Leach, M.C. et al. Aversion to gaseous euthanasia agents in rats and mice. Comp. Med. 52, 249–257 (2002).
McIntyre, A.R. et al. Automated mouse euthanasia in an individually ventilated caging system: system development and assessment. J. Am. Assoc. Lab. Anim. Sci. 46, 65–73 (2007).
Taylor, D.K. & Mook, D.M. Isoflurane waste anesthetic gas concentrations associated with the open-drop method. J. Am. Assoc. Lab. Anim. Sci. 48, 61–64 (2009).
Burkholder, T.H. et al. Comparison of carbon dioxide and argon euthanasia: effects on behavior, heart rate, and respiratory lesions in rats. J. Am. Assoc. Lab. Anim. Sci. 49, 448–453 (2010).
Pritchett, K. et al. Euthanasia of neonatal mice with carbon dioxide. Comp. Med. 55, 275–281 (2005).
Hazzard, K.C., Watkins-Chow, D.E. & Garrett, L.J. Method of euthanasia influences the oocyte fertilization rate with fresh mouse sperm. J. Am. Assoc. Lab. Anim. Sci. 53, 641–646 (2014).
Raj, A.B., Leach, M.C. & Morton, D.B. Carbon dioxide for euthanasia of laboratory animals. Comp. Med. 54, 470–471 (2004).
National Research Council Committee on Recognition and Alleviation of Distress in Laboratory Animals. Recognition and alleviation of distress in laboratory animals (National Academies Press, Washington, D.C., 2008).
Smith, W. & Harrap, S.B. Behavioural and cardiovascular responses of rats to euthanasia using carbon dioxide gas. Lab. Anim. 31, 337–346 (1997).
Thomas, A.A., Flecknell, P.A. & Golledge, H.D. Combining nitrous oxide with carbon dioxide decreases the time to loss of consciousness during euthanasia in mice–refinement of animal welfare? PLoS ONE 7, e32290 (2012).
Pecaut, M.J. et al. Modification of immunologic and hematologic variables by method of CO2 euthanasia. Comp. Med. 50, 595–602 (2000).
Dunn, J.D., Cometto-Muniz, J.E. & Cain, W.S. Nasal reflexes: reduced sensitivity to CO2 irritation in cigarette smokers. J. Appl. Toxicol. 2, 176–178 (1982).
Anton, F., Euchner, I. & Handwerker, H.O. Psychophysical examination of pain induced by defined CO2 pulses applied to the nasal mucosa. Pain 49, 53–60 (1992).
Danneman, P.J., Stein, S. & Walshaw, S.O. Humane and practical implications of using carbon dioxide mixed with oxygen for anesthesia or euthanasia of rats. Lab. Anim. Sci. 47, 376–385 (1997).
Wise, P.M., Wysocki, C.J. & Radil, T. Time–intensity ratings of nasal irritation from carbon dioxide. 28, 751–760 2003.
Cooper, J., Mason, G. & Raj, M. Determination of the aversion of farmed mink (Mustela vison) to carbon dioxide. Vet. Rec. 143, 359–361 (1998).
Raj, A.B. Aversive reactions of turkeys to argon, carbon dioxide and a mixture of carbon dioxide and argon. Vet. Rec. 138, 592–593 (1996).
Llonch, P. et al. Stunning pigs with nitrogen and carbon dioxide mixtures: effects on animal welfare and meat quality. Animal 6, 668–675 (2012).
Shusterman, D. & Avila, P.C. Real-time monitoring of nasal mucosal pH during carbon dioxide stimulation: implications for stimulus dynamics. Chem. Senses 28, 595–601 (2003).
Hu, J. et al. Detection of near-atmospheric concentrations of CO2 by an olfactory subsystem in the mouse. Science 317, 953–957 (2007).
Coates, E.L. Olfactory CO(2) chemoreceptors. Respir. Physiol. 129, 219–229 (2001).
Dangers, L., Laviolette, L., Similowski, T. & Morélot-Panzini, C. Interactions between dyspnea and the brain processing of nociceptive stimuli: experimental air hunger attenuates laser-evoked brain potentials in humans. Front. Physiol. 6, 358 (2015).
Liotti, M. et al. Brain responses associated with consciousness of breathlessness (air hunger). Proc. Natl. Acad. Sci. 98, 2035–2040 (2001).
Artwohl, J. et al. Report of the ACLAM Task Force on Rodent Euthanasia. J. Am. Assoc. Lab. Anim. Sci. 45, 98–105 (2006).
American Veterinary Medical Association. AVMA Guidelines for the Euthanasia of Animals: 2013 Edition, 2013 (https://www.avma.org/KB/Policies/Documents/euthanasia.pdf).
Moody, C.M., Chua, B. & Weary, D.M. The effect of carbon dioxide flow rate on the euthanasia of laboratory mice. Lab. Anim. 48, 298–304 (2014).
Sadler, L.J. et al. Effects of flow rate and gas mixture on the welfare of weaned and neonate pigs during gas euthanasia. J. Anim. Sci. 92, 793–805 (2014).
Sharp, J., Azar, T. & Lawson, D. Comparison of carbon dioxide, argon, and nitrogen for inducing unconsciousness or euthanasia of rats. J. Am. Assoc. Lab. Anim. Sci. 45, 21–25 (2006).
Wong, D., Makowska, I.J. & Weary, D.M. Rat aversion to isoflurane versus carbon dioxide. Biol. Lett. 9, 20121000 (2012).
