Studies on Novel Bacterial Translocase I Inhibitors, A-500359s

V. Enhanced Production of Capuramycin and A-500359 A in Streptomyces griseus SANK 60196

Abstract

Streptomyces griseus SANK 60196 produces the novel nucleoside antibiotics A-500359 A, C, D and capuramycin. Enhanced production of capuramycin and A-500359 A was achieved through a number of medium modifications and a series of single colony isolations. The addition of maltose instead of glucose as the carbon source in a primary medium resulted in a 20-fold increase in the productivity of capuramycin. Furthermore, the addition of cobalt chloride (CoCl2) and yeast extract to the medium containing maltose drastically altered the production ratio of A-500359 A to capuramycin. Thus, the yield of A-500359 A increased up to 600 μg/ml in an optimal medium, while the yield in the primary medium was 1 μg/ml.

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Correspondence to Yasunori Muramatsu.

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Muramatsu, Y., Arai, M., Sakaida, Y. et al. Studies on Novel Bacterial Translocase I Inhibitors, A-500359s. J Antibiot 59, 601–606 (2006). https://doi.org/10.1038/ja.2006.81

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Keywords

  • capuramycin
  • translocase I
  • A-500359 A

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