Abstract
Bacteriocin J46 is a 27-residue polypeptide produced by Lactococcus lactis subsp. cremoris J46 in fermented milk. The natural form of J46 (nJ46) exhibits a broad antimicrobial spectrum. Herein, we produced the synthetic form of J46 (sJ46) by solid-phase chemical synthesis. The biochemical and physico-chemical properties of sJ46, as well as its antimicrobial activity, were found to be identical to those of its natural counterpart nJ46. It showed a potent antimicrobial activity against both lactic acid bacteria and other Gram-positive microorganisms. 1H-NMR conformational analysis of sJ46 indicates that it adopts a flexible random coil structure.
Similar content being viewed by others
Article PDF
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Lasta, S., Fajloun, Z., Darbon, H. et al. Chemical Synthesis and Characterization of J46 Peptide, an Atypical Class IIa Bacteriocin from Lactococcus lactis subsp. cremoris J46 Strain. J Antibiot 61, 89–93 (2008). https://doi.org/10.1038/ja.2008.116
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/ja.2008.116
Keywords
This article is cited by
-
Purification and characterization of pediocin from probiotic Pediococcus pentosaceus GS4, MTCC 12683
Folia Microbiologica (2019)