Abstract
Accurate mouse sexing is vital when conducting research examining sexual dimorphisms. Late fetal and newborn mouse pups are more immature than many previously described sexing methods allow. This study compares the sexing accuracy of a newly described internal gonad sexing method to a recently described peritoneal pigmentation sexing method in embryonic day 20 C57BL/6J mouse pups, using Sry genotyping to confirm the sex. The internal gonad sexing method was found to be highly accurate, while the peritoneal pigmentation method was slightly less accurate. Therefore, while Sry genotyping remains the gold standard, immediate and less expensive sexing methods can be performed accurately as early as the late fetal period in C57BL/6J mice.
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References
Greenham, L.W. & Greenham, V. Sexing mouse pups. Lab. Anim. 11, 181–184 (1977).
Wolterink-Donselaar, I.G., Meerding, J.M. & Fernandes, C. A method for gender determination in newborn dark pigmented mice. Lab Anim. (NY) 38, 35–38 (2009).
Whyte, J.J., Roberts, R.M. & Rosenfeld, C.S. Fluorescent in situ hybridization for sex chromosome determination before and after fertilization in mice. Theriogenology 67, 1022–1031 (2007).
Cook, M. The Anatomy of the Laboratory Mouse (Academic Press Inc., New York, 1965).
Chen, H. et al. Identification of novel markers of mouse fetal ovary development. PLoS ONE 7, e41683 (2012).
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Deeney, S., Powers, K. & Crombleholme, T. A comparison of sexing methods in fetal mice. Lab Anim 45, 380–384 (2016). https://doi.org/10.1038/laban.1105
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DOI: https://doi.org/10.1038/laban.1105
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