Summary
To obtain cosmid markers and transcribed sequences from a specific chromosome region, a series of radiation-reduced hybrids (RHs) containing various regions of human chromosome 11 was prepared from microcell hybrid A9 (neo11) cells containing a normal human chromosome 11 tagged with pSV2neo at 11p11.2. Among 15 radiation hybrid clones isolated, RH(11)-9 which contains a q23 fragment in addition to theneo integration site, was used for the construction of a cosmid library. Cosmid clones having human DNA sequences were screened, and localized by Southern hybridization with the radiation hybrid panel. Fifty-nine cosmids were assigned to 11q23 and 6 cosmids to 11p11.2. Exon amplification proceeded with 23 of the 59 cosmids and 16 putative exons were cloned. Three of them were identical to those constituting a known gene which locates on q23 (ATDC), and the others were unknown. Thus, the RHs containing various subchromosomal fragments of chromosome 11 were useful for constructing region-specific DNA markers. The RH-(11)-9 cells and putative exons also facilitate the positional cloning of genes in the 11q23 region.
Similar content being viewed by others
Article PDF
References
Akao Y, Seto M, Yamamoto K, Iida S, Nakazawa S, Inazawa J, Abe T, Takahashi T, Ueda R (1992): The RCK gene associated with t(11;14) translocation is distinct from the MLL/ALL-1 gene with t(4;11) and t(11;19) translocation. Cancer Res52:6083–6087
Buckler AJ, Chang DD, Graw SL, Brook D, Haber DA, Sharp PA, Housman DE (1991): Exon amplification: a strategy to isolate mammalian genes based on RNA splicing. Proc Natl Acad Sci USA88:4005–4009
Call KM, Glaser T, Ito CY, Buckler AJ, Pelletier J, Haber DA, Rose EA, Kral A, Yeger H, Lewis WH, Jones C, Housman DE (1990): Isolation and characterization of a zinc finger polypeptide gene at the human chromosome 11 Wilms' tumor locus. Cell60:509–520
Carter SL, Negrini M, Baffa R, Gillum DR, Rosenberg AL, Schwartz GF, Croce CM (1994): Loss of heterozygosity at 11q22-q23 in breast cancer. Cancer Res54:6270–6274
Chen Z, Brand NJ, Chen A, Chen S-J, Tong J-H, Wang Z-Y, Waxman S, Zelent A (1993): Fusion between a novel Kruppel-like zinc finger gene and retinoic acid receptor-α locus due to a variant t(11:17) translocation associated with acute promyelocytic leukemia. EMBO J12:1161–1167
Cherif D, Der-Sarkissan H, Derre J, Tokino T, Nakamura Y, Berger R (1992): The 11q23 breakpoint in acute leukemia with t(11;19)(q23;p13) is distal to those of t(4;11), t(6;11) and t(9;11). Genes Chromosomes Cancer4:107–112
Chomczynski P, Sacchi N (1987): Single-step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction. Anal Biochem162:156–159
Devilee P, Van Schothorst EM, Bardoel AFJ, Bonsing B, Kuipers-Dijkshoorn N, James MR, Freuren G, Van der may AGL, Cornelisse CJ (1994): Allelotype of head and neck paragangliomas: allelic imbarance is confined to the long arm of chromosome 11, the site of the predisposing locus PGL. Genes Chromosomes Cancer11:71–78
Emi M, Takahashi E, Koyama K, Okui K, Oshimura M, Nakamura Y (1992): Isolation and mapping of 88 new RFLP markers on human chromosome 8. Genomics13:1261–1266
Foulkes WD, Champbell IG, Stamp GW, Trowsdale J (1993): Loss of heterozygosity and amplification on chromosome 11q in human ovalian cancer. Br J Cancer67:268–273
Gatti R (1991): Localizing the genes for Ataxia-telangiectasia: a human models for inherited cancer susceptibility. Adv Cancer Res56:77–104
Gerhard DS, Lawrence E, Wu J, Chua H, Ma N, Bland S, Jones C (1992): Isolation of 1001 new markers from human chromosome 11, excluding the region of 11q13-p15.5, and their sublocalization by a new series of radiation-reduced somatic cell hybrids. Genomics13:1133–1142
Gillet GT, McConville CM, Byrd PJ, Stankovic T, Taylor AM, Hunt DM, West LF, Fox MF, Povey S, Benham F (1993): Irradiation hybrids for human chromosome 11: characterization and use for generating region-specific markers in 11q14-q23. Genomics15:332–341
Hampton GM, Penny LA, Baergen RN, Larson A, Brewer C, Liao S, Busby-Earle RMC, Williams AWR, Steel CM, Bird CC, Stanbridge EJ, Evans GA (1994): Loss of heterozygosity in cervical carcinoma: subchromosomal localization of a putative tumor-suppressor gene to chromosome 11q22-q24. Proc Natl Acad Sci USA91:6953–6957
Heutink P, Van der May AGL, Sandkuijl LA, Van Gils APG, Bardoel A, Breedvald GJ, Van Vliet M, Van Ommen GJB, Cornelisse CJ, Oostra BA, Weber JL, Devilee P (1992): A gene subject to genomic imprinting and responsible for hereditary paragangliomas maps to chromosome 11q23-qter. Hum Mol Genet1:7–10
Hori T, Takahashi E, Tanigami A, Tokino T, Nakamura Y (1992): A high-resolution cytogenetic map of 186 cosmid DNA markers for human chromosome 11. Genomics13:129–133
