Genetic variants of the human diaphorase DIA3 in Japanese: Report of a new rare allele, DIA3 4

Article metrics

Summary

Sperm-lysates from 264 unrelated Japanese males were tested for the polymorphism of the human diaphorase DIA3 by isoelectric focusing in thin-layer polyacrylamide gel over pH range 3.5–9.5 gradient. The occurrence of a new rare allele, DIA3 4, in addition to the previously described three alleles in German and British populations; has been demonstrated in our population. The estimated allele frequencies of the commonly encountered phenotypes,i.e. DIA31–1, 2–1, 3–1, 2–2 and 3–2, were: DIA3 1=0.837, DIA3 2=0.143 and DIA3 3=0.020.

References

  1. Black, E.T. and Sensabaugh, G.F. 1978. Genetic markers in human Semen. 11. Quantitation of polymorphic proteins.J. Forensic Sci. 23: 717–729.

  2. Caldwell, K., Black, E.T., and Sensabaugh, G.F. 1976. Sperm diaphorase: Genetic polymorphism of a sperm-specific enzyme in man.Science 191: 1185–1187.

  3. Edwards, Y.H., Potter, J.E., and Hopkinson, D.A. 1979. A comparison of biochemical properties of the human diaphorase (DIA3) isozymes determined by the common alleles DIA3 1, DIA3 2 and DIA3 3.Ann. Hum. Genet. 42: 293–302.

  4. Fisher, T.A., Edwards, Y.H., Putt, W., Potter, J., and Hopkinson, D.A. 1977. An interpretation of human diaphorase isozymes in terms of three gene loci DIA1, DIA2 and DIA3.Ann. Hum. Genet. 41: 139–149.

  5. Kühnl, P., Langanke, U., Spielmann, W., and Neubauer, M. 1977. Investigations on the polymorphism of sperm diaphorase in man.—Evidence for a third common allele, SD3.Hum. Genet. 40: 79–86.

  6. Sebetan, I.M. and Akaishi, S. 1981. Alpha-1-antitrypsin (PiM) subtypes in Japanese.Forensic Sci. Int. 18: 155–159.

  7. Sebetan, I.M., Kudo, T., Hiraiwa, K., Ohno, Y., Kuroda, F., and Akaishi, S. 1982. Isoelectric focusing studies on the PGM1 subtypes in the northern Japanese population.Z. Rechtsmed. in press.

  8. Suyama, H., Nakasono, I., and Ohya, I. 1979. The distribution of common phenotype of sperm diaphorase in the Japanese.Forensic Sci. Int. 13: 125–127.

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Keywords

  • Rare Allele
  • Common Allele
  • Minor Zone
  • Estimate Allele Frequency
  • Common Phenotype

Further reading