Summary
We developed a simple and efficient procedure for the establishment of Epstein-Barr virus-transformed human lymphoblastoid cell lines. B-lymphocytes were obtained by centrifugation after hemolysis of red cells with a hemolysis buffer, instead of Ficoll-Parque gradient. We can start a primary culture within 15 min by using this method.
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References
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Fukushima, Y., Ohashi, H., Wakui, K. et al. A rapid method for starting a culture for the establishment of Epstein-Barr virus-transformed human lymphoblastoid cell lines. Jap J Human Genet 37, 149–150 (1992). https://doi.org/10.1007/BF01899737
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DOI: https://doi.org/10.1007/BF01899737
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