Three new metabolites (2–4), together with one known compound, GTRI-02, (1) were isolated from a fermentation broth of Streptomyces violaceoruber derived from Equus burchelli feces. The structures of the new compounds 2–4 were established using comprehensive NMR spectroscopic data analysis as well as UV, IR and MS data. The anti-inflammatory activity of compounds 1–4 was tested by examining their ability to inhibit nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells. Compound 2 showed a moderate inhibition of NO production with IC50 value of 51.2 μm.
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This work was funded by National Natural Science Foundation of China (Nos 81573327 and 31460005), Basic Scientific Research Fund of Northeastern University, China (No N142002001).
The authors declare no conflict of interest.
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Ma, J., Lei, H., Chen, X. et al. New anti-inflammatory metabolites produced by Streptomyces violaceoruber isolated from Equus burchelli feces. J Antibiot 70, 991–994 (2017). https://doi.org/10.1038/ja.2017.75
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