Figure 5 | The ISME Journal

Figure 5

From: Functional characterization of polysaccharide utilization loci in the marine BacteroidetesGramella forsetii’ KT0803

Figure 5

Proposed pathway for the decomposition of alpha-1,4-glucans in ‘G. forsetii’ (a), organization of the cluster and its protein profile (b). (a) An alpha-1,4-linked glucose-polymer (glycogen, starch and amylose) is converted into oligomeric dextrin (by GH13 alpha-amylase, GFO_2132, GFO_2141 or GFO_2133), dimeric maltose (by dextrinase function of GFO_2133), glucose 1-phosphate (Glc-1P; by GH65 maltose phosphorylase, GFO_2134) and finally glucose-6-phosphate (Glc-6P; by PgmB, GFO_2135). The latter metabolite can be directly used by glycolysis and metabolized into pyruvate (Pyr). (b) Intracellular components contained in the operon located upstream were upregulated in both glucose- and laminarin-supplied cultures (†, highest protein quantity changes in glucose-grown cultures), whereas membrane proteins of the downstream located operon exhibited upregulation particularly in laminarin-grown cultures (‡P<0.01). Except for the two alpha-amylases (GFO_2132 and GFO_2141) and the LacI family regulator, all genes were found to be expressed in this proteome study. According to the predicted subcellular localization, log2 ratio values are shown for the respective subcellular fraction of each identified protein (error bars: s.d.; protein ratios: see Supplementary Table S9). Locus tags are given as numbers below the genes. hyp, hypothetical protein; gapA, glyceraldehyde-3-phosphate dehydrogenase A; pfkA, 6-phosphofructokinase; gh, glycoside hydrolase family; pgmB, beta-phosphoglucomutase; mfs, MFS permease—possibly alpha-glucoside transporter; lacI, LacI family transcriptional regulator; susC, SusC-like TBDR; susD, SusD-family protein; susE, putative SusE-like protein.

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