Mammary tumors similar to those observed in women can be induced in rats by intraperitoneal administration of N-methyl-N-nitrosourea. Determining tumor volume is a useful and quantitative way to monitor tumor progression. In this study, the authors measured dimensions of rat mammary tumors using a caliper and using real-time compound B-mode ultrasonography. They then used different formulas to calculate tumor volume from these tumor measurements and compared the calculated tumor volumes with the real tumor volume to identify the formulas that gave the most accurate volume calculations. They found that caliper and ultrasonography measurements were significantly correlated but that tumor volumes calculated using different formulas varied substantially. Mammary tumors seemed to take on an oblate spheroid geometry. The most accurate volume calculations were obtained using the formula V = (W2 × L)/2 for caliper measurements and the formula V = (4/3) × π × (L/2) × (L/2) × (D/2) for ultrasonography measurements, where V is tumor volume, W is tumor width, L is tumor length and D is tumor depth.
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Parkin, D.M., Bray, F., Ferlay, J. & Pisani, P. Global cancer statistics, 2002. CA Cancer J. Clin. 55, 74–108 (2005).
Verma, R. et al. Comparison of clinical assessment, mammography and ultrasound in pre-operative estimation of primary breast-cancer size: a practical approach. Internet J. Surg. 16 (2008).
Clarke, R. Animal models of breast cancer: their diversity and role in biomedical research. Breast Cancer Res. Treat. 39, 1–6 (1996).
McCormick, D.L., Adamowski, C.B., Fiks, A. & Moon, R.C. Lifetime dose-response relationships for mammary tumor induction by a single administration of N-methyl-N-nitrosourea. Cancer Res. 41, 1690–1694 (1981).
Jensen, M.M., Jørgensen, J.T., Binderup, T. & Kjær, A. Tumor volume in subcutaneous mouse xenografts measured by microCT is more accurate and reproducible than determined by 18F-FDG-microPET or external caliper. BMC Med. Imaging 16, 8–16 (2008).
Hoffman-Goetz, L. et al. Possible mechanisms mediating an association between physical activity and breast cancer. Cancer 83, 621–628 (1998).
Westerlind, K.C. Physical activity cancer prevention–mechanisms. Med. Sci. Sport. Exer. 35, 1834–1840 (2003).
Eichelerger, L.E. et al. Predicting tumor volume in radical prostatectomy specimens from patients with prostate cancer. Am. J. Clin. Pathol. 120, 386–391 (2003).
Davis, P.L. et al. Breast cancer measurements with magnetic resonance imaging, ultrasonography, and mammography. Breast Cancer Res. Treat. 37, 1–9 (1996).
Pritt, B., Ashikaga, T., Oppenheimer, R.G. & Weaver, D.L. Influence of breast cancer histology on the relationship between ultrasound and pathology tumor size measurements. Modern Pathol. 17, 905–910 (2004).
Berg, W.A. et al. Diagnostic accuracy of mammography, clinical examination, US, and MR imaging in preoperative assessment of breast cancer. Radiology 233, 830–849 (2004).
Fornage, B.D., Toubas, O. & Deshayes, J.L. Role of real-time sonography in assessment of breast diseases: a review of 200 cases (Abstr). Radiology 157, 54 (1985).
Gullino, P.M., Pettigrew, H.M. & Grantham, F.H. N-nitrosomethylurea as mammary gland carcinogen in rats. J. Natl. Cancer Inst. 54, 401–414 (1975).
Komárek, V. Gross anatomy. in The Laboratory Mouse (eds. Hedrich, H.J. & Bullock, G.) 253–257 (Elsevier Academic, Boston, 2004).
Kubatka, P. et al. Effects of tamoxifen and melatonin on mammary gland cancer induced by N-methyl-N-nitrosourea and by 7,12-dimethylbenz(a)anthracene, respectively, in female Sprague-Dawley rats. Folia Biol. 47, 5–10 (2001).
