Summary
The nucleotide sequence of a HLA-DRB gene with a predominant subtype of DRw8 specificity in Japanese (DR8.1) was determined with single-stranded DNA enzymatically amplified by polymerase chain reaction (PCR). The sequence differs at a single amino acid from both of the published DRw8/Dw8.1 and DRw8/Dw8.2 sequences: isoleucine67(ATC) instead of phenylalanine67(TTC) in DRw8/Dw8.1 and serine57(AGC) instead of aspartic acid57(GAT) in DRw8/Dw8.2. On the other hand the DR8.1 and DRw8/Dw8.3 have the same amino acid sequence although one silent nucleotide substitution has occurred between the two sequences. These results indicate that Japanese DR8.1 specificity corresponds to DRw8/Dw8.3. Furthermore, an oligonucleotide probe specific for this sequence was synthesized and hybridized with 33 HLA-typed controls. This probe clearly distinguished the particular subtype from other DRw8 subtypes and specificities.
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Bell, J.I., Denney, D., Foster, L., Belt, T., Todd, J.A. and McDevitt, H.O. 1987. Allelic variation in the DR subregion of the human major histocompatibility complex.Proc. Natl. Acad. Sci. U.S.A. 84: 6234–6238.
Bétuel, H., Gebuhrer, L., Schreuder, G.M.T., Layrisse, Z., Arnaiz-Villena, A. and Goldman, S.F. 1984. HLA-DRw8 antigen report. InHistocompatibility Testing 1984, Albert, E.D., Bauer, M.P. and Mayr, W.R., eds. Springer-Verlag, Berlin, Heidelberg, pp. 198–200.
Böhme, J., Andersson, M., Andersson, G., Moeller, E., Peterson, P.A. and Rask, L. 1985. HLA-DRβ genes vary in number between different DR specificities, whereas the number of DQβ genes is constant.J. Immunol. 135: 2149–2155.
Bouillot, M., Choppin, J., Sterkers, G., Friedel, C., Gebuhrer, L., Betuel, H. and Levy, P. 1988. The two-dimensional migration pattern of the single HLA-DR beta chain expressed in DRw8 haplotypes is not fully predictive of its activity in antigen presentation.Immunogenetics 28: 46–49.
Gorski, J. 1989. The HLA-DRw8 lineage was generated by a deletion in the DR B region followed by first domain diversification.J. Immunol. 142: 4041–4045.
Grosse-Wilde, H., Doxiadis, I. and Brandt, H. 1984. Definition of HLA-D with HTC. InHistocompatibility Testing 1984, Albert, E.D., Baur, M.P. and Mayr, W.R., eds., Springer-Verlag, Berlin, Heidelberg, pp. 249–264.
Gyllensten, U.B. and Erlich, H.A. 1988. Generation of single-stranded DNA by the polymerase chain reaction and its application to direct sequencing of the HLA-DQA locus.Proc. Natl. Acad. Sci. U.S.A. 85: 7652–7656.
Jonsson, A.-K., Andersson, L. and Rask, L. 1989. A cellular and functional split in the DRw8 haplotype is due to a single amino acid replacement (DRβser57-asp57).Immunogenetics 29: 308–316.
Layrisse, Z., Carreño, B., Svejgaard, A., Platz, P., Ryder, L.P. and Kristensen, T. 1984. First level testing of Dw8 HTC. InHistocompatibility Testing 1984, Albert, E.D., Baur, M.P. and Mayr, W.R., eds., Springer-Verlag, Berlin, Heiderlberg, pp. 278–281.
Long, E.O., Wake, C.T., Gorski, J. and Mach, B. 1983. Complete sequence of an HLA-DRβ chain deduced from a cDNA clone and identification of multiple non-allelic DRβ chain genes.EMBO J. 2: 389–394.
Mickelson, E.M., Nisperos, B., Layrisse, Z., Kim, S.J., Thomas, E.D. and Hansen, J.A. 1983. Analysis of the HLA-DRw8 haplotype: recognition by HTC typing of three distinct antigen complexes in Caucasians, native Americans, and Orientals.Immunogenetics 17: 399–410.
Navarrete, C., Seki, T., Miranda, A., Winchester, R. and Gregersen, P.K. 1989. DNA sequence analysis of the HLA-DRw12 allele.Hum. Immunol. 25: 51–58.
Todd, J.A., Bell, J.I. and McDevitt, H.O. 1987. HLA-DQβ gene contributes to susceptibility and resistance to insulin-dependent diabetes mellitus.Nature 329: 599–604.
Tokunaga, K., Kay, P.H., Christiansen, F.T., Saueracker, G. and Dawkins, R.L. 1989. Comparative mapping of the human major histocompatibility complex in different racial groups by pulsed field gel electrophoresis.Hum. Immunol. 26: 91–97.
Watanabe, Y., Tokunaga, K., Matsuki, K., Takeuchi, F., Matsuta, K., Maeda, H., Omoto, K. and Juji, T. 1989. Putative amino acid sequence of HLA-DRB chain contributing to rheumatoid arthritis susceptibility.J. Exp. Med. 169: 2263–2268.
WHO-HLA Nomenclature Committee 1988. Nomenclature for factors of the HLA system, 1987.Vox Sang. 55: 119–126.
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Watanabe, Y., Tokunaga, K., Matsuki, K. et al. Direct sequencing of a HLA-DRB gene by polymerase chain reaction: Sequence variation in DRw8 specificity. Jap J Human Genet 35, 151–157 (1990). https://doi.org/10.1007/BF01876460
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DOI: https://doi.org/10.1007/BF01876460
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