Ascosteroside D, a new mitochondrial respiration inhibitor discovered by pesticidal screening using insect ADP/ATP carrier protein-expressing Saccharomyces cerevisiae

  • The Journal of Antibiotics volume 71, pages 146148 (2018)
  • doi:10.1038/ja.2017.118
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A new lanostane-type triterpenoid, ascosteroside D, was isolated from a fungus, Aspergillus sp. FKI-6682. It inhibited insect ADP/ATP carrier protein (AAC)-expressing Saccharomyces cerevisiae in glycerol-containing medium, but did not inhibit Δaac S. cerevisiae in glucose-containing medium. It is hypothesized that ascosteroside D inhibits ATP production in mitochondria.

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The study was supported by the Programme for Promotion of Basic and Applied Researches for Innovations in Bio-oriented Industry and the Science and Technology Research Promotion Program for Agriculture, Forestry, Fisheries and Food Industry (26021A). We are grateful to Dr Kenichiro Nagai and Ms Noriko Sato, School of Pharmacy, Kitasato University, for help in obtaining NMR and MS data, and are grateful to Dr Rokuro Masuma for suggestions concerning the experiments.

Author information

Author notes

    • Yoshihiro Watanabe
    •  & Yukihiro Asami

    These authors contributed equally to this work.

    • Takahiro Shiotsuki

    Current address: Faculty of Life and Environmental Science, Shimane University, Matsue, Japan


  1. Graduate School of Infection Control Sciences, Kitasato University, Tokyo, Japan

    • Yoshihiro Watanabe
    • , Yukihiro Asami
    • , Masato Iwatsuki
    • , Kenichi Nonaka
    •  & Kazuro Shiomi
  2. Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan

    • Yukihiro Asami
    • , Masato Iwatsuki
    • , Kenichi Nonaka
    • , Satoshi Ōmura
    •  & Kazuro Shiomi
  3. Department of Chemistry, School of Science, Kitasato University, Kanagawa, Japan

    • Satomi Narusawa
    •  & Shohei Hashimoto
  4. Institute for Genome Research, Tokushima University, Tokushima, Japan

    • Yasuo Shinohara
  5. Insect Growth Regulation Research Unit, National Institute of Agrobiological Sciences, Ibaraki, Japan

    • Takahiro Shiotsuki
  6. Faculty of Bioenvironmental Science, Kyoto Gakuen University, Kyoto, Japan

    • Naoya Ichimaru
  7. Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan

    • Hideto Miyoshi


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Competing interests

The authors declare no conflict of interest.

Corresponding authors

Correspondence to Satoshi Ōmura or Kazuro Shiomi.

Supplementary information

Supplementary Information accompanies the paper on The Journal of Antibiotics website (