Kapp LN, Painter RB, Yu LC, Van Loon N, Richard CW III, James MR, Cox DR, Murane JP (1992): Cloning of a candidate gene for Ataxia-telangiectasia group D. Am J Hum Genet51: 45–54
Keldysh PL, Dragani TA, Fleischman EW, Konstantinova LN, Perevoschikov AG, Pierotti MA, Della Porta G, Kopnin BP (1993): 11q deletions in human colorectal carcinomas: cytogenetics and RFLP analysis. Genes Chromosomes Cancer6:45–50
Kobayashi H, Episona R III, Fernand AA, Bogy C, Diaz MO, LeBeou MM, Rowly JD (1993): Analyses of deletions of the long arm of chromosome 11 in hematologic malignancies withFISH. Genes Chromosomes Cancer8:246–252
Koi M, Shimizu M, Morita H, Yamada H, Oshimura M (1989): Construction of mouse A9 clones containing a single chromosome tagged with neomycin-resistance genevia microcell fusion. Jpn J Cancer Res80:413–418
Kugoh H, Nakagawa Y, Mitsuya K, Mita T, Suzuki M, Suzuki N, Uejima H, Yuasa Y, Oshimura M (1995): Isolation and mapping of 186 new DNA markers on human chromosome 1. Genomics27:207–210
Kurimasa A, Nagata Y, Shimizu M, Emi M, Nakamura Y, Oshimura M (1994): A human gene that restores the DNA-repair defect in SCID mice is located on 8p11.1-q11.1. Hum Genet93:21–26
Leach RJ, Thayer MJ, Chafer AJ, Fournier REK (1989): Physical mapping of human chromosome 17 using fragment-containing microcell hybrids. Genomics5:167–176
Ledbetter SA, Nelson DL, Warren ST, Ledbetter DH (1990): Rapid isolation of DNA probes within chromosome regions by interspersed repetitive sequence polymerase chain reaction. Genomics6:475–481
Leonhardt EA, Kapp LN, Young BR, Murane JP (1994): Cloning of a candidate gene for Ataxiatelangiectasia group D. Genomics19:130–136
Monaco AP, Neve RL, Colleti-Feener C, Bertelson CJ, Kurnit DM, Kunkel LM (1986): Isolation of candidate cDNAs for portions of the Duchenne muscular distrophy gene. Nature323:646–650
Page DC, Mosher R, Simpson EM, Fisher EMC, Mardon G, Pollac J, McGillivray B, de la Chapelle A, Brown LG (1987): The sex-determining region of the human Y chromosome encodes a finger protein. Cell51:1091–1104
Rabbits TH (1994): Chromosomal translocations in human cancer. Nature372:134–149
Rommens JM, Iannuzzi MC, Kerem BS, Drumm ML, Melmer G, Dean M, Rozmahel R, Cole JL, Kennedy D, Hidaka N, Buchwald M, Riordan JR, Tsui LC, Collins FS (1989): Identification of the cystic fibrosis gene: chromosome walking and jumping. Science245:1059–1065
Sanger F, Nicklen S, Coulson AR (1977): DNA sequencing with chain termination inhibitors. Proc Natl Acad Sci USA74:5463–5467
Sobel E, Lange E, Jaspers NGJ, Chessa L, Sanal O, Shiloh Y, Taylor AMR, Weemaes CM, Lange K, Gatti RA (1992): Ataxia-telangiectasia: linkage evidence for genetic heterogeneity. Am J Hum Genet50:1343–1348
Takahashi E, Hori T, O'Connell P, Leppert M, White R (1990): R-banding and nonisotpoicin situ hybridization: precise localization of the human type II collagen (Co12A1). Hum Genet86:14–16
Takahashi E, Yamakawa K, Nakamura Y, Hori T (1992): A high resolution cytogenetic map of human chromosome 3: localization of 291 new cosmid markers by direct R-banding fluorescencein situ hybridization. Genomics13:1047–1055
Tamari M, Hamaguchi M, Shimizu M, Oshimura M, Takayama H, Kohno T, Yamaguchi N, Sugimura T, Terada M, Yokota J (1992). Ordering of human chromosome 3p markers by radiation hybrid mapping. Genomics13:705–712
Tokino T, Takahashi E, Mori M, Tanigami A, Glaser T, Park JW, Jones C, Hori T, Nakamura Y (1991): Isolation and mapping of 62 new RFLP markers on human chromsome 11. Am J Hum Genet48:258–262
Tomlinson IPM, Gammack AJ, Sticland JE, Mann GJ, Mackie RM, Kefford RF, McGee JO (1993): Loss of heterozygosity in malignant melanoma at loci on chromosome 11 and 17 implicated in the pathogenesis of other cancers. Genes Chromosomes Cancer7:169–172
Ziemin S, McCabe NR, Gill HJ, Episona R III, Patel Y, Harden A, Rubinelli P, Smith SD, LeBeau MM, Rowley JD, Diaz MO (1991). Identification of a gene, MLL, that spans the breakpoint in 11q23 translocations associated with human leukemias. Proc Natl Acad Sci USA88:10735–10739
Zoghbi HY, McCall AE, LeBorgne-Demarquoy F (1991): Sixty-five radiation hybrids for the short arm of human chromosome 6: their value as a mapping panel and a source for rapid isolation of new probes using repeat element-mediated PCR. Genomics9:713–720
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Katoh, M., Nakagawa, Y., Yawata, T. et al. Cosmids and transcribed sequences from chromosome 11q23. Jap J Human Genet 40, 307–317 (1995). https://doi.org/10.1007/BF01900597
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF01900597