In order to investigate the properties of the trisomic cells in patients with Down's syndrome, comparisons of the cell cycle, durations of component phases and chromosomal sensitivities were performed both in trisomic and normal lymphocyte cultures, using3H-thymidine autoradiography. In the present experiments, the presence of the two cell sub-populations was found in trisomic cells as well as in normal cells.
The durations of the G2+M and S phases were similar in both subpopulations, 3.9 and 8.1 hr for trisomic cells and 4.6 and 7.9 hr for normal cells, respectively. For the longer cell cycle sub-population, the duration of the total cell cycle and the G1 phase were 23.1 and 11.1 hr for the trisomic cells and 20.4 and 7.9 hr for the normal controls. For the shorter cell cycle subpopulation, those were 13.2 and 1.3 hr for the trisomic cells and 12.7 and 0.8 hr for the normal controls, respectively.
Chromosomal sensitivities to incorporated3H-TdR were similar in two sub-populations within a group, but significantly higher chromosome aberration yields were observed in the trisomic cells.
Beek, B., and Obe, G. 1974. The human leukocyte test system. II. Different sensitivities of subpopulations to a chemical mutagen.Mutat. Res. 24: 395–398.
Bender, M. A. and Brewen, J. G. 1969. Factors influencing chromosome aberration yields in the human peripheral leukocyte system.Mutat. Res. 8: 383–399.
Dudin, G., Beek, B. and Obe, G. 1974. The human leukocyte test system. I. DNA synthesis and mitosis in PHA-stimulated 2-day cultures.Mutat. Res. 23: 279–281.
Hayakawa, M., Matsui, I., Higurashi, M. and Kobayashi, N. 1968. Hyperblastic response to dilute P.H.A. in Down's syndrome.Lancet i: 95–96.
Higurashi, M., Tamura, T. and Nakatake, T. 1973. Cytogenetic observations in cultured lymphocytes from patients with Down's syndrome and measles.Pediat. Res. 7: 582–587.
Higurashi, M., Tada, A., Miyahara, S., Hirayama, M., Hoshina, H. and Tamura, T. 1976. Chromosome damage in Down's syndrome induced by chikinpox infection.Pediat. Res. 10: 189–192.
Kukharenko, V. I., Kuliev, A. M., Grinberg, K. N. and Terskikh, V. V. 1974. Cell cycles in human diploid and aneuploid strains.Humangenetik 24: 285–296.
Matte, R., Sasaki, M. and Obara, Y. 1969. Blastic responce of lymphocytes in mosaic Down's syndrome under serial PHA dilutions.Jap. J. Human Genet. 14: 160–162.
Michalowski, A. 1963. Time course of DNA synthesis in human leukocyte cultures.Exp. Cell Res. 32: 609–612.
O'Brien, R. L., Poon, P., Kline, E. and Parker, J. W. 1971. Susceptibility of chromosomes from patients with Down's syndrome to 7,12-dimethylbenz(a)anthracene-induced aberrationsin vitro.Int. J. Cancer 8: 202–210.
Okey, C. H. 1967. Chromatid aberrations resulting from3H-thymidine incorporation into early and late S period in human fibroblasts.Int. J. Radiat. Biol. 13: 479–484.
Paton, G. R., Silver, M. F. and Allison, A. C. 1974. Comparison of cell cycle time in normal and trisomic cells.Humangenetik 23: 173–182.
Sasaki, M. and Obara, Y. 1969. Hypersensitivity of lymphocytes in Down's syndrome shown by mixed leukocyte culture experiments.Nature 222: 596–598.
Sasaki, M. S. and Tonomura, A. 1969. Chromosmal sensitivity in Down's syndrome.Jap. J. Human Genet. 14: 81–92.
Sasaki, M. S., Tonomura, A. and Matsubara, S. 1970. Chromosome constitution and its bearing on the chromosomal radiosensitivity in man.Mutat. Res. 10: 617–633.
Schuler, D., Fekete, G. und Dobos, M. 1972. Mit einem alkylierenden Agens (Zitostop)in vitro induzierbare Mutationen bie Malignomen und bie Syndromen, die zur Malignität Disponieren.Humangenetik 16: 329–336.
Segal, D. J. and McCoy, E. E. 1973. Studies on Down's syndrome in tissue culture. I. Growth rate and protein contents of fibroblast cultures.J. Cell Physiol. 83: 85–90.
Steffen, J. and Michalowski, A. 1973. Heterogeneous chromosomal radiosensitivity of phytohemagglutinin-stimulated human blood lymphocytes in culture.Mutat. Res. 17: 367–376.
Younkin, L. H. 1975.In vitro response of lymphocytes to phytohemagglutinin (PHA) as studied with antiserum to PHA. II. Cell cycle analysis.Exp. Cell Res. 90: 374–380.
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Kishi, K. Cell cycle analysis and properties of two sub-populations in PHA responding lymphocytes. Jap J Human Genet 22, 17–26 (1977). https://doi.org/10.1007/BF01908281
- Cell Cycle
- Normal Control
- Normal Cell
- Cell Cycle Analysis
- Chromosome Aberration