Abstract
Acrylamide (AA) is a probable human carcinogen found in several foods. Little information is available regarding exposure of adolescents, a subgroup potentially consuming more AA-rich foods. We investigated the relationship between dietary AA intake and levels of biomarkers of exposure (urinary metabolites and hemoglobin adducts) in 195 non-smoking teenagers of Montreal Island aged 10–17 years. Dietary habits and personal characteristics were documented by questionnaire. AA and its metabolites were quantified in 12-h urine collections by LC-MS/MS. Hemoglobin adducts from 165 blood samples were also analyzed by LC-MS/MS. Most prevalent urinary metabolites were NACP and NACP-S, with respective geometric mean concentrations of 31.2 and 14.2 μmol/mol creatinine. Geometric mean concentrations of AAVal and GAVal (hemoglobin adducts of AA and glycidamide (GA) with N-terminal valine residues) were 45.4 and 45.6 pmol/g globin, respectively. AA intake during the 2 days before urine collection was a significant predictor of NACP+NACP-S urinary concentrations (P<0.0001). AA intakes during the month before blood collection (P<0.0001) and passive smoking (P<0.05) were associated with adduct levels. Levels of hemoglobin adducts were above biomonitoring equivalent values corresponding to a 1 × 10−4 excess cancer risk, which may indicate the need to reduce AA exposure in the population.
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References
Besarantinia A, Pfeifer GP . A review of mechanisms of acrylamide toxicity. Carcinogenesis 2007; 28: 519–528.
Government of Canada Chemical Substances: 2-Propenamide (Acrylamide). http://www.chemicalsubstanceschimiques.gc.ca/challenge-defi/summary-sommaire/batch-lot-5/79-06-1-eng.php 2009.
Tareke E, Rydberg P, Karlsson P, Erikisson S, Törnqvist M . Analysis of acrylamide, a carcinogen formed in heated foodstuffs. J Agric Food Chem 2002; 50: 4998–5006.
Mottram DS, Wedzicha BL, Dodson AT . Acrylamide is formed in the Maillard Reaction. Nature 2002; 419: 448–449.
Stadler RH, Blank I, Varga N, Robert F, Hau J, Guy PA et al. Acrylamide from Maillard reaction products. Nature 2002; 419: 449–450.
McCollister DD, Oyen F, Rowe WK . Toxicology of acrylamide. Toxicol ApplPharmacol 1964; 10: 172–181.
Dearfield KL, Abernathy CO, Ottley MS, Brantner JH, Hayes PF . Acrylamide: its metabolism, developmental and reproductive effects, genotoxicity, and carcinogenicity. Mutat Res 1988; 195: 45–77.
Friedman M . Chemistry, biochemistry and safety of acrylamide. A review. J Agric Food Chem 2003; 51: 4504–4526.
Mucci LA, Dickman PW, Steineck G, Adami HO, Augustsson K . Dietary acrylamide and cancer of the large bowel, kidney, and bladder: absence of an association in a population-based study in Sweden. BJC 2003; 88: 84–89.
Mucci LA, Lindblad P, Steineck G, Adami HO . Dietary acrylamide and risk of renal cancer cell. Int J Cancer 2004; 109: 774–776.
Mucci LA, Sandin S, Bälter K, Adami HO, Magnusson C, Weiderpass E . Acrylamide intake and breast cancer risk in Swedish women. JAMA 2005; 293: 1326–1327.
Mucci LA, Adami HO, Wolk A . Prospective study of dietary acrylamide and risk of colorectal cancer among women. Int J Cancer 2006; 118: 169–173.
Pelucchi C, Galeone C, Dal Maso L, Talamini R, Montella M, Ramazzotti V et al2006 Dietary acrylamide and human cancer. Int J Cancer 2006: 467–471.
Hogervorst JG, Schouten LJ, Konings EJ, Goldbohm A, Van den Brandt PA . A prospective study of dietary acrylamide intake and the risk of endometrial, ovarian and breast cancer. Cancer Epidemiol Biomarkers Prev 2007; 16: 2304–2313.
Wilson KM, Mucci LA, Rosner BA, Willett WC . A prospective study on dietary acrylamide intake and the risk for breast, endometrial and ovarian cancers. Cancer Epidemiol Biomarkers Prev 2010; 19: 2503–2515.
Olesen PT, Frandsen AOH, Frederiksen K, Overvad K, Tjønneland A . Acrylamide exposure and incidence of breast cancer among postmenopausal women in the Danish Diet, Cancer and Health study. Int J Cancer 2008; 122: 2094–2100.
FAO/WHO. Health implications of acrylamide in food. Joint FAO/WHO consultation. Geneva: Switzerland, http://www.who.int/foodsafety/publications/chem/acrylamide_june2002/en/ 2002.
Dybing E, Farmer PB, Andersen M, Fennell TR, Lalljie SP, Müller DJ et al. Human exposure and internal dose assessment of acrylamide in food. Food Chem Toxicol 2005; 43: 365–410.
Heudorf U, Hartmann E, Angerer J . Acrylamide in children – exposure assessment via acrylamide metabolites as biomarkers. Int J Hyg Environ Health 2009; 212: 135–141.
