Summary
We have constructed a new genetic linkage map of the Werner syndrome (WRN) region, using microsatellites from a library which was developed by a chromosome microdissection and enzymatic amplification method. These microsatellites were used to genotype members of CEPH families using a simplified detection system of polymerase chain reaction (PCR) products. Two-point analysis was used to assign 4 microsatellite markers relative to each marker and other markers reported in the CEPH public data base. We confirmed that these 4 markers are located to the WRN region, 8p11.2–p22. Such microsatellites microdissected from the definite chromosome region may be useful for positional cloning.
Similar content being viewed by others
Article PDF
References
Deng H-X, Yoshiura K, Dirks RW, Harada N, Hirota T, Tsukamoto K, Jinno Y, Niikawa N (1992): Chromosome-band-specific painting: Chromosomein situ suppression hybridization using PCR products from a microdissected chromosome band as a probe pool. Hum Genet89: 13–17
Goto M, Rubinstein M, Weber J, Woods K, Drayna D (1992): Genetic linkage of Werner's syndrome to five markers on chromosome 8. Nature335: 735–738
Hirota T, Tsukamoto K, Deng H-X, Yoshiura K, Ohta T, Tohma T, Kibe T, Harada N, Jinno Y, Niikawa N (1992): Microdissection of human chromosomal regions 8q23.3–q24.11 and 2q33-qter: construction of DNA libraries and isolation of their clones. Genomics13: 349–354
Jinno Y, Harada N, Yoshiura K, Ohta T, Hirota T, Tsukamoto K, Deng H-X, Oshimura M, Niikawa N (1992): A simple and efficient amplification method of DNA with unknown sequences and its application to microdissection/microcloning. J Biochem112: 75–80
Kamino K, Nakura J, Kihara K, Lin Ye, Nagano K, Miki T, Ohta T, Jinno Y, Niikawa N, Ogihara T (1993). Population variation in the dinucleotide repeat polymorphism at the D8S360 locus. Hum Mol Genet2: 1751
Kibe T, Tsukamoto K, Hirota T, Yoshiura K, Ohta T, Tohma T, Tamura T, Jinno Y, Niikawa N (1992): Isolation of 2 novel RFLP markers and their localization at 2q35 by microdissection and subsequent enzymatic amplification. Jpn J Human Genet37: 263–270
Lander ES, Botstein D (1987): Homozygosity mapping: A way to map human recessive traits with the DNA of inbred children. Science236: 1569–1570
Lathrop GM, Lalouel JM, Julier C, Ott J (1985): Multilocus linkage analysis in humans: Detection of linkage and estimation of recombination. Am J Hum Genet37: 482–498
Schellenberg DG, Martin GM, Wijsman EM, Nakura J, Miki T, Ogihara T (1992): Homozygosity mapping and Werner's syndrome. Lancet339: 1002
Tomfohrde J, Woods S, Schertzer M, Wagner MJ, Wells DE, Parrish L, Salder LA, Blanton SH, Daiger SP, Wang Z, Wilkie PJ, Weber JL (1992): Human chromosome 8 linkage map based on short tandem repeat polymorphisms: Effect of genotype errors. Genomics14: 144–152
Weissenbach J, Gyapay G, Dib C, Viginal A, Morissentte J, Millasseau P, Vaysseix G, Lathrop M (1992): A second-generation linkage map of the human genome. Nature359: 794–801
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nagano, K., Nakura, J., Kihara, K. et al. Isolation and mapping of microsatellites from a library microdissected from the Werner syndrome region, 8p11.2–p22. Jap J Human Genet 38, 391–397 (1993). https://doi.org/10.1007/BF01907985
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF01907985
Key Words
This article is cited by
-
Two dinucleotide repeat polymorphisms at the D8S1218 and D8S1219 loci
Japanese journal of human genetics (1996)
-
Two dinucleotide repeat polymorphisms at the D8S1444 and D8S1445 loci
Japanese journal of human genetics (1996)
-
Dinucleotide repeat polymorphism at the D8S1223 locus
Japanese journal of human genetics (1996)
-
Dinucleotide repeat polymorphism at the D8S1054
Japanese journal of human genetics (1995)
-
Two dinucleotide repeat polymorphisms at the D8S1442 and D8S1443 loci
Japanese journal of human genetics (1995)