Original Article | Published:

Plasma expression level of miRNA let-7 is positively correlated with carotid intima-media thickness in patients with essential hypertension

Journal of Human Hypertension volume 31, pages 843847 (2017) | Download Citation

Abstract

MicroRNAs (miRNA, miR) play vital part in the pathophysiology of arterial remodeling in hypertension patients, and are increasingly becoming novel biomarkers in cardiovascular disease. The study was designed to evaluate the correlation between let-7 expression level and subclinical atherosclerosis in untreated patients with newly diagnosed essential hypertension. We assessed 240 participants including 60 healthy volunteers with normal carotid intima-media thickness (nCIMT), 60 healthy volunteers with increased CIMT (iCIMT), 60 hypertension patients with nCIMT and 60 hypertension patients with iCIMT. All patients underwent measurements of CIMT and ambulatory blood pressure (BP) monitoring. The level of let-7 was quantified using real-time reverse transcription polymerase chain reaction. Correlations of let-7 expression with BP parameters and CIMT were assessed using multiple linear regression analysis. We observed the lowest miRNA let-7 expression (21.70±1.45 vs 29.33±2.58 vs 31.50±1.80 vs 35.49±2.33; P<0.001) in healthy controls with nCIMT, followed by healthy controls with iCIMT, then hypertension patients with nCIMT and highest expression in hypertension patients with iCIMT. Let-7 was independently correlated with CIMT(r=0.587, P<0.001), and multiple linear regression analysis showed that let-7 was independently correlated with CIMT (β=0.031, P<0.001). Our findings provide significant evidence that plasma let-7 could represent a non-invasive marker for atherosclerosis in hypertensive patients and herald the emergence of a potential indicator to monitor end-organ damage in hypertension.

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Acknowledgements

The authors thank all the participating volunteers for their efforts and contributions. This study was funded from Guangdong Natural Science Foundation (No. S2013010016575, No. 2015A030313660), Technology Project Foundation of Guangzhou (No. 2014y2-00140, No. 1563000381), Technology Project Foundation of Guangdong Province (No. 2014B020212008) and the National Natural Science Foundation of China (No. 81300230).

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Affiliations

  1. Department of Cardiology, Guangdong Cardiovascular Institute, Guangdong Provincial Key Laboratory of Coronary Heart Disease Prevention, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China

    • Y-q Huang
    • , C Huang
    • , J-y Chen
    • , J Li
    •  & Y-q Feng

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The authors declare no conflict of interest.

Corresponding author

Correspondence to Y-q Feng.

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DOI

https://doi.org/10.1038/jhh.2017.52

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