Summary
Changes in the rate of semi-conservative DNA synthesis with time after ultraviolet light irradiation were measured with amniotic fluid cells at 6 to 10th subcultures, which were originally derived from the pregnancy with the fetus affected with Cockayne syndrome. The rate of DNA synthesis at 12 hr after ultraviolet exposure relative to that of unirradiated control was much less in the Cockayne amniotic fluid cells than those observed with normal amniotic fluid cells or with fibroblasts from normal donors. These results suggest that by measuring the rate of DNA synthesis prenatal diagnosis of Cockayne syndrome may be accomplished within 3 to 4 weeks, in contrast to the actual length of time, 5 weeks, required for the prenatal diagnosis of this syndrome by measuring colony forming ability of ultraviolet irradiated cells.
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Kawai, K., Ikenaga, M., Ohtani, H. et al. Rapid procedures for prenatal diagnosis of Cockayne syndrome. Jap J Human Genet 28, 223–229 (1983). https://doi.org/10.1007/BF01876604
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DOI: https://doi.org/10.1007/BF01876604
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