Abstract
It is difficult to collect untainted urine specimens over short intervals of time during renal studies with rabbits. This is because both the ureters and the bladder of this species are relatively friable and minor manipulation can easily cause intraluminal bleeding. We have developed and refined an effective technique and protocol for placing an indwelling urinary bladder catheter into an anesthetized rabbit. The procedure is easy to perform and completely effective and reliable, allowing high-quality urinary specimens to be collected at intervals of 15–20 min over a period of 3–4 hours during a study of acute metabolic acidosis.
This is a preview of subscription content, access via your institution
Relevant articles
Open Access articles citing this article.
-
The kinetics of inorganic phosphate excretion in the acidotic rabbit during intravenous phosphate loading: a pseudo-ruminant model
Scientific Reports Open Access 04 March 2020
Access options
Subscribe to this journal
We are sorry, but there is no personal subscription option available for your country.
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Kekomaki, M., Rikalainen, H., Ruotsalainen, P. & Bertenyi, C. Correlates of diuretic renography in experimental hydronephrosis. J. Urol. 141, 391–394 (1989).
Andrews, P.M., Khirabadi, B.S. & Bengs, B.C. Using tandem scanning confocal microscopy to predict the status of donor kidneys. Nephron. 91, 148–155 (2002).
Sammut, I.A. et al. Time-dependent impairment of mitochondrial function after storage and transplantation of rabbit kidneys. Transplantation. 69, 1265–1275 (2000).
Hanley, M.J. Studies on acute disease models. Kidney Int. 22, 536–545 (1982).
Dimitrov, R., Kostov, D., Stamatova, K. & Yordanova, V. Anatomotopographical and morphological analysis of normal kidneys of rabbits (Oryctolagus cuniculus). Trakia J. Sci. 10, 79–84 (2012).
Kaplan, H.M. & Timmons, E.H. The Rabbit: a Model for the Principles of Mammalian Physiology and Surgery (Academic Press, Burlington, MA, 1979).
Kurien, B.T., Everds, N.E. & Scofield, R.H. Experimental animal urine collection: a review. Lab. Anim. 38, 333–361 (2004).
Harris, R.H. Jr. & Best, C.F. Ureteral catheter system for renal function studies in conscious rabbits. Lab. Anim. Sci. 29, 781–784 (1979).
Tarloff, J.B. Clearance methods in renal toxicology. in Methods in Renal Toxicology (eds. Zalups, R.K. & Lash, L.H.) 1–17 (CRC Press, Boca Raton, FL, 1996).
Yu, H.L. et al. Stimulation of ammonia production and excretion in the rabbit by inorganic phosphate. Study of control mechanisms. J. Clin. Invest. 58, 557–564 (1976).
Lim, J.S. et al. The evaluation of a dissolvable ureteral catheter in a rabbit model. Korean J. Urol. 47, 895–902 (2006).
Amin, M., Clark, R., Howerton, L.W. & Lich, R. Jr. Terminal loop cutaneous ureterostomy: an experimental study and its clinical application. J. Urol. 118, 383–385 (1977).
Chen, D.S., Donald, D.E. & Romero, J.C. Collection of urine from each kidney in the rabbit without ureteral cannulation. Am. J. Physiol. 233, F615–F617 (1977).
Harkness, J.E. & Wagner, J.E. The Biology and Medicine of Rabbits and Rodents 4th edn. (Williams & Wilkins, Baltimore, MD, 1995).
Hau, J. & Van Hoosier, G.L. Jr. Handbook of Laboratory Animal Science Volume I: Essential Principles and Practices 2nd edn. (CRC Press, Boca Raton, FL, 2003).
Hillyer, E.V. & Queensberry, K. Ferrets, Rabbits, and Rodents: Clinical Medicine and Surgery 1st edn. (W.B. Saunders, Philadelphia, 1997).
Manning, P.J., Ringler, D.H. & Newcomer, C.E. (eds.) The Biology of the Laboratory Rabbit (Academic Press, New York, 1994).
Uthamanthil, R.K. et al. Urinary catheterization of male rabbits: a new technique and a review of urogenital anatomy. JAALAS 52, 180–185 (2013).
Acknowledgements
We thank Dr. Bindhu Nair for helping with the compiling of the references.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing financial interests.
Rights and permissions
About this article
Cite this article
Walsh, P., O'Donovan, D. Collection of untainted urinary specimens from the bladder of an anesthetized rabbit. Lab Anim 45, 112–114 (2016). https://doi.org/10.1038/laban.953
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/laban.953