Leach, M.C. et al. Degrees of aversion shown by rats and mice to different concentrations of inhalational anaesthetics. Vet. Rec. 150, 808–815 (2002).
Valentine, H., Williams, W.O. & Maurer, K.J. Sedation or inhalant anesthesia before euthanasia with CO2 does not reduce behavioral or physiologic signs of pain and stress in mice. J. Am. Assoc. Lab. Anim. Sci. 51, 50–57 (2012).
Becker, K. et al. Low dose isoflurane exerts opposing effects on neuronal network excitability in neocortex and hippocampus. PLoS ONE 7, e39346 (2012).
Komatsu, H. & Ogli, K. Opisthotonus during exposure to isoflurane, enflurane, and halothane in mice. Anesthesiology 67, 771–774 (1987).
Bowen, M.T. et al. Aggregation in quads but not pairs of rats exposed to cat odor or bright light. Behav. Processes 90, 331–336 (2012).
Huang, Y., Zhou, W. & Zhang, Y. Bright lighting conditions during testing increase thigmotaxis and impair water maze performance in BALB/c mice. Behav. Brain Res. 226, 26–31 (2012).
Krysiak, M.E. et al. The effect of ecologically relevant variations in light level on the performance of Mongolian gerbils on two visual tasks. Behav. Processes 88, 135–141 (2011).
Takao, K. & Miyakawa, T. Light/dark transition test for mice. J. Vis. Exp. 13, 104 (2006).
Miller, S.M., Piasecki, C.C. & Lonstein, J.S. Use of the light-dark box to compare the anxiety-related behavior of virgin and postpartum female rats. Pharmacol. Biochem. Behav. 100, 130–137 (2011).
Institute for Laboratory Animal Research. Guide for the Care and Use of Laboratory Animals 8th edn. (National Academies Press, Washington, DC, 2011).
Semple-Rowland, S.L. & Dawson, W.W. Retinal cyclic light damage threshold for albino rats. Lab. Anim. Sci. 37, 289–298 (1987).
Chakravarti, S. & Basu, S. Modern anaesthesia vapourisers. Indian J. Anaesth. 57, 464–471 (2013).
Bowers, S.L. et al. Stressor-specific alterations in corticosterone and immune responses in mice. Brain Behav. Immun. 22, 105–113 (2008).
De Boer, S.F. et al. Plasma catecholamine, corticosterone and glucose responses to repeated stress in rats: effect of interstressor interval length. Physiol. Behav. 47, 1117–1124 (1990).
Girard-Joyal, O. et al. Age and sex differences in c-Fos expression and serum corticosterone concentration following LPS treatment. Neuroscience 305, 293–301 (2015).
Gong, S. et al. Dynamics and correlation of serum cortisol and corticosterone under different physiological or stressful conditions in mice. PLoS ONE 10, e0117503 (2015).
Vahl, T.P. et al. Comparative analysis of ACTH and corticosterone sampling methods in rats. Am. J. Physiol. Endocrinol. Metab. 289, E823–E828 (2005).
Nyuyki, K.D. et al. Comparison of corticosterone responses to acute stressors: chronic jugular vein versus trunk blood samples in mice. Stress 15, 618–626 (2012).
Zamora-Gonzalez, E.O. et al. A chronic combinatory stress model that activates the HPA axis and avoids habituation in BALB/C mice. J. Neurosci. Methods 213, 70–75 (2013).
Tuli, J.S., Smith, J.A. & Morton, D.B. Corticosterone, adrenal and spleen weight in mice after tail bleeding, and its effect on nearby animals. Lab. Anim. 29, 90–95 (1995).
Denmark, A. et al. The effects of chronic social defeat stress on mouse self-grooming behavior and its patterning. Behav. Brain Res. 208, 553–559 (2010).
Hyde, J.F. & Jerussi, T.P. Sexual dimorphism in rats with respect to locomotor activity and circling behavior. Pharmacol. Biochem. Behav. 18, 725–729 (1983).
Deacon, R.M. Digging and marble burying in mice: simple methods for in vivo identification of biological impacts. Nat. Protoc. 1, 122–124 (2006).
Conlee, K.M. et al. Carbon dioxide for euthanasia: concerns regarding pain and distress, with special reference to mice and rats. Lab. Anim. 39, 137–161 (2005).
Hewett, T.A. et al. A comparison of euthanasia methods in rats, using carbon dioxide in prefilled and fixed flow rate filled chambers. Lab. Anim. Sci. 43, 579–582 (1993).
Rault, J.L. et al. Nitrous oxide as a humane method for piglet euthanasia: Behavior and electroencephalography (EEG). Physiol. Behav. 151, 29–37 (2015).
Al-Mousawi, A.M. et al. Impact of anesthesia, analgesia, and euthanasia technique on the inflammatory cytokine profile in a rodent model of severe burn injury. Shock 34, 261–268 (2010).
Johnson, R.A. et al. Comparison of isoflurane with sevoflurane for anesthesia induction and recovery in adult dogs. Am. J. Vet. Res. 59, 478–481 (1998).
Altholtz, L.Y. et al. Comparison of the stress response in rats to repeated isoflurane or CO2:O2 anesthesia used for restraint during serial blood collection via the jugular vein. J. Am. Assoc. Lab. Anim. Sci. 45, 17–22 (2006).
Blom, H.J. et al. Preferences of mice and rats for types of bedding material. Lab. Anim. 30, 234–244 (1996).