Summary
A polymorphic human DNA fragment, OS-4, isolated from pBR322 human genomic library was mapped to chromosome 18 using a human-mouse somatic cell hybrid panel. More precise assignment of this locus to 18q21.3→qter was made by hybridization to DNA from six cell lines containing different structural abnormalities of chromosome 18.
In addition toTaqI polymorphism, it was proved that OS-4 could detect polymorphism inPstI-digested human DNA. This probe, designated as D18S5, would be a useful marker for gene mapping as well as linkage analysis of genetic diseases.
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Botstein, D., White, R.L., Skolnick, M., and Davis, R.W. 1980. Construction of a genetic linkage map in man using restriction fragment length polymorphisms.Am. J. Hum. Genet. 32:314–331.
Cavenee, W., Leach, R., Mohandas, T., Pearson, P., and White, R. 1984. Isolation and regional localization of DNA segments revealing polymorphic loci from human chromosome 13.Am. J. Hum. Genet. 36:10–24.
Gusella, J.F., Wexler, N.S., Conneally, P.M., Naylor, S.L., Anderson, M.A., Tanzi, R.E., Watkins, P.C., Ottina, K., Wallace, M.R., Sakaguchi, A.Y., Young, A.B., Shoulson, I., Bonilla, E., and Martin, J.B. 1983. A polymorphic DNA marker genetically linked to Huntington's disease.Nature 306:234–238.
Human Gene Mapping 8, Helsinki Conference (1985), Eighth International Workshop on Human Gene Mapping. 1985.Cytogenet. Cell Genet. 40:360–489.
Ikeuchi, T. and Naito, T. 1982. A case of 18-trisomy syndrome showing a karyotype 46,XX,psu dic(18) (qter→cen→p11.32::p11.32→qter).Jpn. J. Human Genet. 27:222.
Nishisho, I., Miki, T., Tateishi, H., Takai, S., Motomura, K., Nakura, J., Kumahara, Y., Mori, T., and Honjo, T. 1986. Isolation of DNA clones revealing restriction fragment length polymorphisms in the human genome.Jpn. J. Human Genet. 31:249–258.
Reeders, S.T., Breuning, M.H., Davis, K.E., Nicholls, R.D., Jarman, A.P., Higgs, D.R., Pearson, P.L., and Weatherall, D.J. 1985. A highly polymorphic DNA marker linked to adult polycystic kidney disease on chromosome 16.Nature 317:542–544.
Shinohara, T., Ikeuchi, T., Asano, K., Hashimoto, K., Ueda, Y., and Ogoshi, T. 1985. A case of partial 18q trisomy resulting from a pericentric inversion of maternal chromosome 18.Jpn. J. Human Genet. 30:160–161.
Southern, E.M. 1975. Detection of specific sequences among DNA fragments separated by gel electrophoresis.J. Mol. Biol. 98:503–517.
Tateishi, H., Nishisho, I., Miki, T., Takai, S., and Honjo, T. 1986. An anonymous single copy chromosome 18 probe associated with a frequent RFLP.Nucleic Acids Res. 14:1926.
White, R., Woodward, S., Leppert, M., O'connell, P., Hoff, M., Herbst, J., Lalouel, J.-M., Dean, M., and Woude, G.V. 1985. A closely linked genetic marker for cystic fibrosis.Nature 318:382–384.
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Nishisho, I., Tateishi, H., Motomura, K. et al. Assignment of a polymorphic locus of OS-4(D18S5) DNA segment to human chromosome region 18q21.3→qter. Jap J Human Genet 32, 1–7 (1987). https://doi.org/10.1007/BF01876521
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DOI: https://doi.org/10.1007/BF01876521
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