Fluorescence in situ hybridization (FISH) micrograph of microorganisms recovered from a methane seep sediment incubation. Cells targeted by the general bacterial probe, EUB338, are shown in green (FITC); cells targeted by the specific probe for Izimaplasma (Izzy659) are shown in yellow (combined FITC and Cy3 fluorescence). The scale bar in both images is 10 μm. (a) FISH image of microorganisms from the initial sediment sample used for the Izimaplasma enrichment. (b) Microbial diversity after 6 weeks of cultivation in glucose-amended enrichment media.