Bousquet, P.F. et al. Preclinical evaluation of LU 79553: a novel bis-naphthalimide with potent antitumor activity. Cancer Res. 55, 1176–1180 (1995).
Harris, R.E., Alshafie, G.A., Abou-Issa, H. & Seibert, K. Chemopreventive of breast cancer in rats by celecoxib, a ciclooxygenase 2 inhibitor. Cancer Res. 60, 2101–2103 (2000).
Forbes, D., Blom, H., Kostomitsopoulos, N., Moore, G. & Perretta, G. Euroguide: On the Accommodation and Care of Animals Used for Experimental and Other Scientific Purposes (Federation of European Laboratory Animal Science Associations, London, 2007).
Clarys, J.P. & Marfell-Jones, M.J. Soft tissue segmentation of the body and fractionation of the upper and lower limbs. Ergonomics 37, 217–229 (1994).
McPhail, L.D. & Robinson, S.P. Intrinsic susceptibility MR imaging of chemically induced rat mammary tumors: relationship to histologic assessment of hypoxia and fibrosis. Radiology 254, 110–118 (2010).
Kobayashy, T. et al. Value of prostate volume measurement using transabdominal ultrasonography for the improvement of prostate-specific antigen-based cancer detection. Int. J. Urol. 12, 881–885 (2005).
Yang, C.H., Wang, S.J., Lin, A.T.L., Jen, Y.M. & Lin, C.A. Evaluation of prostate volume by transabdominal ultrasonography with modified ellipsoid formula at different stages of benign prostatic hyperplasia. Ultrasound Med. Biol. 37, 331–337 (2011).
Carlsson, G., Gullberg, B. & Hafström, L. Estimation of liver tumor volume using different formulas—an experimental study in rats. J. Cancer Res. Clin. Oncol. 105, 20–23 (1983).
Russo, J. & Russo, I.H. Experimentally induced mammary tumors in rats. Breast Cancer Res. Treat. 39, 7–20 (1996).
Perše, M., Cerar, A. & Štrukelj, B. N-methylnitrosourea induced breast cancer in rat, the histopathology of the resulting tumours and its drawbacks as a model. Pathol. Oncol. Res. 15, 115–121 (2009).
Weber, W.A. & Wieder, H. Monitoring chemotherapy and radiotherapy of solid tumors. Eur. J. Nucl. Med. Mol. Imaging 33, 27–37 (2006).
Girit, I.C., Jure-Kunkel, M. & McIntyre, K.W. A structured light-based system for scanning subcutaneous tumors in laboratory animals. Comp. Med. 58, 264–270 (2008).
Fornage, B.D., Toubas, O. & Morel, M. Clinical, mammographic, and sonographic determination of preoperative breast cancer size. Cancer 60, 765–771 (1987).
Lee, J., Koh, D. & Ong, C.N. Statistical evaluations of agreement between two methods for measuring a quantitative variable. Comput. Biol. Med. 19, 61–70 (1989).
Denis, F., Paon, L. & Tranquart, F. Radiosensitivity of rat mammary tumors correlates with early vessel changes assessed by Power Doppler sonography. J. Ultrasound Med. 22, 921–929 (2003).
Pollok, K.E. et al. In vivo measurements of tumor metabolism and growth after administration of enzastaurin using small animal FDG positron emission tomography. J. Oncol. 2009, 1–8 (2009).
This work was supported by the Portuguese Foundation for Science and Technology (PTDC/DES/114122/2009 and Pest-OE/AGR/UI0772/2011 unity) and by the European Regional Development Fund (COMPETE, FCOMP-01-0124-FEDER-014707/022692).
The authors declare no competing financial interests.
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Faustino-Rocha, A., Oliveira, P., Pinho-Oliveira, J. et al. Estimation of rat mammary tumor volume using caliper and ultrasonography measurements. Lab Anim 42, 217–224 (2013). https://doi.org/10.1038/laban.254
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