Figure 1 | The ISME Journal

Figure 1

From: Functional characterization of polysaccharide utilization loci in the marine BacteroidetesGramella forsetii’ KT0803

Figure 1

Expression profile (a) and gene organization of the laminarin utilization operon. (a) Protein ratios of the intracellular soluble and membrane protein fractions of cultures grown on laminarin compared with alginate are shown. Except for a signal transduction system (GFO_3462), all genes of the laminarin operon were overexpressed in the presence of laminarin. Consistency in protein levels is highlighted by the gray background that combines all data points of the corresponding substrate and indicates a uniform cluster regulation. Error bars refer to the s.d. of all biological replicates (n=3), median-centered protein ratios are given in Supplementary Table S4. (b) The gene organization of the laminarin-specific PUL in ‘G. forsetii’ is conserved among other marine Flavobacteriaceae as exemplified. Identity/similarity values are listed in Supplementary Table S9. htcs, hybrid two-component system; susC, SusC-like TBDR; susD, SusD-family protein; pkd, PKD-domain-containing protein; gh, glycoside hydrolase.

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