Vesper HW, Caudill SP, Osterloh JD, Meyers T, Scott D, Myers JL . Exposure of the U.S. population to acrylamide in the National Health and Nutrition Examination Survey 2003-2004. EHP 2010; 118: 278–283.
Hartmann EC, Boettcher MI, Schettgen T, Fromme H, Drexler H, Angerer J . Hemoglobin adducts and mercapturic acid excretion of acrylamide and glycidamide in one study population. J Agric Food Chem 2008; 56: 6061–6068.
Normandin L, Bouchard M, Ayotte P, Blanchet C, Becalski A, Bonvalot Y et al. Dietary exposure to acrylamide in adolescents from a Canadian urban center. Food Chem Toxicol 2013; 57: 75–83.
Galanti L . Cotinine urinaire: dosage et applications. RMG 2008; 251: 112–115.
Fennell TR, Snyder RW, Krol WL, Sumner SCJ . Comparison of the hemoglobin adducts formed by administration of N-Methylolacrylamide and Acrylamide to rats. Toxicol Sci 2003; 71: 164–175.
Fennell TR, Sumner SCJ, Snyder RW, Burgess J, Spicer R, Bridson WE et al. Metabolism and hemoglobin adduct formation of acrylamide in humans. Toxicol Sci 2005; 85: 447–459.
Fennell TR, Sumner SCJ, Snyder RW, Burgess J, Friedman MA . Kinetics of elimination of urinary metabolites of acrylamide in humans. Toxicol Sci 2006; 93: 256–267.
Mowrer J, Törnqvist M, Jensen S, Ehrenberg L . Modified Edman degradation applied to haemoglobin for monitoring occupational exposure to alkylating agents. Toxicol Environ Chem 1986; 11: 215–231.
Becalski A, Lau BP, Lewis D, Seaman SW, Hayward S, Sahagian M et al. Acrylamide in French fries: influence of amino acids and sugars. J Agric Food Chem 2004; 52: 3801–3806.
Food and Drug Administration. Survey Data on Acrylamide in Food: Individual Food Products. www.fda.gov/Food/FoodborneIllnessContaminants/ChemicalContaminants/ucm053549.htm 2006a.
Food and Drug Administration. Survey Data on Acrylamide in Food: Total Diet Study Results. www.fda.gov/Food/FoodborneIllnessContaminants/ChemicalContaminants/ucm053566.htm 2006b.
Sumner SCJ, Selvaraj L, Nauhaus SK, Fennell TR . Urinary metabolites from F344 rats and B6C3F1 mice coadministered acrylamide and acrylonitrile for 1 or 5 days. Chem Res Toxicol 1997; 10: 1152–1160.
Tran NL, Barraj LM, Murphy MM, Bi X . Dietary acrylamide exposure andhemoglobin adducts – National Health and Examination Survey (2003-04). Food Chem Toxicol 2010; 48: 3098–3108.
Wilson KM, Vesper HW, Tocco P, Sampson L, Rosén J, Hellenäs KE et al. Validation of a food frequency questionnaire measurement of dietary acrylamide intake using hemoglobin adducts of acrylamide and glycidamide. Cancer Causes Control 2009; 20: 269–278.
Appelhans BM, Milliron BJ, Woolf K, Johnson TJ, Pagoto SL, Schneider KL et al. Socioeconomic status, energy cost and nutrient content of supermarket food purchases. Am J Prev Med 2012; 42: 398–402.
Lipworth L, Sonderman JS, Tarone RE, McLaughlin JK . Review of epidemiologic studies of dietary acrylamide intake and the risk of cancer. Eur J Cancer Prev 2012; 21: 375–386.
Hays SM, Aylward LL . Biomonitoring equivalents (BE) dossier for acrylamide (AA) (CAS No. 79-06-1). Regul Toxicol Pharmacol 2008; 51 (3 Suppl): S57–S67.
Beland FA, Mellick PW, Olson GR, Mendoza MC, Marques MM, Doerge DR . Carcinogenicity of acrylamide in B6C3F(1) mice and F344/N rats from a 2-year drinking water exposure. Food Chem Toxicol 2013; 51: 149–159.
Acknowledgements
This study was funded by Health Canada’s Chemical Management Plan. The support of FRSQ’s Environmental Research Network to the biomarker platform at INSPQ is gratefully acknowledged. Some of the personnel salary was also partly assumed by the Chair in Toxicological Risk Assessment and Management of the University of Montreal. We thank Jean-Yves Sancéau from the Organic Synthesis Service at CHUQ Research Center (http://pfchem.crchul.ulaval.ca/en/index.html) for the synthesis of isotopically labeled acrylamide metabolites.
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Brisson, B., Ayotte, P., Normandin, L. et al. Relation between dietary acrylamide exposure and biomarkers of internal dose in Canadian teenagers. J Expo Sci Environ Epidemiol 24, 215–221 (2014). https://doi.org/10.1038/jes.2013.34
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DOI: https://doi.org/10.1038/jes.2013.34
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