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Mechanical ventilation of mice under general anesthesia during experimental procedures

Lab Animal volume 42, pages 253–257 (2013)Cite this article

Abstract

Mechanical ventilation is frequently used in patients under general anesthesia during invasive procedures. Invasive animal experiments similarly require the maintenance of normal hemodynamic and pulmonary parameters during long-term general anesthesia. The authors describe a method for mechanical ventilation of mice. Mice were ventilated and monitored for up to 8 h of general anesthesia during surgery. Hemodynamic and pulmonary parameters remained within the normal ranges. The authors believe that this ventilation technique can be of great value for experimental procedures in mice that require general anesthesia.

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Figure 1: Intubation of a mouse.
Figure 2: Diagram of cannula placement in the carotid artery.
Figure 3: Hemodynamic parameters during 8 h of mechanical ventilation (n = 9).

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Authors and Affiliations

  1. Department of Anesthesiology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands

    Ilona van den Brink, Francien van de Pol,  Michiel Vaneker,  Matthijs Kox,  Willem-Jan Schellekens &  Gert Jan Scheffer

  2. Central Animal Laboratory, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands

    Merel Ritskes-Hoitinga

Authors
  1. Ilona van den Brink
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  2. Francien van de Pol
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  3. Michiel Vaneker
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  4. Matthijs Kox
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  5. Willem-Jan Schellekens
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  6. Merel Ritskes-Hoitinga
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  7. Gert Jan Scheffer
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Correspondence to Ilona van den Brink.

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The authors declare no competing financial interests.

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van den Brink, I., van de Pol, F., Vaneker, M. et al. Mechanical ventilation of mice under general anesthesia during experimental procedures. Lab Anim 42, 253–257 (2013). https://doi.org/10.1038/laban.252

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