Marine alveolates (MALVs) are diverse and widespread early-branching dinoflagellates, but most knowledge of the group comes from a few cultured species that are generally not abundant in natural samples, or from diversity analyses of PCR-based environmental SSU rRNA gene sequences. To more broadly examine MALV genomes, we generated single cell genome sequences from seven individually isolated cells. Genes expected of heterotrophic eukaryotes were found, with interesting exceptions like presence of proteorhodopsin and vacuolar H+-pyrophosphatase. Phylogenetic analysis of concatenated SSU and LSU rRNA gene sequences provided strong support for the paraphyly of MALV lineages. Dinoflagellate viral nucleoproteins were found only in MALV groups that branched as sister to dinokaryotes. Our findings indicate that multiple independent origins of several characteristics early in dinoflagellate evolution, such as a parasitic life style, underlie the environmental diversity of MALVs, and suggest they have more varied trophic modes than previously thought.
The marine alveolate (MALV) lineages were discovered in the first large molecular surveys of SSU (18 S) rRNA from marine eukaryotes (López-García et al., 2001; Moon-van der Staay et al., 2001). Subsequent environmental SSU PCR-based surveys have consistently shown them to be diverse and widespread, often representing up to 50% of eukaryotic sequences (Chambouvet et al., 2008). SSU rRNA phylogeny has also shown that several ‘syndinian’ dinoflagellates branch with MALVs; these species have been investigated directly, and are all parasites infecting a broad range of host organisms such as cnidarians, fish, crustaceans, and protists (Guillou et al., 2010). Because of this, MALVs are generally assumed to be parasites, and by extension that their abundance in small size fractions may reflect large numbers of infectious cells (perhaps blooms following host blooms), which are thought to have ecological importance in controlling host populations. This is reinforced by recent surveys that show MALVs make up ~90% of putative parasites in the piconanoplankton globally (de Vargas et al., 2015). However, most MALVs are only known from these environmental SSU rRNA gene sequences, and those that have been characterized further are relatively rare and do not represent all MALV subgroups. As only SSU rRNA is generally known, the phylogenetic position of MALVs is also partially uncertain: they are typically basal to dinokaryotes (that is, dinoflagellates with permanently condensed chromosomes), but may be monophyletic or paraphyletic with dinokaryotes branching among them, and in either case the phylogenies lack support (Guillou et al., 2008; Skovgaard et al., 2009; Horiguchi, 2015). Overall, most MALV diversity is unexamined and how MALV groups are related to one another and to dinokaryotes remains poorly resolved.
Results and discussion
Using manual single cell isolation (or isolation of infected hosts) and fluorescence-activated cell sorting (FACS), we surveyed heterotrophic eukaryotes and identified cells corresponding to MALVs by SSU rRNA phylogeny, resulting in seven distantly related species from the Northeastern Subarctic Pacific Ocean (Line P) and from Monterey Bay out to the edge of the North Pacific Subtropical Gyre (Line 67; for collection and screening details, see Supplementary Table 1 and Supplementary Materials and methods). Two manual isolations were morphologically identifiable hosts (Figure 1); a copepod (L67-3) in the process of bursting to release spores identified as the dinoflagellate Chytriodinium, and a tintinnid ciliate (L67-6) that yielded two distinct MALV phylotypes (93.8% similarity, likely from two different MALV II species). Manually isolated cells L67-1 and L67-4 are inferred to be host-free and fit within size fractions that previously yielded MALV sequences: L67-1 is spherical-shaped with a diameter of 13.8 μm and with greenish inclusions, L67-4 is colourless, spindle-shaped, and 14.5 × 6.3 μm (Figure 1). One isolation (L67-5) was large and inferred to be a host, which could not be identified, and the remaining two cells (L67-2 and LP-1) were isolated by FACS.
Genomic data from each isolation was generated, assembled, and screened for contamination (for example, from bacteria or host genomes) using a combination of BLAST-based and phylogenetic approaches (that is, phylogenetic trees were inferred from gene alignments of a broad range of taxa including the MALVs Hematodinium and Amoebophrya; for details see Supplementary Methods). Because dinoflagellate genomes are large and gene-sparse (Wisecaver and Hackett, 2011), we expected to recover partial genomes and focused only on regions encoding a recognizable gene, to avoid including non-coding regions from contaminants. This resulted in 0.5–3.1 Mb per cell confidently assigned to MALVs, encoding between 97 to 917 predicted genes, 30 to 60% of which corresponded to KEGG hits (Figure 1; Supplementary Tables 2 and 3; Supplementary Figure 1). Estimated coverage of eukaryotic conserved single copy genes using BUSCO showed 86 to 99% were missing. The genomic coverage was therefore low, as expected, but an improvement over only SSU rRNA.
The proteins that were identified matched the expectations for heterotrophic eukaryotes since no plastid-related protein was found, as observed in Hematodinium (Gornik et al., 2015), but mitochondrial proteins were detected. Interestingly, L67-5 encoded a photoactive proton pump, proteorhodopsin, most similar to homologues from Oxyrrhis and Alexandrium, and five samples encoded a vacuolar H+-pyrophosphatase (L67-1–L67-3, L67-5, LP-1). These two proteins have been hypothesized to function together in dinoflagellates to generate energy in the form of pyrophosphate (Slamovits et al., 2011). The presence of light-driven proton pumps in MALVs suggests an early origin of this system in the dinoflagellate lineage, and that some MALVs may be able to use light for energy (despite the absence of plastids), as this is the suspected function of these proteins in other non-photosynthetic dinoflagellates.
