S.) 26–33 (British Small Animal Veterinary Association, Quedgeley, UK, 2002).
Acknowledgements
We thank Dr. Dolores E. López, Dr. Luis Muñoz de la Pascua and Dr. Carlos de Cabo for providing animals; Dr. Syong Hyong Nam Cha for her invaluable help with the microscopic analyses; and Mrs. María Jesús Castaño-Aroca for her support in the preparation of the manuscript.
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Picazo, M., Benito, P. & García-Olmo, D. Efficiency and safety of a technique for drawing blood from the hamster cranial vena cava. Lab Anim 38, 211–216 (2009). https://doi.org/10.1038/laban0609-211
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DOI: https://doi.org/10.1038/laban0609-211