Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Toxoplasmosis in pregnancy: determination of IgM, IgG and avidity in filter paper-embedded blood

Abstract

Objective:

To detect immunoglobulin M (IgM) anti-Toxoplasma gondii antibodies and determine immunoglobulin G (IgG) titer and avidity in filter paper-embedded blood (FPEB) samples of pregnant women.

Study Design:

A total of 100 FPEB samples of pregnant women (30 positive and 70 negative) were analyzed for anti-T. gondii-specific IgM antibodies. Eleven and nine pairs of serum and FPEB samples were used to standardize IgG titration and avidity, respectively. Then, the correlation of avidity results was determined with 23 serum/FPEB pairs from IgG-positive cases.

Result:

IgM detection in FPEB was 92% sensitive and 100% specific. The titration of IgG antibodies in FPEB correlated with that of serum (r 0.9). Significant difference in avidity between the acute and the undetermined/chronic cases was observed in both samples. As expected, no correlation was found between IgM levels and avidity.

Conclusion:

The FPEB is useful to infer infection phase, and thus to speed clinical decisions in congenital toxoplasmosis management.

This is a preview of subscription content

Access options

Buy article

Get time limited or full article access on ReadCube.

$32.00

All prices are NET prices.

Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7

References

  1. Ambroise TP, Petersen E . Congenital toxoplasmosis. Scientific Background, Clinical Management and Control. Springer-Verlag: France, 2000, 324 pp.

    Google Scholar 

  2. Desmonts G, Naot Y, Remington JS . Immunoglobin M-immunosorbent agglutination assay for diagnosis of infectious diseases: diagnosis of acute congenital and acquired Toxoplasma infections. J Clin Microbiol 1981; 14: 486–491.

    CAS  PubMed  PubMed Central  Google Scholar 

  3. Jenum PA, Stray-Pedersen B . Development of specific immunoglobulins G, M, and A following primary Toxoplasma gondii infection in pregnant women. J Clin Microbiol 1998; 36: 2907–2913.

    CAS  PubMed  PubMed Central  Google Scholar 

  4. Bessieres MH, Berrebi A, Rolland M, Bloom MC, Roques C, Cassaing S et al. Neonatal screening for congenital toxoplasmosis in a cohort of 165 women infected during pregnancy and influence of in utero treatment on the results of neonatal test. Eur J Obst Gyn Rep Biol 2001; 94: 37–45.

    CAS  Article  Google Scholar 

  5. Gras L, Gilbert RE, Wallon M, Peyron F, Cortina-Borja M . Duration of the IgM response in women acquiring Toxoplasma gondii during pregnancy: implications for clinical practice and cross-sectional incidence studies. Epidemiol Infect 2004; 132: 541–548.

    CAS  Article  Google Scholar 

  6. Araujo PR, Ferreira AW . Avidity of IgG antibodies against excreted/secreted antigens of Toxoplasma gondii: immunological marker for acute recent toxoplasmosis. Rev Soc Bras Med Trop 2008; 41: 142–147.

    Article  Google Scholar 

  7. Prince HE, Wilson M . Simplified assay for measuring Toxoplasma gondii immunoglobulin G avidity. Clin Diagn Lab Immunol 2001; 8: 904–908.

    CAS  PubMed  PubMed Central  Google Scholar 

  8. Montoya GJ, Liesenfeld O, Kinney S, Press C, Remington SJ . VIDAS test for avidity of Toxoplasma-specific immunoglobulin G for confirmatory testing of pregnant women. J Clin Microbiol 2002; 40: 2504–2508.

    CAS  Article  Google Scholar 

  9. Eaton RB, Petersen E, Seppänen H, Tuuminen T . Multicenter evaluation of a fluorometric enzyme immunocapture assay to detect Toxoplasma-specific immunoglobulin M in dried blood filter paper specimens from newborns. J Clin Microbiol 1996; 34: 3147–3150.

    CAS  PubMed  PubMed Central  Google Scholar 

  10. Gilbert RE, Thalib L, Tan HK, Paul M, Wallon M, Petersen E . European multicentre study on congenital toxoplasmosis. Screening for congenital toxoplasmosis: accuracy of immunoglobulin M and immunoglobulin A tests after birth. J Med Screen 2007; 14: 8–13.

    Article  Google Scholar 

  11. Harry E, Prince MW . Simplified assay for measuring Toxoplasma gondii immunoglobulin G avidity. Clin Diag Lab Immunol 2001; 8: 904–908.

    Google Scholar 

  12. Fescina R, Simini F, Belitsky R . Difusión: Evaluación de los procedimientos diagnósticos, aspectos metodológicos. Sal Perinat 1985; 2: 39–44.

    Google Scholar 

  13. Montoya JG, Remington JS . Management of Toxoplasma gondii infection during pregnancy. Clin Infect Dis 2008; 47: 554–566.

    Article  Google Scholar 

  14. De Paschale M, Agrappi C, Belvisi L, Cagnin D, Cerulli T, Clerici P, Mirri P, Manco MT, Cavallari S, Viganò EF . Revision of the positive predictive value of IgM anti-Toxoplasma antibodies as an index of recent infection. New Microbiol 2008; 31: 105–111.

    PubMed  Google Scholar 

  15. Lefevre-Pettazzoni M, Le Cam S, Wallon M, Peyron F . Delayed maturation of immunoglobulin G avidity: implication for the diagnosis of toxoplasmosis in pregnant women. Eur J Clin Microbiol Infect Dis 2006; 25: 687–693.

    CAS  Article  Google Scholar 

Download references

Acknowledgements

This work was partially financed by CONACYT, Mexico, Grants 43079 and 69666. The English spelling and grammar was reviewed by Bachelor in English Language and Literature Alfonso Fernández-Portilla.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to D Correa.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Cañedo-Solares, I., Ortiz-Alegría, L., Figueroa-Damián, R. et al. Toxoplasmosis in pregnancy: determination of IgM, IgG and avidity in filter paper-embedded blood. J Perinatol 29, 668–672 (2009). https://doi.org/10.1038/jp.2009.79

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/jp.2009.79

Keywords

  • avidity
  • IgM
  • IgG
  • newborns
  • paper filter-embedded blood
  • pregnant women

Further reading

Search

Quick links