Summary
The expression of histo-blood group Lewis antigens is determined by the Lewis-type α1→3/4fucosyltransferase (Le enzyme) encoded by Fuc-TIII gene (Le gene). The genotyping ofLe genes by the PCR-RFLP methods established recently and partly modified in this study was found to be useful not only for determining the genuine Lewis blood types of samples such as human hairs and blood stains but also for distinguishing non-genuine Lewis-negative phenotypes frequently observed in pregnant women from genuine ones. The availability of the present PCR-RFLP methods for the paternity tests was also discussed.
Similar content being viewed by others
Article PDF
References
American Association of Blood Banks (1981): Technical manual of the American Association of Blood Banks. 8th ed. Washington, DC, p 392
Ernst C, Atkinson B, Wysocka M, Blaszczyk M, Herlyn M, Sears H, Steplewsky Z, Koprowsky H (1984): Monoclonal antibody localization of Lewis antigens in fixed tissue. Lab Invest50: 394–400
Good AH, Yau O, Lamontagne LR, Oriol R (1992): Serological and chemical specificities of twelve monoclonal anti-Lea and anti-Leb antibodies. Vox Sang62: 180–189
Hammer L, Mansson S, Rohr T, Chester MA, Ginsburg V, Lundblad A, Zopf D (1981): Lewis-phenotype of erythrocytes and Leb active glycolipid in serum of pregnant women. Vox Sang40: 27–33
Hirano K, Kawa S, Oguchi H, Kobayashi T, Yonekura H, Ogata H, Homma T (1987): Loss of Lewis antigen expression on erythrocytes in some cancer patients with high serum CA19-9 levels. J Natl Cancer Inst79: 1261–1268
Kelly RJ, Rouquier S, Giorfi D, Lennon GG, Lowe JB (1995): Sequence and expression of a candidate for the human secretor blood group α(1,2)fucosyltransferase gene (FUT2). J Biol Chem270: 4640–4649
Kukowska-Latallo JF, Larsen RD, Nair RP, Lowe JB (1990): A cloned human cDNA determines expression of a mouse stage-specific embryonic antigen and the Lewis blood group α(1,3/1,4)fucosyltransferase. Genes Dev4: 1288–1303
Limas C (1986): Detection of urothelial Lewis antigens with monoclonal antibodies. Am J Pathol125: 515–523
Lutz P, Dzik WH (1992): Molecular biology of red cell blood group genes. Transfusion32: 467–483
Makni S, Dalix AM, Caillard T, Compagnon B, Pendu JL, Ayed K, Oriol R (1987): Discordance between red cell and saliva Lewis phenotypes in patients with hydatid cysts. Exp Clin Immunogenet4: 136–143
Nishihara S, Yazawa S, Iwasaki H, Nakazato N, Kudo T, Ando T, Narimatsu H (1993): α(1,3/1,4)Fucosyltransferase (FucT-III) gene is inactivated by a single amino acid substitution in Lewis histo-blood type negative individuals. Biochem Biophys Res Commun196: 624–631
Nishihara S, Narimatsu H, Iwasaki H, Yazawa S, Akamatsu S, Ando T, Seno T, Narimatsu I (1994): Molecular genetic analysis of the human Lewis histo-blood group system. J Biol Chem269: 29271–29278
Oriol R, Sammelsson BE, Messeter L (1990): ABO antibodies—serological behavior and immuno-chemical characterization. J Immunogenet17: 279–299
Ørntoft TF, Holmes H, Johnson J, Hakomori S, Clausen H (1991): Differential tissue expression of the Lewis blood antigens: enzymatic, immunohistologic, and immunochemical evidence for Lewis a and b antigen expression in Le(a−b−) individuals. Blood77: 1389–1396
Rouquier S, Lowe JB, Kelly J, Fertitta AL, Lennon GG, Giorgi D (1995): Molecular cloning of a human genomic region containing the H blood group α(1,2)fucosyltransferase gene and two H locus-related DNA restriction fragments. J Biol Chem270: 4632–4639
Sabrock J, Fritsch EF, Maniatis T (1992) Molecular cloning: A laboratory manual. 2nd ed., Cold, Spring Harbor Laboratory Press, New York, 2.80
Schachter H (1994): Molecular cloning of glycosyltransferase genes. In: Fukuda M, Hindusgaul O (eds). Molecular glycobiology, IRL Press, New York, pp 88–162
Stigendal L, Olsson R, Rydberg L, Samuelsson BE (1984): Blood group Lewis phenotype on erythrocytes and in saliva in alcoholic pancreatitis and chronic liver disease. J Clin Pathol37: 778–786
Watkins WM (1980): Biochemistry and genetics of the ABO, Lewis and P blood group system. Adv Hum Genet10: 1–136
Yazawa S, Asao T, Izawa H, Miyamoto Y, Matta KL (1988): The presence of CA19-9 in serum and saliva from Lewis blood-group negative cancer patients. Jpn J Cancer Res79: 538–543
Yazawa S, Madiyalakan R, Jain RJ, Shimoda N, Matta KL (1990a): Use of benzyl 2-acetamido-2-deoxy-3-O-(2-O-methyl-β-d-galactosyl)-β-d-glucopyranoside [2′-O-methyllacto-N-biose IβBn] as a specific acceptor for GDP-fucose:N-acetylglucosaminide α(1→4)-l-fucosyltransferase. Anal Biochem187: 374–378
Yazawa S, Kochibe N, Asao T (1990b): A simple procedure for isolation of tumor-associated antigens by affinity chromatography using fucose-specificAleuria aurantia lectin. Immunol Invest19: 319–327
Yazawa S, Takeya A, Hosomi O, Nakajima T, Shimoda N, Ohi H, Tachikawa T, Piscorz CF, Jain RK, Matta KL (1992): Use of synthetic H-disaccharides as acceptors for detecting activities of UDP-GalNAc:Fucα1→2Galβ-R α1→3-N-acetylgalactosaminyltransferase in plasma samples from blood group A subgroups. Clin Chem38: 2392–2395
Yazawa S, Akamatsu S, Tachikawa T Naito H, Nakamura J, Asao T, Nagamachi Y, Nakajima T, Shin S, Chia D (1993): Development and characterization of a novel anti-fucosylated antigen monoclonal antibody YB-2 and its usefulness in the immunohistochemical diagnosis of colorectal cancer. Jpn J Cancer Res84: 641–648
Yazawa S, Nishihara S, Iwasaki H, Asao T, Nagamachi Y, Matta KL, Narimatsu H (1995): Genetic and enzymatic evidence for Lewis enzyme expression in Lewis-negative cancer patients. Cancer Res55: 1473–1478
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yazawa, S., Oh-kawara, H., Nakajima, T. et al. Histo-blood group lewis genotyping from human hairs and blood. Jap J Human Genet 41, 177–188 (1996). https://doi.org/10.1007/BF01892625
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01892625