Conjunctival melanoma represents 5% of ocular melanomas. It is a highly recurrent tumor leading to metastatic disease and death at 10 years in 25–30% of patients.1 Recent molecular investigations have indicated that conjunctival and cutaneous melanomas may share common features.2 For instance, epidemiological studies have shown an association between decreasing latitude and increasing incidence of conjunctival melanoma,3 suggesting that exposure to sunlight has a role in its etiology.4 However, strong molecular proof of such correlation has not been established. We hereby report the results of a genome-wide sequencing effort of two conjunctival melanomas documenting a strong ultraviolet (UV) mutation signature.
We performed high-coverage whole-genome sequencing (average read depth=80x, with 97.1% of the genome covered at least 40x) of two untreated perilimbic conjunctival melanomas occurring in two Caucasian women, aged 57 years (patient A) and 65 years (patient B) (Figures 1a and b). Somatic mutations were ascertained by comparing the DNA sequence of each tumor with their respective germline matches (blood leukocyte DNA). We identified a very high somatic mutation load composed of more than 96 000 mutations in each tumor, resulting in an average genome-wide mutational rate of 30 somatic mutations per million DNA bases. On average, we detected 802 mutations in the coding regions of genes. The ratio of non-synonymous to synonymous variants was 1.59:1, showing a prominence of passenger mutations. Non-synonymous mutations were composed mainly of missense (90%), followed by nonsense changes (7%).
Moreover, detailed analysis of the mutation spectrum uncovered a majority of cytosine to thymine (C>T) transitions, occurring in 88 (patient A) and 83% (patient B) of the single-nucleotide substitutions in these tumors (Figure 1c). In addition, 80 and 100% of these C>T changes, respectively, occurred at the 3′ end of pyrimidine dinucleotides and CC>TT changes represented >70% of all tandem substitutions, showing the presence of a typical UV mutational signature.5
Altogether, our molecular findings support the occurrence of UV-induced DNA damage in conjunctival melanoma indicating a link between solar radiation and development of this tumor. Awareness should therefore be raised in the general population about the benefits of wearing UV-shielding eyeglasses, in addition to skin sunscreens, when exposed to sunlight.
References
Missotten, G. S., Keijser, S., De Keizer, R. J. & De Wolff-Rouendaal, D. Conjunctival melanoma in the Netherlands: a nationwide study. Invest. Ophthalmol. Vis. Sci. 46, 75–82 (2005).
Griewank, K. G., Westekemper, H., Murali, R., Mach, M., Schilling, B., Wiesner, T. et al. Conjunctival melanomas harbor BRAF and NRAS mutations and copy number changes similar to cutaneous and mucosal melanomas. Clin. Cancer Res. 19, 3143–3152 (2013).
Yu, G. P., Hu, D. N. & McCormick, S. A. Latitude and incidence of ocular melanoma. Photochem. Photobiol. 82, 1621–1626 (2006).
Griewank, K. G., Murali, R., Schilling, B., Scholz, S., Sucker, A., Song, M. et al. TERT promoter mutations in ocular melanoma distinguish between conjunctival and uveal tumours. Br. J. Cancer 109, 497–501 (2013).
Ikehata, H. & Ono, T. The mechanisms of UV mutagenesis. J. Radiat. Res. 52, 115–125 (2011).
Acknowledgements
This work was supported by the ‘Fond'action contre le cancer’ foundation, Switzerland. We wish to thank Complete Genomics (Mountain View, CA, USA) for providing the whole-genome sequencing discussed in this report.
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Rivolta, C., Royer-Bertrand, B., Rimoldi, D. et al. UV light signature in conjunctival melanoma; not only skin should be protected from solar radiation. J Hum Genet 61, 361–362 (2016). https://doi.org/10.1038/jhg.2015.152
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DOI: https://doi.org/10.1038/jhg.2015.152
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