Table 1 Summary of 1H and 13C NMR data for 4 and 5 in comparison with 3 in CD3OD

From: Cycloheximide and congeners as inhibitors of eukaryotic protein synthesis from endophytic actinomycetes Streptomyces sps. YIM56132 and YIM56141

  3 a 4 5
Position δH (mult, J in Hz) δ c δH (mult, J in Hz) δc δH (mult, J in Hz) δc
2   172.6   174.8   176.8
3a 2.63–2.79 (2H, m) 40.3 2.40 (m)b 37.7 2.21 (m) 39.9
3b    2.28 (m)c   2.34 (m)b  
4 2.19 (m) 27.2 2.50 (m) 32.5 2.48 (m) 33.7
5a 2.21 (dd, 12.3, 13.0) 35.4 2.28 (2H, m)c 39.1 2.26 (2H, m)b 41.5
5b 2.74 (m)      
6   175.2   176.1   179.2
7a 1.37 (ddd, 11.2, 12.0, 12.0) 31.9 2.21 (m)c 31.3 2.24–2.31 (2H, m)b 31.9
7b 2.07 (m)   2.34 (m)    
8 4.70 (ddd, 3.5, 5.3 11.2) 78.8 6.43 (br t, 6.5) 134.4 6.57 (br t, 6.5) 136.6
9 2.84 (ddd, 5.3, 5.3, 12.5) 49.4   138.7   137.4
10   212.2   204.8   205.6
11 2.45 (m) 28.9 2.41 (m)b 43.9 2.60 (m) 40.5
12a 1.90 (m) 42.5 1.27 (m) 40.8 1.72 (m) 38.2
12b 1.60 (m)   2.02 (m)   1.77 (m)  
13 2.21 (m) 41.3 1.91 (m)d 30.3 2.05 (m) 26.0
14a 2.13 (m) 34.9 1.91 (m)d 35.9 2.17 (m) 34.4
14b 1.76 (m)   2.86 (m)   2.70 (m)  
15 1.26 (d, 7.0) 17.2 1.11 (d, 6.4) 14.9 1.13 (d, 6.5) 16.3
16 0.94 (d, 6.5) 13.6 1.08 (d, 6.5) 21.2 1.09 (d, 6.5) 20.3
  1. a1H and 13C assignments were based on 1H-1H COSY, HMBC and HSQC spectra, which corrected the misassignments reported previously in Morishita et al.15
  2. b−dSignals overlapped.