Assemblies from all seven cells include the rRNA operon, generally complete. Mapping Tara Oceans amplicon data to their SSU V9 region demonstrated that two cell types were moderately abundant (>27 000 and >77 000 reads for L67-2 and L67-4, respectively; Figure 1) and widespread in pico- and nano-plankton size fractions (Figures 2a and b). This distribution is beyond Tara’s sampling representation bias (that is, mostly pico- and nano-plankton were sampled; Figure 2c), so if MALVs are parasites, then they predominantly exist as free-living spores rather than host-associated (consistent with the image of L67-4; Figure 1). Four other phylotypes were rare (<5 reads; Figure 1) in the Tara Oceans data. Notably, however, North Pacific data from Tara are unavailable, so these four phylotypes may be endemic to the North Pacific (for example, parasites of North Pacific hosts).
Originally, MALVs were divided into two major groups: MALV I including Ichthyodinium and Duboscquella (Harada et al., 2007; Skovgaard et al., 2009), and MALV II, including Syndinium, Hematodinium and Amoebophrya (Skovgaard et al., 2005). Both groups were subsequently subdivided further, but the relationships between subgroups remained uncertain (Guillou et al., 2008; Skovgaard et al., 2009; Horiguchi, 2015; see also Supplementary Figure 2). The concatenated SSU/LSU tree (Figure 1) recovers four strongly-supported subgroups: MALV Ia, Ib, II and IV (following the names of Guillou et al., 2008, but distinguishing two phylogenetically distant subgroups of MALV I). Most interestingly MALVs are paraphyletic, with MALV II/IV branching as sister to dinokaryotes (core dinoflagellates). Currently, no synapomorphy for a dinokaryote-MALV-II/IV group is known, but one possibility worth investigation is the presence of DVNPs (dinoflagellate viral nucleoproteins). Within MALVs, DVNPs are found in Hematodinium (MALV IV), Amoebophrya, L67-3, and LP-1 (MALV II; Gornik et al., 2012; Marinov and Lynch, 2015; Supplementary Table 3), but so far, no evidence for their presence in MALV I has been obtained.
The phylogeny raises interesting questions about the distribution of parasitism in early alveolate evolution. In particular, the paraphyletic relationship of seemingly aplastidal parasites at the base of dinoflagellates could suggest a parasitic ancestral state. However, this scenario leaves unexplained many specific shared similarities in the plastids of dinokaryotes, apicomplexans and chrompodellids (Janouškovec et al., 2015), and is inconsistent with the apparent ancestral function of the apical complex as a feeding apparatus (Okamoto and Keeling, 2014a). Alternatively, a mixotrophic ancestry that included both the plastid and the apical complex for feeding is possible; in this case, one must explain an apparently strong predilection to loosing photosynthesis and transitioning to parasitism by modifying the feeding apparatus. A recent analysis of metabolic redundancies within the apicomplexan/dinoflagellate clade also argued for a recent plastid gain, before the divergence of these groups but after they diverged from ciliates (Waller et al., 2015), which is also consistent with this view.
Despite their abundance, distribution, and inferred ecological significance, MALVs remain mysterious. They are assumed to be parasites, and the microscopic and genomic data described here are consistent with this, and in some cases identify new hosts. Their exact evolutionary relationship to dinokaryotes has also been controversial (Guillou et al., 2008; Massana et al., 2008; Horiguchi, 2015), and our data provide strong support for their paraphyly. These insights impact how we reconstruct the evolution of plastids and parasitism in MALVs and dinoflagellates. Finally, surprising aspects of gene content and potential endemism, reveal possible complexities in trophic modes and ecology that serve as a springboard for future investigations.
Raw reads have been submitted to GenBank under accession numbers SRR5145189 and SRR5177625–SRR5177630, and the assembled contigs can be accessed via the Dryad Digital Repository http://doi.org/10.5061/dryad.hg56n.
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We thank the captain and crew of the R/V Western Flyer, M Blum, F Chavez, V Jimenez, JT Pennington, JM Smith, S Sudek, J Swalwell, C Wahl and S Wilken, for logistical assistance before and during the cruises. This work was supported by a grant from the Gordon and Betty Moore Foundation (GBMF3307) to PJK, TAR, AZW and AES. Sequencing for L67-2 was performed through a JGI Technology Development Program grant to TAR, AZW, PJK and AES. Ship time and sorting methods were supported by a grant from the David and Lucile Packard Foundation through MBARI and GBMF3788 to AZW. JdC was supported by a Marie Curie International Outgoing Fellowship grant (FP7-PEOPLE-2012-IOF – 331450 CAARL), and AK, NO, MK and JdC were supported by a grant from the Tula Foundation to the Centre for Microbial Biodiversity and Evolution at UBC.
The authors declare no conflict of interest.
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Strassert, J., Karnkowska, A., Hehenberger, E. et al. Single cell genomics of uncultured marine alveolates shows paraphyly of basal dinoflagellates. ISME J 12, 304–308 (2018). https://doi.org/10.1038/ismej.2017.167
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